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Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909058/ https://www.ncbi.nlm.nih.gov/pubmed/31871922 http://dx.doi.org/10.1016/j.btre.2019.e00392 |
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author | Sasaki, Mitsuki Takagi, Akari Ota, Shuhei Kawano, Shigeyuki Sasaki, Daisaku Asayama, Munehiko |
author_facet | Sasaki, Mitsuki Takagi, Akari Ota, Shuhei Kawano, Shigeyuki Sasaki, Daisaku Asayama, Munehiko |
author_sort | Sasaki, Mitsuki |
collection | PubMed |
description | A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with 2 % CO(2)-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivation conditions of BG11, BG11–N, BG11–P (phosphate depleted), and BG11–N–P (nitrogen and phosphate depleted). Fatty-methyl-esters (FAMEs), methyl-esterification of total lipids, consisted of abundant saturated C16 and unsaturated C18 fatty acids under the culture conditions. Cell spot assays on BG11 plates revealed the resistance of cells to pH 2–11, high temperatures of 50–70 °C, ultraviolet irradiation, and drought, under different culture conditions, thereby suggesting the biological significance of lipid and EPS accumulation. The prospects of BX1.5 as a dual producer has also been discussed for biorefineries. |
format | Online Article Text |
id | pubmed-6909058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69090582019-12-23 Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions Sasaki, Mitsuki Takagi, Akari Ota, Shuhei Kawano, Shigeyuki Sasaki, Daisaku Asayama, Munehiko Biotechnol Rep (Amst) Research Article A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with 2 % CO(2)-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivation conditions of BG11, BG11–N, BG11–P (phosphate depleted), and BG11–N–P (nitrogen and phosphate depleted). Fatty-methyl-esters (FAMEs), methyl-esterification of total lipids, consisted of abundant saturated C16 and unsaturated C18 fatty acids under the culture conditions. Cell spot assays on BG11 plates revealed the resistance of cells to pH 2–11, high temperatures of 50–70 °C, ultraviolet irradiation, and drought, under different culture conditions, thereby suggesting the biological significance of lipid and EPS accumulation. The prospects of BX1.5 as a dual producer has also been discussed for biorefineries. Elsevier 2019-11-21 /pmc/articles/PMC6909058/ /pubmed/31871922 http://dx.doi.org/10.1016/j.btre.2019.e00392 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Sasaki, Mitsuki Takagi, Akari Ota, Shuhei Kawano, Shigeyuki Sasaki, Daisaku Asayama, Munehiko Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title | Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title_full | Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title_fullStr | Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title_full_unstemmed | Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title_short | Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions |
title_sort | coproduction of lipids and extracellular polysaccharides from the novel green alga parachlorella sp. bx1.5 depending on cultivation conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909058/ https://www.ncbi.nlm.nih.gov/pubmed/31871922 http://dx.doi.org/10.1016/j.btre.2019.e00392 |
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