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Altered microRNA and target gene expression related to Tetralogy of Fallot

MicroRNAs (miRNAs) play an important role in guiding development and maintaining function of the human heart. Dysregulation of miRNAs has been linked to various congenital heart diseases including Tetralogy of Fallot (TOF), which represents the most common cyanotic heart malformation in humans. Seve...

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Autores principales: Grunert, Marcel, Appelt, Sandra, Dunkel, Ilona, Berger, Felix, Sperling, Silke R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911057/
https://www.ncbi.nlm.nih.gov/pubmed/31836860
http://dx.doi.org/10.1038/s41598-019-55570-4
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author Grunert, Marcel
Appelt, Sandra
Dunkel, Ilona
Berger, Felix
Sperling, Silke R.
author_facet Grunert, Marcel
Appelt, Sandra
Dunkel, Ilona
Berger, Felix
Sperling, Silke R.
author_sort Grunert, Marcel
collection PubMed
description MicroRNAs (miRNAs) play an important role in guiding development and maintaining function of the human heart. Dysregulation of miRNAs has been linked to various congenital heart diseases including Tetralogy of Fallot (TOF), which represents the most common cyanotic heart malformation in humans. Several studies have identified dysregulated miRNAs in right ventricular (RV) tissues of TOF patients. In this study, we profiled genome-wide the whole transcriptome and analyzed the relationship of miRNAs and mRNAs of RV tissues of a homogeneous group of 22 non-syndromic TOF patients. Observed profiles were compared to profiles obtained from right and left ventricular tissue of normal hearts. To reduce the commonly observed large list of predicted target genes of dysregulated miRNAs, we applied a stringent target prediction pipeline integrating probabilities for miRNA-mRNA interaction. The final list of disease-related miRNA-mRNA pairs comprises novel as well as known miRNAs including miR-1 and miR-133, which are essential to cardiac development and function by regulating KCNJ2, FBN2, SLC38A3 and TNNI1. Overall, our study provides additional insights into post-transcriptional gene regulation of malformed hearts of TOF patients.
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spelling pubmed-69110572019-12-16 Altered microRNA and target gene expression related to Tetralogy of Fallot Grunert, Marcel Appelt, Sandra Dunkel, Ilona Berger, Felix Sperling, Silke R. Sci Rep Article MicroRNAs (miRNAs) play an important role in guiding development and maintaining function of the human heart. Dysregulation of miRNAs has been linked to various congenital heart diseases including Tetralogy of Fallot (TOF), which represents the most common cyanotic heart malformation in humans. Several studies have identified dysregulated miRNAs in right ventricular (RV) tissues of TOF patients. In this study, we profiled genome-wide the whole transcriptome and analyzed the relationship of miRNAs and mRNAs of RV tissues of a homogeneous group of 22 non-syndromic TOF patients. Observed profiles were compared to profiles obtained from right and left ventricular tissue of normal hearts. To reduce the commonly observed large list of predicted target genes of dysregulated miRNAs, we applied a stringent target prediction pipeline integrating probabilities for miRNA-mRNA interaction. The final list of disease-related miRNA-mRNA pairs comprises novel as well as known miRNAs including miR-1 and miR-133, which are essential to cardiac development and function by regulating KCNJ2, FBN2, SLC38A3 and TNNI1. Overall, our study provides additional insights into post-transcriptional gene regulation of malformed hearts of TOF patients. Nature Publishing Group UK 2019-12-13 /pmc/articles/PMC6911057/ /pubmed/31836860 http://dx.doi.org/10.1038/s41598-019-55570-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Grunert, Marcel
Appelt, Sandra
Dunkel, Ilona
Berger, Felix
Sperling, Silke R.
Altered microRNA and target gene expression related to Tetralogy of Fallot
title Altered microRNA and target gene expression related to Tetralogy of Fallot
title_full Altered microRNA and target gene expression related to Tetralogy of Fallot
title_fullStr Altered microRNA and target gene expression related to Tetralogy of Fallot
title_full_unstemmed Altered microRNA and target gene expression related to Tetralogy of Fallot
title_short Altered microRNA and target gene expression related to Tetralogy of Fallot
title_sort altered microrna and target gene expression related to tetralogy of fallot
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911057/
https://www.ncbi.nlm.nih.gov/pubmed/31836860
http://dx.doi.org/10.1038/s41598-019-55570-4
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