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miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer
Gastric cancer (GC) is one of the most aggressive types of human tumor worldwide, and the 5-year survival rate is less than 25%. The transcriptional factor, forkhead box O3 (FOXO3), is regulated by various micro (mi)RNAs and has been reported to be associated with multiple regulatory signaling pathw...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909679/ https://www.ncbi.nlm.nih.gov/pubmed/31853302 http://dx.doi.org/10.3892/etm.2019.8168 |
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author | Li, Ming Wang, Yingxin Liu, Xia Zhang, Zhenduo Wang, Liwei Li, Yong |
author_facet | Li, Ming Wang, Yingxin Liu, Xia Zhang, Zhenduo Wang, Liwei Li, Yong |
author_sort | Li, Ming |
collection | PubMed |
description | Gastric cancer (GC) is one of the most aggressive types of human tumor worldwide, and the 5-year survival rate is less than 25%. The transcriptional factor, forkhead box O3 (FOXO3), is regulated by various micro (mi)RNAs and has been reported to be associated with multiple regulatory signaling pathways involved in tumor development. The current study therefore assessed the impact of miR-629 and FOXO3 on gastric cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to assess the expression of mRNA and protein, respectively. Additionally, the cell proliferation and apoptosis rate were determined via an MTT assay and flow cytometry, respectively. The online database TargetScan predicted that FOXO3 was a target of miR-629. A luciferase reporter assay was also performed to verify that FOXO3 was the direct target of miR-629. The results demonstrated that miR-629 and FOXO3 was upregulated and downregulated in GC tissue, respectively. Furthermore, following transfection with a miR-629 inhibitor, SGC-7901, cell proliferation and apoptosis rate were inhibited and promoted when compared with the control group, respectively. Moreover, after the treatment with SGC-7901, the expression of FOXO3, Bax, Caspase 3 was upregulated, and Bcl-2 was downregulated. Furthermore, the luciferase reporter assay revealed that FOXO3 was the target of miR-629. The results demonstrated that miR-629 and FOXO3 serve vital roles in the development of gastric cancer and may be a future therapeutic target. |
format | Online Article Text |
id | pubmed-6909679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-69096792019-12-18 miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer Li, Ming Wang, Yingxin Liu, Xia Zhang, Zhenduo Wang, Liwei Li, Yong Exp Ther Med Articles Gastric cancer (GC) is one of the most aggressive types of human tumor worldwide, and the 5-year survival rate is less than 25%. The transcriptional factor, forkhead box O3 (FOXO3), is regulated by various micro (mi)RNAs and has been reported to be associated with multiple regulatory signaling pathways involved in tumor development. The current study therefore assessed the impact of miR-629 and FOXO3 on gastric cancer. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to assess the expression of mRNA and protein, respectively. Additionally, the cell proliferation and apoptosis rate were determined via an MTT assay and flow cytometry, respectively. The online database TargetScan predicted that FOXO3 was a target of miR-629. A luciferase reporter assay was also performed to verify that FOXO3 was the direct target of miR-629. The results demonstrated that miR-629 and FOXO3 was upregulated and downregulated in GC tissue, respectively. Furthermore, following transfection with a miR-629 inhibitor, SGC-7901, cell proliferation and apoptosis rate were inhibited and promoted when compared with the control group, respectively. Moreover, after the treatment with SGC-7901, the expression of FOXO3, Bax, Caspase 3 was upregulated, and Bcl-2 was downregulated. Furthermore, the luciferase reporter assay revealed that FOXO3 was the target of miR-629. The results demonstrated that miR-629 and FOXO3 serve vital roles in the development of gastric cancer and may be a future therapeutic target. D.A. Spandidos 2020-01 2019-11-06 /pmc/articles/PMC6909679/ /pubmed/31853302 http://dx.doi.org/10.3892/etm.2019.8168 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Ming Wang, Yingxin Liu, Xia Zhang, Zhenduo Wang, Liwei Li, Yong miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title | miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title_full | miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title_fullStr | miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title_full_unstemmed | miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title_short | miR-629 targets FOXO3 to promote cell apoptosis in gastric cancer |
title_sort | mir-629 targets foxo3 to promote cell apoptosis in gastric cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6909679/ https://www.ncbi.nlm.nih.gov/pubmed/31853302 http://dx.doi.org/10.3892/etm.2019.8168 |
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