Cargando…

MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST

INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR;...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Xuning, Jiang, Honggang, Chen, Zhiheng, Lu, Bohao, Zhu, Yi, Mao, Jiayan, Chai, Kequn, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911328/
https://www.ncbi.nlm.nih.gov/pubmed/31849487
http://dx.doi.org/10.2147/OTT.S216147
_version_ 1783479245771636736
author Shen, Xuning
Jiang, Honggang
Chen, Zhiheng
Lu, Bohao
Zhu, Yi
Mao, Jiayan
Chai, Kequn
Chen, Wei
author_facet Shen, Xuning
Jiang, Honggang
Chen, Zhiheng
Lu, Bohao
Zhu, Yi
Mao, Jiayan
Chai, Kequn
Chen, Wei
author_sort Shen, Xuning
collection PubMed
description INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR; Western blotting was used to detect TWIST1 (twist family bHLH transcription factor 1) protein and the epithelial–mesenchymal transition (EMT)-related proteins (E-cadherin, vimentin). Then, we transfected miR-145 mimics or inhibitor into CRC cells and used the wound healing and Transwell invasion assays to investigate their migration and invasive capability, respectively. RESULTS: The miR-145 mimics suppressed CRC cell invasion and migration significantly; in contrast, miR-145 downregulation had the opposite effect. Furthermore, miR-145 regulated TWIST1 levels negatively at transcriptional level. TWIST1 knockdown significantly inhibited the CRC cell migration ability and the number of CRC cells that crossed the Transwell membrane. There was no significant difference in terms of migration and invasive capability after the cells had been transfected with miR-145 mimics or inhibitor plus TWIST1 small interfering RNA (siRNA) as compared to the TWIST1 siRNA–only group. Furthermore, we demonstrate that the inhibition of miR-145 could enhance the capability for lung metastasis in vivo. CONCLUSION: Taken together, these findings indicate that miR-145 acts as a new tumor suppressor by regulating TWIST1 and plays a vital role in the invasive and migration ability of CRC cells.
format Online
Article
Text
id pubmed-6911328
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-69113282019-12-17 MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST Shen, Xuning Jiang, Honggang Chen, Zhiheng Lu, Bohao Zhu, Yi Mao, Jiayan Chai, Kequn Chen, Wei Onco Targets Ther Original Research INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR; Western blotting was used to detect TWIST1 (twist family bHLH transcription factor 1) protein and the epithelial–mesenchymal transition (EMT)-related proteins (E-cadherin, vimentin). Then, we transfected miR-145 mimics or inhibitor into CRC cells and used the wound healing and Transwell invasion assays to investigate their migration and invasive capability, respectively. RESULTS: The miR-145 mimics suppressed CRC cell invasion and migration significantly; in contrast, miR-145 downregulation had the opposite effect. Furthermore, miR-145 regulated TWIST1 levels negatively at transcriptional level. TWIST1 knockdown significantly inhibited the CRC cell migration ability and the number of CRC cells that crossed the Transwell membrane. There was no significant difference in terms of migration and invasive capability after the cells had been transfected with miR-145 mimics or inhibitor plus TWIST1 small interfering RNA (siRNA) as compared to the TWIST1 siRNA–only group. Furthermore, we demonstrate that the inhibition of miR-145 could enhance the capability for lung metastasis in vivo. CONCLUSION: Taken together, these findings indicate that miR-145 acts as a new tumor suppressor by regulating TWIST1 and plays a vital role in the invasive and migration ability of CRC cells. Dove 2019-12-10 /pmc/articles/PMC6911328/ /pubmed/31849487 http://dx.doi.org/10.2147/OTT.S216147 Text en © 2019 Shen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Shen, Xuning
Jiang, Honggang
Chen, Zhiheng
Lu, Bohao
Zhu, Yi
Mao, Jiayan
Chai, Kequn
Chen, Wei
MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title_full MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title_fullStr MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title_full_unstemmed MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title_short MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
title_sort microrna-145 inhibits cell migration and invasion in colorectal cancer by targeting twist
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911328/
https://www.ncbi.nlm.nih.gov/pubmed/31849487
http://dx.doi.org/10.2147/OTT.S216147
work_keys_str_mv AT shenxuning microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT jianghonggang microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT chenzhiheng microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT lubohao microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT zhuyi microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT maojiayan microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT chaikequn microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist
AT chenwei microrna145inhibitscellmigrationandinvasionincolorectalcancerbytargetingtwist