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A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression

BACKGROUND: Osteosarcoma (OS) is a quite malignant bone cancer. However, how long noncoding RNA (lncRNA) regulates OS progression remains poorly investigated. The present study aims to illustrate the potential functions of lncRNA MYOSLID in the regulation of OS progression. METHODS: The expression o...

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Autores principales: Yang, Shouhang, Chen, Ming, Lin, Chuanfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911319/
https://www.ncbi.nlm.nih.gov/pubmed/31849524
http://dx.doi.org/10.2147/CMAR.S231376
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author Yang, Shouhang
Chen, Ming
Lin, Chuanfu
author_facet Yang, Shouhang
Chen, Ming
Lin, Chuanfu
author_sort Yang, Shouhang
collection PubMed
description BACKGROUND: Osteosarcoma (OS) is a quite malignant bone cancer. However, how long noncoding RNA (lncRNA) regulates OS progression remains poorly investigated. The present study aims to illustrate the potential functions of lncRNA MYOSLID in the regulation of OS progression. METHODS: The expression of YOSLID, miR-1286 and RAB13 was analyzed by qRT-PCR. Cell proliferation was determined via CCK8 and colony formation assays. Transwell assay was used to examine migration and invasion. The luciferase reporter assay, RNA pulldown and RNA immunoprecipitation (RIP) assays were utilized to detect the interactions among MYOSLID, miR-1286 and RAB13. RESULTS: The expression of MYOSLID was upregulated in OS tissues and cell lines. MYOSLID overexpression predicted poor prognosis in OS patients. MYOSLID knockdown suppressed proliferation, migration and invasion of OS cells. MYOSLID was the sponge for miR-1286 and inhibited its expression while miR-1286 targeted RAB13 directly. MYOSLID promoted RAB13 expression via sponging miR-1286. CONCLUSION: Our work demonstrated that the MYOSLID/miR-1286/RAB13 axis is a novel regulatory signaling in promoting OS progression.
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spelling pubmed-69113192019-12-17 A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression Yang, Shouhang Chen, Ming Lin, Chuanfu Cancer Manag Res Original Research BACKGROUND: Osteosarcoma (OS) is a quite malignant bone cancer. However, how long noncoding RNA (lncRNA) regulates OS progression remains poorly investigated. The present study aims to illustrate the potential functions of lncRNA MYOSLID in the regulation of OS progression. METHODS: The expression of YOSLID, miR-1286 and RAB13 was analyzed by qRT-PCR. Cell proliferation was determined via CCK8 and colony formation assays. Transwell assay was used to examine migration and invasion. The luciferase reporter assay, RNA pulldown and RNA immunoprecipitation (RIP) assays were utilized to detect the interactions among MYOSLID, miR-1286 and RAB13. RESULTS: The expression of MYOSLID was upregulated in OS tissues and cell lines. MYOSLID overexpression predicted poor prognosis in OS patients. MYOSLID knockdown suppressed proliferation, migration and invasion of OS cells. MYOSLID was the sponge for miR-1286 and inhibited its expression while miR-1286 targeted RAB13 directly. MYOSLID promoted RAB13 expression via sponging miR-1286. CONCLUSION: Our work demonstrated that the MYOSLID/miR-1286/RAB13 axis is a novel regulatory signaling in promoting OS progression. Dove 2019-12-10 /pmc/articles/PMC6911319/ /pubmed/31849524 http://dx.doi.org/10.2147/CMAR.S231376 Text en © 2019 Yang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yang, Shouhang
Chen, Ming
Lin, Chuanfu
A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title_full A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title_fullStr A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title_full_unstemmed A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title_short A Novel lncRNA MYOSLID/miR-1286/RAB13 Axis Plays a Critical Role in Osteosarcoma Progression
title_sort novel lncrna myoslid/mir-1286/rab13 axis plays a critical role in osteosarcoma progression
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911319/
https://www.ncbi.nlm.nih.gov/pubmed/31849524
http://dx.doi.org/10.2147/CMAR.S231376
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