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Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial

High-protein diet is a promising strategy for diabetes treatment supporting body weight control, improving glycaemic status, cardiovascular risk factors and reducing liver fat. Here, we investigated effects of diets high in animal (AP) or plant (PP) protein on oxidative stress and antioxidant status...

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Autores principales: Pivovarova-Ramich, Olga, Markova, Mariya, Weber, Daniela, Sucher, Stephanie, Hornemann, Silke, Rudovich, Natalia, Raila, Jens, Sunaga-Franze, Daniele, Sauer, Sascha, Rohn, Sascha, Pfeiffer, Andreas F.H., Grune, Tilman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909130/
https://www.ncbi.nlm.nih.gov/pubmed/31926623
http://dx.doi.org/10.1016/j.redox.2019.101397
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author Pivovarova-Ramich, Olga
Markova, Mariya
Weber, Daniela
Sucher, Stephanie
Hornemann, Silke
Rudovich, Natalia
Raila, Jens
Sunaga-Franze, Daniele
Sauer, Sascha
Rohn, Sascha
Pfeiffer, Andreas F.H.
Grune, Tilman
author_facet Pivovarova-Ramich, Olga
Markova, Mariya
Weber, Daniela
Sucher, Stephanie
Hornemann, Silke
Rudovich, Natalia
Raila, Jens
Sunaga-Franze, Daniele
Sauer, Sascha
Rohn, Sascha
Pfeiffer, Andreas F.H.
Grune, Tilman
author_sort Pivovarova-Ramich, Olga
collection PubMed
description High-protein diet is a promising strategy for diabetes treatment supporting body weight control, improving glycaemic status, cardiovascular risk factors and reducing liver fat. Here, we investigated effects of diets high in animal (AP) or plant (PP) protein on oxidative stress and antioxidant status in individuals with type 2 diabetes (T2DM). 37 obese individuals (age 64.3 ± 1.0 years) with T2DM were randomized to an isocaloric diet (30 energy(E)% protein, 30 E% fat and 40 E% carbohydrates) rich in AP or PP for 6 weeks. Markers of oxidative and nitrosative stress and antioxidant status in plasma and nitrate/nitrite levels in urine were assessed. Gene expression in subcutaneous adipose tissue (SAT) was analysed by RNA-Seq and real-time PCR. Both AP and PP diets similarly reduced plasma levels of malondialdehyde (P(AP) = 0.003, P(PP) = 1.6 × 10(−4)) and protein carbonyls (P(AP) = 1.2 × 10(−4), P(PP) = 3.0 × 10(−5)) over 6 weeks. Nitrotyrosine (NT) increased upon both AP and PP diets (P(AP) = 0.005, P(PP) = 0.004). SAT expression of genes involved in nitric oxide (NO) and oxidative stress metabolism and urine NO metabolite (nitrate/nitrite) levels were not changed upon both diets. Plasma levels of carotenoids increased upon PP diet, whereas retinol, alpha- and gamma-tocopherol slightly decreased upon both diets. AP and PP diets similarly improve oxidative stress but increase nitrosative stress markers in individuals with T2DM. Mechanisms of the NT regulation upon high-protein diets need further investigation.
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spelling pubmed-69091302019-12-23 Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial Pivovarova-Ramich, Olga Markova, Mariya Weber, Daniela Sucher, Stephanie Hornemann, Silke Rudovich, Natalia Raila, Jens Sunaga-Franze, Daniele Sauer, Sascha Rohn, Sascha Pfeiffer, Andreas F.H. Grune, Tilman Redox Biol Research Paper High-protein diet is a promising strategy for diabetes treatment supporting body weight control, improving glycaemic status, cardiovascular risk factors and reducing liver fat. Here, we investigated effects of diets high in animal (AP) or plant (PP) protein on oxidative stress and antioxidant status in individuals with type 2 diabetes (T2DM). 37 obese individuals (age 64.3 ± 1.0 years) with T2DM were randomized to an isocaloric diet (30 energy(E)% protein, 30 E% fat and 40 E% carbohydrates) rich in AP or PP for 6 weeks. Markers of oxidative and nitrosative stress and antioxidant status in plasma and nitrate/nitrite levels in urine were assessed. Gene expression in subcutaneous adipose tissue (SAT) was analysed by RNA-Seq and real-time PCR. Both AP and PP diets similarly reduced plasma levels of malondialdehyde (P(AP) = 0.003, P(PP) = 1.6 × 10(−4)) and protein carbonyls (P(AP) = 1.2 × 10(−4), P(PP) = 3.0 × 10(−5)) over 6 weeks. Nitrotyrosine (NT) increased upon both AP and PP diets (P(AP) = 0.005, P(PP) = 0.004). SAT expression of genes involved in nitric oxide (NO) and oxidative stress metabolism and urine NO metabolite (nitrate/nitrite) levels were not changed upon both diets. Plasma levels of carotenoids increased upon PP diet, whereas retinol, alpha- and gamma-tocopherol slightly decreased upon both diets. AP and PP diets similarly improve oxidative stress but increase nitrosative stress markers in individuals with T2DM. Mechanisms of the NT regulation upon high-protein diets need further investigation. Elsevier 2019-11-28 /pmc/articles/PMC6909130/ /pubmed/31926623 http://dx.doi.org/10.1016/j.redox.2019.101397 Text en © 2019 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Paper
Pivovarova-Ramich, Olga
Markova, Mariya
Weber, Daniela
Sucher, Stephanie
Hornemann, Silke
Rudovich, Natalia
Raila, Jens
Sunaga-Franze, Daniele
Sauer, Sascha
Rohn, Sascha
Pfeiffer, Andreas F.H.
Grune, Tilman
Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title_full Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title_fullStr Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title_full_unstemmed Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title_short Effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: A randomized clinical trial
title_sort effects of diets high in animal or plant protein on oxidative stress in individuals with type 2 diabetes: a randomized clinical trial
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909130/
https://www.ncbi.nlm.nih.gov/pubmed/31926623
http://dx.doi.org/10.1016/j.redox.2019.101397
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