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Does dysbiotic endometrium affect blastocyst implantation in IVF patients?

PURPOSE: To analyze the pregnancy outcomes of IVF patients presenting eubiotic or dysbiotic endometrium at the time of embryo transfer and to analyze what bacterial profiles are suitable for embryo implantation. METHODS: Ninety-nine IVF patients under 40 years old undergoing vitrified-warmed blastoc...

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Detalles Bibliográficos
Autores principales: Hashimoto, Tomoko, Kyono, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910901/
https://www.ncbi.nlm.nih.gov/pubmed/31741256
http://dx.doi.org/10.1007/s10815-019-01630-7
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author Hashimoto, Tomoko
Kyono, Koichi
author_facet Hashimoto, Tomoko
Kyono, Koichi
author_sort Hashimoto, Tomoko
collection PubMed
description PURPOSE: To analyze the pregnancy outcomes of IVF patients presenting eubiotic or dysbiotic endometrium at the time of embryo transfer and to analyze what bacterial profiles are suitable for embryo implantation. METHODS: Ninety-nine IVF patients under 40 years old undergoing vitrified-warmed blastocyst transfer in HRT cycle had concurrent endometrial microbiome analysis. Samples from the endometrium were taken from the participants at the time of mock transfer; the bacterial profiles at genus level and percentage of lactobacilli in the endometrium of the patients were analyzed. RESULTS: Thirty-one cases (31.3%) had dysbiotic endometrium. The background profiles, pregnancy rates per transfer (52.9% vs 54.8%), and miscarriage rates (11.1% vs 5.9%) were comparable between patients with eubiotic or dysbiotic endometrium. Major bacterial genera other than Lactobacillus detected in the dysbiotic endometrium were Atopobium, Gardnerella, and Streptococcus. Some patients achieved ongoing pregnancies with 0% Lactobacillus in the endometrium. The endometrial bacterial profiles of pregnant cases with dysbiotic endometrium were comparable with those of non-pregnant cases. CONCLUSION: Analyzing microbiota at the species-level resolution may be necessary for identifying the true pathogenic bacteria of the endometrium and avoiding over-intervention against non-Lactobacillus microbiota. Further studies are necessary for analyzing the mechanism of how the pathogenic bacteria affect embryo implantation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-019-01630-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-69109012019-12-26 Does dysbiotic endometrium affect blastocyst implantation in IVF patients? Hashimoto, Tomoko Kyono, Koichi J Assist Reprod Genet Assisted Reproduction Technologies PURPOSE: To analyze the pregnancy outcomes of IVF patients presenting eubiotic or dysbiotic endometrium at the time of embryo transfer and to analyze what bacterial profiles are suitable for embryo implantation. METHODS: Ninety-nine IVF patients under 40 years old undergoing vitrified-warmed blastocyst transfer in HRT cycle had concurrent endometrial microbiome analysis. Samples from the endometrium were taken from the participants at the time of mock transfer; the bacterial profiles at genus level and percentage of lactobacilli in the endometrium of the patients were analyzed. RESULTS: Thirty-one cases (31.3%) had dysbiotic endometrium. The background profiles, pregnancy rates per transfer (52.9% vs 54.8%), and miscarriage rates (11.1% vs 5.9%) were comparable between patients with eubiotic or dysbiotic endometrium. Major bacterial genera other than Lactobacillus detected in the dysbiotic endometrium were Atopobium, Gardnerella, and Streptococcus. Some patients achieved ongoing pregnancies with 0% Lactobacillus in the endometrium. The endometrial bacterial profiles of pregnant cases with dysbiotic endometrium were comparable with those of non-pregnant cases. CONCLUSION: Analyzing microbiota at the species-level resolution may be necessary for identifying the true pathogenic bacteria of the endometrium and avoiding over-intervention against non-Lactobacillus microbiota. Further studies are necessary for analyzing the mechanism of how the pathogenic bacteria affect embryo implantation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-019-01630-7) contains supplementary material, which is available to authorized users. Springer US 2019-11-18 2019-12 /pmc/articles/PMC6910901/ /pubmed/31741256 http://dx.doi.org/10.1007/s10815-019-01630-7 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Assisted Reproduction Technologies
Hashimoto, Tomoko
Kyono, Koichi
Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title_full Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title_fullStr Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title_full_unstemmed Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title_short Does dysbiotic endometrium affect blastocyst implantation in IVF patients?
title_sort does dysbiotic endometrium affect blastocyst implantation in ivf patients?
topic Assisted Reproduction Technologies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6910901/
https://www.ncbi.nlm.nih.gov/pubmed/31741256
http://dx.doi.org/10.1007/s10815-019-01630-7
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