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MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer
Colorectal cancer (CRC) remains the candidate for one of the typical types of malignant tumors of in gastrointestinal tract all around the world, which leads to tremendous death and ranks as the top leading death of cancer. Recently, microRNAs have emerged as double-edged sword in numerous cancers....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911158/ https://www.ncbi.nlm.nih.gov/pubmed/31729531 http://dx.doi.org/10.1042/BSR20190976 |
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author | Li, Wen-Cui Wu, Yan-Qiong Gao, Bo Wang, Chao-Yun Zhang, Juan-Juan |
author_facet | Li, Wen-Cui Wu, Yan-Qiong Gao, Bo Wang, Chao-Yun Zhang, Juan-Juan |
author_sort | Li, Wen-Cui |
collection | PubMed |
description | Colorectal cancer (CRC) remains the candidate for one of the typical types of malignant tumors of in gastrointestinal tract all around the world, which leads to tremendous death and ranks as the top leading death of cancer. Recently, microRNAs have emerged as double-edged sword in numerous cancers. This investigation aims to discuss the regulative role of microRNA-574-3p (miR-574-3p), elucidating its molecular mechanism and clinical significance in CRC. Herein, it revealed to us that miR-574-3p was lowly expressed in CRC tissues in comparison with the matched paracarcinoma tissues. In addition, transfection of SW480 and HT29 cells with miR-574-3p mimics prohibited the post-transcriptional expression of Cyclin D2 (CCND2), which then significantly blocked cell growth and cell migration, yet triggered cell apoptosis. Also, dual-luciferase reporter assays proved the role of CCND2 as the targeted gene for miR-574-3p. miR-574-3p overexpression prohibited the activity of CCND2 in SW480 and HT29 cells. Silencing of CCND2 in SW480 and HT29 CRC cell lines leading to reduced cell proliferative and migrative rates, and enhanced apoptotic rate. The suppressive effects of elevation of miR-574-3p on the proliferation of the human CRC cells and promotive effects on cell apoptosis by targeting CCND2 were further illustrated in the in vitro studies. Thus, we hypothesize that miR-574-3p may be served as a prospective therapeutic candidate for CRC. |
format | Online Article Text |
id | pubmed-6911158 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69111582019-12-27 MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer Li, Wen-Cui Wu, Yan-Qiong Gao, Bo Wang, Chao-Yun Zhang, Juan-Juan Biosci Rep Cancer Colorectal cancer (CRC) remains the candidate for one of the typical types of malignant tumors of in gastrointestinal tract all around the world, which leads to tremendous death and ranks as the top leading death of cancer. Recently, microRNAs have emerged as double-edged sword in numerous cancers. This investigation aims to discuss the regulative role of microRNA-574-3p (miR-574-3p), elucidating its molecular mechanism and clinical significance in CRC. Herein, it revealed to us that miR-574-3p was lowly expressed in CRC tissues in comparison with the matched paracarcinoma tissues. In addition, transfection of SW480 and HT29 cells with miR-574-3p mimics prohibited the post-transcriptional expression of Cyclin D2 (CCND2), which then significantly blocked cell growth and cell migration, yet triggered cell apoptosis. Also, dual-luciferase reporter assays proved the role of CCND2 as the targeted gene for miR-574-3p. miR-574-3p overexpression prohibited the activity of CCND2 in SW480 and HT29 cells. Silencing of CCND2 in SW480 and HT29 CRC cell lines leading to reduced cell proliferative and migrative rates, and enhanced apoptotic rate. The suppressive effects of elevation of miR-574-3p on the proliferation of the human CRC cells and promotive effects on cell apoptosis by targeting CCND2 were further illustrated in the in vitro studies. Thus, we hypothesize that miR-574-3p may be served as a prospective therapeutic candidate for CRC. Portland Press Ltd. 2019-12-13 /pmc/articles/PMC6911158/ /pubmed/31729531 http://dx.doi.org/10.1042/BSR20190976 Text en © 2019 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
spellingShingle | Cancer Li, Wen-Cui Wu, Yan-Qiong Gao, Bo Wang, Chao-Yun Zhang, Juan-Juan MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title | MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title_full | MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title_fullStr | MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title_full_unstemmed | MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title_short | MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer |
title_sort | mirna-574-3p inhibits cell progression by directly targeting ccnd2 in colorectal cancer |
topic | Cancer |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911158/ https://www.ncbi.nlm.nih.gov/pubmed/31729531 http://dx.doi.org/10.1042/BSR20190976 |
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