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Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking
INTRODUCTION: Cocaine dependence affects millions of individuals worldwide; however, there are no pharmacotherapeutic and/or diagnostic solutions. Recent evidence suggests a role for lipid signaling in the development and maintenance of addiction, highlighting the need to understand how lipid remode...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908860/ https://www.ncbi.nlm.nih.gov/pubmed/31701674 http://dx.doi.org/10.1002/brb3.1451 |
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author | Pati, Sumitra Angel, Peggi Drake, Richard R. Wagner, John J. Cummings, Brian S. |
author_facet | Pati, Sumitra Angel, Peggi Drake, Richard R. Wagner, John J. Cummings, Brian S. |
author_sort | Pati, Sumitra |
collection | PubMed |
description | INTRODUCTION: Cocaine dependence affects millions of individuals worldwide; however, there are no pharmacotherapeutic and/or diagnostic solutions. Recent evidence suggests a role for lipid signaling in the development and maintenance of addiction, highlighting the need to understand how lipid remodeling mediates neuroadaptation after cocaine exposure. METHODS: This study utilized shotgun lipidomics to assess cocaine‐induced lipid remodeling in rats using a novel behavioral regimen that incorporated multiple sessions of extinction training and reinstatement testing. RESULTS: Mass spectrometric imaging demonstrated widespread decreases in phospholipid (PL) abundance throughout the brain, and high‐spatial resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry indicated hippocampus‐specific PL alterations following cocaine exposure. We analyzed the expression of genes involved in hippocampal lipid metabolism and observed region‐specific regulation. In addition, we found that cocaine exposure differentially regulates mitochondrial biogenesis in the brain. CONCLUSIONS: This work presents a comprehensive lipidomic assessment of cocaine‐induced lipid remodeling in the rat brain. Further, these findings indicate a potential interplay between CNS energetics and differential lipid regulation and suggest a role for cocaine in the maintenance of energy homeostasis. |
format | Online Article Text |
id | pubmed-6908860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69088602019-12-20 Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking Pati, Sumitra Angel, Peggi Drake, Richard R. Wagner, John J. Cummings, Brian S. Brain Behav Original Research INTRODUCTION: Cocaine dependence affects millions of individuals worldwide; however, there are no pharmacotherapeutic and/or diagnostic solutions. Recent evidence suggests a role for lipid signaling in the development and maintenance of addiction, highlighting the need to understand how lipid remodeling mediates neuroadaptation after cocaine exposure. METHODS: This study utilized shotgun lipidomics to assess cocaine‐induced lipid remodeling in rats using a novel behavioral regimen that incorporated multiple sessions of extinction training and reinstatement testing. RESULTS: Mass spectrometric imaging demonstrated widespread decreases in phospholipid (PL) abundance throughout the brain, and high‐spatial resolution matrix‐assisted laser desorption/ionization Fourier‐transform ion cyclotron resonance mass spectrometry indicated hippocampus‐specific PL alterations following cocaine exposure. We analyzed the expression of genes involved in hippocampal lipid metabolism and observed region‐specific regulation. In addition, we found that cocaine exposure differentially regulates mitochondrial biogenesis in the brain. CONCLUSIONS: This work presents a comprehensive lipidomic assessment of cocaine‐induced lipid remodeling in the rat brain. Further, these findings indicate a potential interplay between CNS energetics and differential lipid regulation and suggest a role for cocaine in the maintenance of energy homeostasis. John Wiley and Sons Inc. 2019-11-07 /pmc/articles/PMC6908860/ /pubmed/31701674 http://dx.doi.org/10.1002/brb3.1451 Text en © 2019 The Authors. Brain and Behavior published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Pati, Sumitra Angel, Peggi Drake, Richard R. Wagner, John J. Cummings, Brian S. Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title | Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title_full | Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title_fullStr | Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title_full_unstemmed | Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title_short | Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
title_sort | lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug‐seeking |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6908860/ https://www.ncbi.nlm.nih.gov/pubmed/31701674 http://dx.doi.org/10.1002/brb3.1451 |
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