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Identification of miRNA precursors in the phloem of Cucurbita maxima

Plant development and responses to environmental cues largely depend on mobile signals including microRNAs (miRNAs) required for post-transcriptional silencing of specific genes. Short-range cell-to-cell transport of miRNA in developing tissues and organs is involved in transferring positional infor...

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Autores principales: Tolstyko, Eugeny, Lezzhov, Alexander, Solovyev, Andrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911342/
https://www.ncbi.nlm.nih.gov/pubmed/31844599
http://dx.doi.org/10.7717/peerj.8269
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author Tolstyko, Eugeny
Lezzhov, Alexander
Solovyev, Andrey
author_facet Tolstyko, Eugeny
Lezzhov, Alexander
Solovyev, Andrey
author_sort Tolstyko, Eugeny
collection PubMed
description Plant development and responses to environmental cues largely depend on mobile signals including microRNAs (miRNAs) required for post-transcriptional silencing of specific genes. Short-range cell-to-cell transport of miRNA in developing tissues and organs is involved in transferring positional information essential for determining cell fate. Among other RNA species, miRNAs are found in the phloem sap. Long-distance transport of miRNA via the phloem takes a part in regulation of physiological responses to changing environmental conditions. As shown for regulation of inorganic phosphorus and sulfate homeostasis, mature miRNAs rather than miRNAs precursors are transported in the phloem as signaling molecules. Here, a bioinformatics analysis of transcriptomic data for Cucurbita maxima phloem exudate RNAs was carried out to elucidate whether miRNA precursors could also be present in the phloem. We demonstrated that the phloem transcriptome contained a subset of C. maxima pri-miRNAs that differed from a subset of pri-miRNA sequences abundant in a leaf transcriptome. Differential accumulation of pri-miRNA was confirmed by PCR analysis of C. maxima phloem exudate and leaf RNA samples. Therefore, the presented data indicate that a number of C. maxima pri-miRNAs are selectively recruited to the phloem translocation pathway. This conclusion was validated by inter-species grafting experiments, in which C. maxima pri-miR319a was found to be transported across the graft union via the phloem, confirming the presence of pri-miR319a in sieve elements and showing that phloem miRNA precursors could play a role in long-distance signaling in plants.
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spelling pubmed-69113422019-12-16 Identification of miRNA precursors in the phloem of Cucurbita maxima Tolstyko, Eugeny Lezzhov, Alexander Solovyev, Andrey PeerJ Cell Biology Plant development and responses to environmental cues largely depend on mobile signals including microRNAs (miRNAs) required for post-transcriptional silencing of specific genes. Short-range cell-to-cell transport of miRNA in developing tissues and organs is involved in transferring positional information essential for determining cell fate. Among other RNA species, miRNAs are found in the phloem sap. Long-distance transport of miRNA via the phloem takes a part in regulation of physiological responses to changing environmental conditions. As shown for regulation of inorganic phosphorus and sulfate homeostasis, mature miRNAs rather than miRNAs precursors are transported in the phloem as signaling molecules. Here, a bioinformatics analysis of transcriptomic data for Cucurbita maxima phloem exudate RNAs was carried out to elucidate whether miRNA precursors could also be present in the phloem. We demonstrated that the phloem transcriptome contained a subset of C. maxima pri-miRNAs that differed from a subset of pri-miRNA sequences abundant in a leaf transcriptome. Differential accumulation of pri-miRNA was confirmed by PCR analysis of C. maxima phloem exudate and leaf RNA samples. Therefore, the presented data indicate that a number of C. maxima pri-miRNAs are selectively recruited to the phloem translocation pathway. This conclusion was validated by inter-species grafting experiments, in which C. maxima pri-miR319a was found to be transported across the graft union via the phloem, confirming the presence of pri-miR319a in sieve elements and showing that phloem miRNA precursors could play a role in long-distance signaling in plants. PeerJ Inc. 2019-12-11 /pmc/articles/PMC6911342/ /pubmed/31844599 http://dx.doi.org/10.7717/peerj.8269 Text en © 2019 Tolstyko et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Cell Biology
Tolstyko, Eugeny
Lezzhov, Alexander
Solovyev, Andrey
Identification of miRNA precursors in the phloem of Cucurbita maxima
title Identification of miRNA precursors in the phloem of Cucurbita maxima
title_full Identification of miRNA precursors in the phloem of Cucurbita maxima
title_fullStr Identification of miRNA precursors in the phloem of Cucurbita maxima
title_full_unstemmed Identification of miRNA precursors in the phloem of Cucurbita maxima
title_short Identification of miRNA precursors in the phloem of Cucurbita maxima
title_sort identification of mirna precursors in the phloem of cucurbita maxima
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911342/
https://www.ncbi.nlm.nih.gov/pubmed/31844599
http://dx.doi.org/10.7717/peerj.8269
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