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MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST
INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR;...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911328/ https://www.ncbi.nlm.nih.gov/pubmed/31849487 http://dx.doi.org/10.2147/OTT.S216147 |
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author | Shen, Xuning Jiang, Honggang Chen, Zhiheng Lu, Bohao Zhu, Yi Mao, Jiayan Chai, Kequn Chen, Wei |
author_facet | Shen, Xuning Jiang, Honggang Chen, Zhiheng Lu, Bohao Zhu, Yi Mao, Jiayan Chai, Kequn Chen, Wei |
author_sort | Shen, Xuning |
collection | PubMed |
description | INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR; Western blotting was used to detect TWIST1 (twist family bHLH transcription factor 1) protein and the epithelial–mesenchymal transition (EMT)-related proteins (E-cadherin, vimentin). Then, we transfected miR-145 mimics or inhibitor into CRC cells and used the wound healing and Transwell invasion assays to investigate their migration and invasive capability, respectively. RESULTS: The miR-145 mimics suppressed CRC cell invasion and migration significantly; in contrast, miR-145 downregulation had the opposite effect. Furthermore, miR-145 regulated TWIST1 levels negatively at transcriptional level. TWIST1 knockdown significantly inhibited the CRC cell migration ability and the number of CRC cells that crossed the Transwell membrane. There was no significant difference in terms of migration and invasive capability after the cells had been transfected with miR-145 mimics or inhibitor plus TWIST1 small interfering RNA (siRNA) as compared to the TWIST1 siRNA–only group. Furthermore, we demonstrate that the inhibition of miR-145 could enhance the capability for lung metastasis in vivo. CONCLUSION: Taken together, these findings indicate that miR-145 acts as a new tumor suppressor by regulating TWIST1 and plays a vital role in the invasive and migration ability of CRC cells. |
format | Online Article Text |
id | pubmed-6911328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-69113282019-12-17 MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST Shen, Xuning Jiang, Honggang Chen, Zhiheng Lu, Bohao Zhu, Yi Mao, Jiayan Chai, Kequn Chen, Wei Onco Targets Ther Original Research INTRODUCTION: MicroRNAs function as oncogenes or tumor suppressors in the development of various human cancers. We investigated the effect of microRNA-145 (miR-145) on colorectal cancer (CRC) cell invasion and migration. METHODS: The levels of miR-145 in CRC cells were examined by quantitative PCR; Western blotting was used to detect TWIST1 (twist family bHLH transcription factor 1) protein and the epithelial–mesenchymal transition (EMT)-related proteins (E-cadherin, vimentin). Then, we transfected miR-145 mimics or inhibitor into CRC cells and used the wound healing and Transwell invasion assays to investigate their migration and invasive capability, respectively. RESULTS: The miR-145 mimics suppressed CRC cell invasion and migration significantly; in contrast, miR-145 downregulation had the opposite effect. Furthermore, miR-145 regulated TWIST1 levels negatively at transcriptional level. TWIST1 knockdown significantly inhibited the CRC cell migration ability and the number of CRC cells that crossed the Transwell membrane. There was no significant difference in terms of migration and invasive capability after the cells had been transfected with miR-145 mimics or inhibitor plus TWIST1 small interfering RNA (siRNA) as compared to the TWIST1 siRNA–only group. Furthermore, we demonstrate that the inhibition of miR-145 could enhance the capability for lung metastasis in vivo. CONCLUSION: Taken together, these findings indicate that miR-145 acts as a new tumor suppressor by regulating TWIST1 and plays a vital role in the invasive and migration ability of CRC cells. Dove 2019-12-10 /pmc/articles/PMC6911328/ /pubmed/31849487 http://dx.doi.org/10.2147/OTT.S216147 Text en © 2019 Shen et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Shen, Xuning Jiang, Honggang Chen, Zhiheng Lu, Bohao Zhu, Yi Mao, Jiayan Chai, Kequn Chen, Wei MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title | MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title_full | MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title_fullStr | MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title_full_unstemmed | MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title_short | MicroRNA-145 Inhibits Cell Migration and Invasion in Colorectal Cancer by Targeting TWIST |
title_sort | microrna-145 inhibits cell migration and invasion in colorectal cancer by targeting twist |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911328/ https://www.ncbi.nlm.nih.gov/pubmed/31849487 http://dx.doi.org/10.2147/OTT.S216147 |
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