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Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function

BACKGROUND: Porcine circovirus (PCV) disease caused by PCV type 2 (PCV2) is mainly attributed to immunosuppression and immune damage. PCV2 can infect vascular endothelial cells and induce high expression of endothelial IL-8. Dendritic cells (DCs), as professional antigen-presenting cells, can not on...

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Autores principales: Liu, Shiyu, Li, Qiuming, Qiao, Jinzeng, Wang, Jianfang, Cui, Defeng, Gu, Kewei, Zhou, Shuanghai, Li, Huanrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909562/
https://www.ncbi.nlm.nih.gov/pubmed/31831027
http://dx.doi.org/10.1186/s12985-019-1256-z
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author Liu, Shiyu
Li, Qiuming
Qiao, Jinzeng
Wang, Jianfang
Cui, Defeng
Gu, Kewei
Zhou, Shuanghai
Li, Huanrong
author_facet Liu, Shiyu
Li, Qiuming
Qiao, Jinzeng
Wang, Jianfang
Cui, Defeng
Gu, Kewei
Zhou, Shuanghai
Li, Huanrong
author_sort Liu, Shiyu
collection PubMed
description BACKGROUND: Porcine circovirus (PCV) disease caused by PCV type 2 (PCV2) is mainly attributed to immunosuppression and immune damage. PCV2 can infect vascular endothelial cells and induce high expression of endothelial IL-8. Dendritic cells (DCs), as professional antigen-presenting cells, can not only present antigens but also activate naïve T-cells, causing an immune response. METHODS: To demonstrate whether endothelial IL-8 is the main factor inhibiting the maturation and related functions of dendritic cells during PCV2 infection, monocyte-derived DCs (MoDCs) and porcine iliac artery endothelial cells (PIECs) processed by different methods were co-cultured in two ways. Flow cytometry, molecular probe labeling, fluorescence quantitative PCR, and the MTS assay were used to detect the changes in related functions and molecules of MoDCs. RESULTS: Compared to those in the PIEC-DC group, the endothelial IL-8 upregulation co-culture group showed significantly lower double-positive rates for CD80/86 and MHC-II of MoDCs and significantly increased endocytosis of MoDCs. Meanwhile, the adhesion rate and average fluorescence intensity of MoDCs were significantly downregulated in migration and adhesion experiments. Furthermore, the MHC-I and LAMP7 mRNA levels in MoDCs and the proliferation of MoDC-stimulated T-cells were markedly reduced. However, the changes in MoDCs of the endothelial IL-8 downregulation co-culture group were the opposite. CONCLUSIONS: PCV2-induced endothelial IL-8 reduces the adhesion and migration ability of MoDCs, resulting in a decreased maturation rate of MoDCs, and further inhibits antigen presentation by DCs. These results may explain the immunosuppressive mechanism of PCV2 from the perspective of the interaction between endothelial cells and DCs in vitro.
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spelling pubmed-69095622019-12-19 Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function Liu, Shiyu Li, Qiuming Qiao, Jinzeng Wang, Jianfang Cui, Defeng Gu, Kewei Zhou, Shuanghai Li, Huanrong Virol J Research BACKGROUND: Porcine circovirus (PCV) disease caused by PCV type 2 (PCV2) is mainly attributed to immunosuppression and immune damage. PCV2 can infect vascular endothelial cells and induce high expression of endothelial IL-8. Dendritic cells (DCs), as professional antigen-presenting cells, can not only present antigens but also activate naïve T-cells, causing an immune response. METHODS: To demonstrate whether endothelial IL-8 is the main factor inhibiting the maturation and related functions of dendritic cells during PCV2 infection, monocyte-derived DCs (MoDCs) and porcine iliac artery endothelial cells (PIECs) processed by different methods were co-cultured in two ways. Flow cytometry, molecular probe labeling, fluorescence quantitative PCR, and the MTS assay were used to detect the changes in related functions and molecules of MoDCs. RESULTS: Compared to those in the PIEC-DC group, the endothelial IL-8 upregulation co-culture group showed significantly lower double-positive rates for CD80/86 and MHC-II of MoDCs and significantly increased endocytosis of MoDCs. Meanwhile, the adhesion rate and average fluorescence intensity of MoDCs were significantly downregulated in migration and adhesion experiments. Furthermore, the MHC-I and LAMP7 mRNA levels in MoDCs and the proliferation of MoDC-stimulated T-cells were markedly reduced. However, the changes in MoDCs of the endothelial IL-8 downregulation co-culture group were the opposite. CONCLUSIONS: PCV2-induced endothelial IL-8 reduces the adhesion and migration ability of MoDCs, resulting in a decreased maturation rate of MoDCs, and further inhibits antigen presentation by DCs. These results may explain the immunosuppressive mechanism of PCV2 from the perspective of the interaction between endothelial cells and DCs in vitro. BioMed Central 2019-12-12 /pmc/articles/PMC6909562/ /pubmed/31831027 http://dx.doi.org/10.1186/s12985-019-1256-z Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liu, Shiyu
Li, Qiuming
Qiao, Jinzeng
Wang, Jianfang
Cui, Defeng
Gu, Kewei
Zhou, Shuanghai
Li, Huanrong
Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title_full Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title_fullStr Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title_full_unstemmed Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title_short Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
title_sort endothelial il-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909562/
https://www.ncbi.nlm.nih.gov/pubmed/31831027
http://dx.doi.org/10.1186/s12985-019-1256-z
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