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Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats
Carbon dioxide (CO(2)) is commonly used to kill laboratory rats. Rats find CO(2) aversive and aversion varies between individuals, indicating that rats vary in CO(2) sensitivity. Healthy humans experience feelings of anxiety at concentrations similar to those avoided by rats, and these feelings are...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908729/ https://www.ncbi.nlm.nih.gov/pubmed/31831816 http://dx.doi.org/10.1038/s41598-019-55493-0 |
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author | Améndola, Lucía Ratuski, Anna Weary, Daniel M. |
author_facet | Améndola, Lucía Ratuski, Anna Weary, Daniel M. |
author_sort | Améndola, Lucía |
collection | PubMed |
description | Carbon dioxide (CO(2)) is commonly used to kill laboratory rats. Rats find CO(2) aversive and aversion varies between individuals, indicating that rats vary in CO(2) sensitivity. Healthy humans experience feelings of anxiety at concentrations similar to those avoided by rats, and these feelings are diminished by the administration of benzodiazepines. Our aim was to assess the effects of the benzodiazepine midazolam on individual thresholds of rat aversion to CO(2). Six female Sprague Dawley rats were repeatedly exposed to CO(2) gradual-fill in approach-avoidance testing. The first three exposures were to a control-treatment followed by three exposures to midazolam (0.375 mg/kg). Within each treatment aversion to CO(2) was not affected by exposure number; however, tolerance increased from an average of 10.7% CO(2) avoided during control sessions, to 15.5% CO(2) avoided when treated with midazolam. These results indicate that rats experience anxiety when exposed to CO(2), and that variation in rat CO(2) sensitivity is driven by individual differences in the onset of these feelings of anxiety. No rat tolerated CO(2) concentrations required to induce loss of consciousness. |
format | Online Article Text |
id | pubmed-6908729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69087292019-12-16 Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats Améndola, Lucía Ratuski, Anna Weary, Daniel M. Sci Rep Article Carbon dioxide (CO(2)) is commonly used to kill laboratory rats. Rats find CO(2) aversive and aversion varies between individuals, indicating that rats vary in CO(2) sensitivity. Healthy humans experience feelings of anxiety at concentrations similar to those avoided by rats, and these feelings are diminished by the administration of benzodiazepines. Our aim was to assess the effects of the benzodiazepine midazolam on individual thresholds of rat aversion to CO(2). Six female Sprague Dawley rats were repeatedly exposed to CO(2) gradual-fill in approach-avoidance testing. The first three exposures were to a control-treatment followed by three exposures to midazolam (0.375 mg/kg). Within each treatment aversion to CO(2) was not affected by exposure number; however, tolerance increased from an average of 10.7% CO(2) avoided during control sessions, to 15.5% CO(2) avoided when treated with midazolam. These results indicate that rats experience anxiety when exposed to CO(2), and that variation in rat CO(2) sensitivity is driven by individual differences in the onset of these feelings of anxiety. No rat tolerated CO(2) concentrations required to induce loss of consciousness. Nature Publishing Group UK 2019-12-12 /pmc/articles/PMC6908729/ /pubmed/31831816 http://dx.doi.org/10.1038/s41598-019-55493-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Améndola, Lucía Ratuski, Anna Weary, Daniel M. Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title | Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title_full | Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title_fullStr | Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title_full_unstemmed | Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title_short | Variation in the onset of CO(2)-induced anxiety in female Sprague Dawley rats |
title_sort | variation in the onset of co(2)-induced anxiety in female sprague dawley rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908729/ https://www.ncbi.nlm.nih.gov/pubmed/31831816 http://dx.doi.org/10.1038/s41598-019-55493-0 |
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