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Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns

Between February 2018 and April 2018, flowers were collected from eight Rosaceae species. Flowers were kept in a freezer at −20 °C for three freezing times (Treatment 1, two months; Treatment 2, four months; Treatment 3, six months). After extracting pollen, in vitro germination was induced in a cul...

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Autores principales: Beltrán, Roberto, Valls, Aina, Cebrián, Nuria, Zornoza, Carlos, García Breijo, Francisco, Reig Armiñana, José, Garmendia, Alfonso, Merle, Hugo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911343/
https://www.ncbi.nlm.nih.gov/pubmed/31844584
http://dx.doi.org/10.7717/peerj.8195
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author Beltrán, Roberto
Valls, Aina
Cebrián, Nuria
Zornoza, Carlos
García Breijo, Francisco
Reig Armiñana, José
Garmendia, Alfonso
Merle, Hugo
author_facet Beltrán, Roberto
Valls, Aina
Cebrián, Nuria
Zornoza, Carlos
García Breijo, Francisco
Reig Armiñana, José
Garmendia, Alfonso
Merle, Hugo
author_sort Beltrán, Roberto
collection PubMed
description Between February 2018 and April 2018, flowers were collected from eight Rosaceae species. Flowers were kept in a freezer at −20 °C for three freezing times (Treatment 1, two months; Treatment 2, four months; Treatment 3, six months). After extracting pollen, in vitro germination was induced in a culture medium and incubated at six different temperatures for 72 h. The percentage of pollen germination, average pollen tube length and maximum pollen tube length were measured. Pollen germination was maximum for all species between 15 °C and 30 °C. Cydonia oblonga, Malus sylvestris, Prunus avium, Prunus domestica, Prunus dulcis, Prunus persica and Pyrus communis obtained 30–52% pollen germination between 15 °C and 20 °C. Prunus cerasifera had 40% pollen germination at 30 °C. All species studied reached the maximum pollen tube length between 10 °C and 25 °C. Germination did not change significantly for any of the species with freezing time, but we found significant differences in the three parameters measured between treatments. The highest germination percentages were obtained in Treatment 2 (four months frozen at −20 °C), while the maximum pollen tube length was reached in Treatment 1 (two months frozen at −20 °C). According to our results, freezing time affected the germination-temperature patterns. This could indicate that studies on the effect of temperature on pollen germination should always be carried out with fresh pollen to obtain more conclusive data.
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spelling pubmed-69113432019-12-16 Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns Beltrán, Roberto Valls, Aina Cebrián, Nuria Zornoza, Carlos García Breijo, Francisco Reig Armiñana, José Garmendia, Alfonso Merle, Hugo PeerJ Agricultural Science Between February 2018 and April 2018, flowers were collected from eight Rosaceae species. Flowers were kept in a freezer at −20 °C for three freezing times (Treatment 1, two months; Treatment 2, four months; Treatment 3, six months). After extracting pollen, in vitro germination was induced in a culture medium and incubated at six different temperatures for 72 h. The percentage of pollen germination, average pollen tube length and maximum pollen tube length were measured. Pollen germination was maximum for all species between 15 °C and 30 °C. Cydonia oblonga, Malus sylvestris, Prunus avium, Prunus domestica, Prunus dulcis, Prunus persica and Pyrus communis obtained 30–52% pollen germination between 15 °C and 20 °C. Prunus cerasifera had 40% pollen germination at 30 °C. All species studied reached the maximum pollen tube length between 10 °C and 25 °C. Germination did not change significantly for any of the species with freezing time, but we found significant differences in the three parameters measured between treatments. The highest germination percentages were obtained in Treatment 2 (four months frozen at −20 °C), while the maximum pollen tube length was reached in Treatment 1 (two months frozen at −20 °C). According to our results, freezing time affected the germination-temperature patterns. This could indicate that studies on the effect of temperature on pollen germination should always be carried out with fresh pollen to obtain more conclusive data. PeerJ Inc. 2019-12-11 /pmc/articles/PMC6911343/ /pubmed/31844584 http://dx.doi.org/10.7717/peerj.8195 Text en ©2019 Beltrán et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Beltrán, Roberto
Valls, Aina
Cebrián, Nuria
Zornoza, Carlos
García Breijo, Francisco
Reig Armiñana, José
Garmendia, Alfonso
Merle, Hugo
Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title_full Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title_fullStr Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title_full_unstemmed Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title_short Effect of temperature on pollen germination for several Rosaceae species: influence of freezing conservation time on germination patterns
title_sort effect of temperature on pollen germination for several rosaceae species: influence of freezing conservation time on germination patterns
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911343/
https://www.ncbi.nlm.nih.gov/pubmed/31844584
http://dx.doi.org/10.7717/peerj.8195
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