Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities

Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing...

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Autores principales: Halatchev, Ilia G., O’Donnell, David, Hibberd, Matthew C., Gordon, Jeffrey I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909537/
https://www.ncbi.nlm.nih.gov/pubmed/31831058
http://dx.doi.org/10.1186/s40168-019-0769-4
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author Halatchev, Ilia G.
O’Donnell, David
Hibberd, Matthew C.
Gordon, Jeffrey I.
author_facet Halatchev, Ilia G.
O’Donnell, David
Hibberd, Matthew C.
Gordon, Jeffrey I.
author_sort Halatchev, Ilia G.
collection PubMed
description Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized.
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spelling pubmed-69095372019-12-19 Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities Halatchev, Ilia G. O’Donnell, David Hibberd, Matthew C. Gordon, Jeffrey I. Microbiome Methodology Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized. BioMed Central 2019-12-12 /pmc/articles/PMC6909537/ /pubmed/31831058 http://dx.doi.org/10.1186/s40168-019-0769-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology
Halatchev, Ilia G.
O’Donnell, David
Hibberd, Matthew C.
Gordon, Jeffrey I.
Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title_full Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title_fullStr Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title_full_unstemmed Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title_short Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
title_sort applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909537/
https://www.ncbi.nlm.nih.gov/pubmed/31831058
http://dx.doi.org/10.1186/s40168-019-0769-4
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