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Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions

A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with...

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Autores principales: Sasaki, Mitsuki, Takagi, Akari, Ota, Shuhei, Kawano, Shigeyuki, Sasaki, Daisaku, Asayama, Munehiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909058/
https://www.ncbi.nlm.nih.gov/pubmed/31871922
http://dx.doi.org/10.1016/j.btre.2019.e00392
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author Sasaki, Mitsuki
Takagi, Akari
Ota, Shuhei
Kawano, Shigeyuki
Sasaki, Daisaku
Asayama, Munehiko
author_facet Sasaki, Mitsuki
Takagi, Akari
Ota, Shuhei
Kawano, Shigeyuki
Sasaki, Daisaku
Asayama, Munehiko
author_sort Sasaki, Mitsuki
collection PubMed
description A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with 2 % CO(2)-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivation conditions of BG11, BG11–N, BG11–P (phosphate depleted), and BG11–N–P (nitrogen and phosphate depleted). Fatty-methyl-esters (FAMEs), methyl-esterification of total lipids, consisted of abundant saturated C16 and unsaturated C18 fatty acids under the culture conditions. Cell spot assays on BG11 plates revealed the resistance of cells to pH 2–11, high temperatures of 50–70 °C, ultraviolet irradiation, and drought, under different culture conditions, thereby suggesting the biological significance of lipid and EPS accumulation. The prospects of BX1.5 as a dual producer has also been discussed for biorefineries.
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spelling pubmed-69090582019-12-23 Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions Sasaki, Mitsuki Takagi, Akari Ota, Shuhei Kawano, Shigeyuki Sasaki, Daisaku Asayama, Munehiko Biotechnol Rep (Amst) Research Article A novel strain of microalga Parachlorella sp. BX1.5 was isolated and its unique properties of producing lipids and extracellular polysaccharides (EPS) characterized. The cells could extracellularly produce a large amount of acidic EPS, when cultured in nitrogen-deficient BG11(0) medium (BG11–N) with 2 % CO(2)-air supply. The main component of intracellularly accumulated lipids was triacylglycerol (TAG), depending on the different cultivation conditions of BG11, BG11–N, BG11–P (phosphate depleted), and BG11–N–P (nitrogen and phosphate depleted). Fatty-methyl-esters (FAMEs), methyl-esterification of total lipids, consisted of abundant saturated C16 and unsaturated C18 fatty acids under the culture conditions. Cell spot assays on BG11 plates revealed the resistance of cells to pH 2–11, high temperatures of 50–70 °C, ultraviolet irradiation, and drought, under different culture conditions, thereby suggesting the biological significance of lipid and EPS accumulation. The prospects of BX1.5 as a dual producer has also been discussed for biorefineries. Elsevier 2019-11-21 /pmc/articles/PMC6909058/ /pubmed/31871922 http://dx.doi.org/10.1016/j.btre.2019.e00392 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Sasaki, Mitsuki
Takagi, Akari
Ota, Shuhei
Kawano, Shigeyuki
Sasaki, Daisaku
Asayama, Munehiko
Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title_full Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title_fullStr Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title_full_unstemmed Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title_short Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1.5 depending on cultivation conditions
title_sort coproduction of lipids and extracellular polysaccharides from the novel green alga parachlorella sp. bx1.5 depending on cultivation conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909058/
https://www.ncbi.nlm.nih.gov/pubmed/31871922
http://dx.doi.org/10.1016/j.btre.2019.e00392
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