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MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4
PURPOSE: miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. METHODS: Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-lu...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910861/ https://www.ncbi.nlm.nih.gov/pubmed/31849480 http://dx.doi.org/10.2147/OTT.S210059 |
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author | Chen, Yani Du, Juan Wang, Yu Shi, Haiyan Jiang, Qiuyu Wang, Yangfeng Zhang, Huahua Wei, Yameng Xue, Wanjuan Pu, Zhiying Gao, Yi Li, Dan Feng, Yun Yan, Jing Zhang, Jing |
author_facet | Chen, Yani Du, Juan Wang, Yu Shi, Haiyan Jiang, Qiuyu Wang, Yangfeng Zhang, Huahua Wei, Yameng Xue, Wanjuan Pu, Zhiying Gao, Yi Li, Dan Feng, Yun Yan, Jing Zhang, Jing |
author_sort | Chen, Yani |
collection | PubMed |
description | PURPOSE: miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. METHODS: Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3ʹ-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. RESULTS: Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3ʹ-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. CONCLUSION: All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4. |
format | Online Article Text |
id | pubmed-6910861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69108612019-12-17 MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 Chen, Yani Du, Juan Wang, Yu Shi, Haiyan Jiang, Qiuyu Wang, Yangfeng Zhang, Huahua Wei, Yameng Xue, Wanjuan Pu, Zhiying Gao, Yi Li, Dan Feng, Yun Yan, Jing Zhang, Jing Onco Targets Ther Original Research PURPOSE: miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. METHODS: Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3ʹ-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. RESULTS: Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3ʹ-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. CONCLUSION: All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4. Dove 2019-12-02 /pmc/articles/PMC6910861/ /pubmed/31849480 http://dx.doi.org/10.2147/OTT.S210059 Text en © 2019 Chen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Chen, Yani Du, Juan Wang, Yu Shi, Haiyan Jiang, Qiuyu Wang, Yangfeng Zhang, Huahua Wei, Yameng Xue, Wanjuan Pu, Zhiying Gao, Yi Li, Dan Feng, Yun Yan, Jing Zhang, Jing MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title | MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title_full | MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title_fullStr | MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title_full_unstemmed | MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title_short | MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4 |
title_sort | microrna-497-5p induces cell cycle arrest of cervical cancer cells in s phase by targeting cbx4 |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910861/ https://www.ncbi.nlm.nih.gov/pubmed/31849480 http://dx.doi.org/10.2147/OTT.S210059 |
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