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Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
[Image: see text] Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester...
| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909233/ https://www.ncbi.nlm.nih.gov/pubmed/31773957 http://dx.doi.org/10.1021/jacs.9b09813 |
| _version_ | 1783478916471586816 |
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| author | Murfin, Lloyd C. Weber, Maria Park, Sang Jun Kim, Won Tae Lopez-Alled, Carlos M. McMullin, Claire L. Pradaux-Caggiano, Fabienne Lyall, Catherine L. Kociok-Köhn, Gabriele Wenk, Jannis Bull, Steven D. Yoon, Juyoung Kim, Hwan Myung James, Tony D. Lewis, Simon E. |
| author_facet | Murfin, Lloyd C. Weber, Maria Park, Sang Jun Kim, Won Tae Lopez-Alled, Carlos M. McMullin, Claire L. Pradaux-Caggiano, Fabienne Lyall, Catherine L. Kociok-Köhn, Gabriele Wenk, Jannis Bull, Steven D. Yoon, Juyoung Kim, Hwan Myung James, Tony D. Lewis, Simon E. |
| author_sort | Murfin, Lloyd C. |
| collection | PubMed |
| description | [Image: see text] Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability. |
| format | Online Article Text |
| id | pubmed-6909233 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | American Chemical Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-69092332019-12-19 Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy Murfin, Lloyd C. Weber, Maria Park, Sang Jun Kim, Won Tae Lopez-Alled, Carlos M. McMullin, Claire L. Pradaux-Caggiano, Fabienne Lyall, Catherine L. Kociok-Köhn, Gabriele Wenk, Jannis Bull, Steven D. Yoon, Juyoung Kim, Hwan Myung James, Tony D. Lewis, Simon E. J Am Chem Soc [Image: see text] Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability. American Chemical Society 2019-11-27 2019-12-11 /pmc/articles/PMC6909233/ /pubmed/31773957 http://dx.doi.org/10.1021/jacs.9b09813 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
| spellingShingle | Murfin, Lloyd C. Weber, Maria Park, Sang Jun Kim, Won Tae Lopez-Alled, Carlos M. McMullin, Claire L. Pradaux-Caggiano, Fabienne Lyall, Catherine L. Kociok-Köhn, Gabriele Wenk, Jannis Bull, Steven D. Yoon, Juyoung Kim, Hwan Myung James, Tony D. Lewis, Simon E. Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title | Azulene-Derived Fluorescent
Probe for Bioimaging:
Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title_full | Azulene-Derived Fluorescent
Probe for Bioimaging:
Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title_fullStr | Azulene-Derived Fluorescent
Probe for Bioimaging:
Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title_full_unstemmed | Azulene-Derived Fluorescent
Probe for Bioimaging:
Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title_short | Azulene-Derived Fluorescent
Probe for Bioimaging:
Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy |
| title_sort | azulene-derived fluorescent
probe for bioimaging:
detection of reactive oxygen and nitrogen species by two-photon microscopy |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909233/ https://www.ncbi.nlm.nih.gov/pubmed/31773957 http://dx.doi.org/10.1021/jacs.9b09813 |
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