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Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea
BACKGROUND AND PURPOSE: Aspergillus fumigatus is one of the most common opportunistic fungus, which causes infection in immunocompromised and neutropenic patients. The current guidelines recommend voriconazole as the initial therapeutic and prophylactic agent for almost all cases, especially in pati...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Society of Medical Mycology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910712/ https://www.ncbi.nlm.nih.gov/pubmed/31850395 http://dx.doi.org/10.18502/cmm.5.3.1745 |
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author | Nazemi, Ladan Hashemi, Seyed Jamal Daie Ghazvini, Roshanak Saeedi, Mina Khodavaisy, Sadegh Barac, Aleksandra Modiri, Mona Akbari Dana, Maryam Zare shahrabadi, Zahra Rezaie, Sassan |
author_facet | Nazemi, Ladan Hashemi, Seyed Jamal Daie Ghazvini, Roshanak Saeedi, Mina Khodavaisy, Sadegh Barac, Aleksandra Modiri, Mona Akbari Dana, Maryam Zare shahrabadi, Zahra Rezaie, Sassan |
author_sort | Nazemi, Ladan |
collection | PubMed |
description | BACKGROUND AND PURPOSE: Aspergillus fumigatus is one of the most common opportunistic fungus, which causes infection in immunocompromised and neutropenic patients. The current guidelines recommend voriconazole as the initial therapeutic and prophylactic agent for almost all cases, especially in patients with organ transplants, which leads to increased medication resistance in A. fumigatus. The aim of the present study was to evaluate the antifungal activity and effect of kombucha as a natural compound on A. fumigatus growth, as well as on the expression of cgrA and cyp51A genes. MATERIALS AND METHODS: A panel of 15 A. fumigatus strains with two quality controls of CM237 and CM2627 as susceptible and resistant strains were obtained from Tehran Medical Mycology Laboratory, Tehran,Iran(TMML).Antifungal susceptibility testing assay was performed according to the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. Moreover, the mycelial dry weight of the fungus was calculated before and after being treated with kombucha. In addition, the quantitative changes in the expression of cgrA and cyp51A genes were analyzed by real-time polymerase chain reaction (real-time PCR) technique. RESULTS: In the present study, the minimum inhibitory concentration ranges of kombucha were measured at 6,170 and 12,300 μg/mL for ten A. fumigatus azole-susceptible strains and 24,700 μg/mL for five A. fumigatus resistant strains. Moreover, changes in mycelial dry weight under kombucha treatment conditions underwent a significant reduction (P≤0.05). A coordinate down-regulation of expression in cgrA and cyp51A genes was observed in all azole-susceptible and -resistant A. fumigatus strains, after treating the fungus with different concentrations of kombucha (P≤0.05). CONCLUSION: According to the obtained results, kombucha as a natural antioxidant , can exert inhibitory effects against the growth and expression of some genes in A. fumigatusstrains. |
format | Online Article Text |
id | pubmed-6910712 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Iranian Society of Medical Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-69107122019-12-17 Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea Nazemi, Ladan Hashemi, Seyed Jamal Daie Ghazvini, Roshanak Saeedi, Mina Khodavaisy, Sadegh Barac, Aleksandra Modiri, Mona Akbari Dana, Maryam Zare shahrabadi, Zahra Rezaie, Sassan Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Aspergillus fumigatus is one of the most common opportunistic fungus, which causes infection in immunocompromised and neutropenic patients. The current guidelines recommend voriconazole as the initial therapeutic and prophylactic agent for almost all cases, especially in patients with organ transplants, which leads to increased medication resistance in A. fumigatus. The aim of the present study was to evaluate the antifungal activity and effect of kombucha as a natural compound on A. fumigatus growth, as well as on the expression of cgrA and cyp51A genes. MATERIALS AND METHODS: A panel of 15 A. fumigatus strains with two quality controls of CM237 and CM2627 as susceptible and resistant strains were obtained from Tehran Medical Mycology Laboratory, Tehran,Iran(TMML).Antifungal susceptibility testing assay was performed according to the Clinical and Laboratory Standards Institute (CLSI) M38-A2 document. Moreover, the mycelial dry weight of the fungus was calculated before and after being treated with kombucha. In addition, the quantitative changes in the expression of cgrA and cyp51A genes were analyzed by real-time polymerase chain reaction (real-time PCR) technique. RESULTS: In the present study, the minimum inhibitory concentration ranges of kombucha were measured at 6,170 and 12,300 μg/mL for ten A. fumigatus azole-susceptible strains and 24,700 μg/mL for five A. fumigatus resistant strains. Moreover, changes in mycelial dry weight under kombucha treatment conditions underwent a significant reduction (P≤0.05). A coordinate down-regulation of expression in cgrA and cyp51A genes was observed in all azole-susceptible and -resistant A. fumigatus strains, after treating the fungus with different concentrations of kombucha (P≤0.05). CONCLUSION: According to the obtained results, kombucha as a natural antioxidant , can exert inhibitory effects against the growth and expression of some genes in A. fumigatusstrains. Iranian Society of Medical Mycology 2019-09 /pmc/articles/PMC6910712/ /pubmed/31850395 http://dx.doi.org/10.18502/cmm.5.3.1745 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Nazemi, Ladan Hashemi, Seyed Jamal Daie Ghazvini, Roshanak Saeedi, Mina Khodavaisy, Sadegh Barac, Aleksandra Modiri, Mona Akbari Dana, Maryam Zare shahrabadi, Zahra Rezaie, Sassan Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title | Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title_full | Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title_fullStr | Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title_full_unstemmed | Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title_short | Investigation of cgrA and cyp51A gene alternations in Aspergillus fumigatus strains exposed to kombucha fermented tea |
title_sort | investigation of cgra and cyp51a gene alternations in aspergillus fumigatus strains exposed to kombucha fermented tea |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910712/ https://www.ncbi.nlm.nih.gov/pubmed/31850395 http://dx.doi.org/10.18502/cmm.5.3.1745 |
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