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Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil
Blastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity and epidemiology are still controversial. Currently, it has been associated with intestinal diseases such as irritable bowel syndrome and clinical manifestations of allergic skin...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911935/ https://www.ncbi.nlm.nih.gov/pubmed/31872093 http://dx.doi.org/10.1016/j.parepi.2019.e00124 |
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author | Melo, Gessica Baptista de Malta, Fernanda de Mello Maruta, Celina Wakisaka Criado, Paulo Ricardo Castilho, Vera Lucia Pagliusi Gonçalves, Elenice Messias do Nascimento Espirito-Santo, Maria Cristina de Carvalho do Paula, Fabiana Martins de Gryschek, Ronaldo Cesar Borges |
author_facet | Melo, Gessica Baptista de Malta, Fernanda de Mello Maruta, Celina Wakisaka Criado, Paulo Ricardo Castilho, Vera Lucia Pagliusi Gonçalves, Elenice Messias do Nascimento Espirito-Santo, Maria Cristina de Carvalho do Paula, Fabiana Martins de Gryschek, Ronaldo Cesar Borges |
author_sort | Melo, Gessica Baptista de |
collection | PubMed |
description | Blastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity and epidemiology are still controversial. Currently, it has been associated with intestinal diseases such as irritable bowel syndrome and clinical manifestations of allergic skin, such as chronic urticaria. In the context of urticaria, it is still uncertain whether this organism is directly related to the allergic manifestation or just a common component of the intestinal microbiota. This study aimed to evaluate the occurrence and molecular diversity of Blastocystis sp. in individuals with urticaria from a dermatology outpatient clinic, São Paulo, Brazil. Fecal samples of 58 patients with urticaria were examined using parasitological methods; and subsequently tested by polymerase chain reaction using Blastocystis-specific primers. The subtypes (STs) and alleles (a) were determined using BLASTn and MLST tools. ST1, ST2, ST3, ST4, ST6 and mixed infection (ST1 + ST3) were identified in the patients with urticaria; ST1 (a4), ST3 (a34 and a36) and ST4 (a42) were the most prevalent. Our molecular analyses allowed an initial description of Blastocystis subtypes in patients with urticaria from São Paulo city, Brazil. |
format | Online Article Text |
id | pubmed-6911935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69119352019-12-23 Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil Melo, Gessica Baptista de Malta, Fernanda de Mello Maruta, Celina Wakisaka Criado, Paulo Ricardo Castilho, Vera Lucia Pagliusi Gonçalves, Elenice Messias do Nascimento Espirito-Santo, Maria Cristina de Carvalho do Paula, Fabiana Martins de Gryschek, Ronaldo Cesar Borges Parasite Epidemiol Control Special section on Second International Blastocystis Conference Blastocystis sp. is described as an enteric protist prevalent in fecal samples from humans and animals; its pathogenicity and epidemiology are still controversial. Currently, it has been associated with intestinal diseases such as irritable bowel syndrome and clinical manifestations of allergic skin, such as chronic urticaria. In the context of urticaria, it is still uncertain whether this organism is directly related to the allergic manifestation or just a common component of the intestinal microbiota. This study aimed to evaluate the occurrence and molecular diversity of Blastocystis sp. in individuals with urticaria from a dermatology outpatient clinic, São Paulo, Brazil. Fecal samples of 58 patients with urticaria were examined using parasitological methods; and subsequently tested by polymerase chain reaction using Blastocystis-specific primers. The subtypes (STs) and alleles (a) were determined using BLASTn and MLST tools. ST1, ST2, ST3, ST4, ST6 and mixed infection (ST1 + ST3) were identified in the patients with urticaria; ST1 (a4), ST3 (a34 and a36) and ST4 (a42) were the most prevalent. Our molecular analyses allowed an initial description of Blastocystis subtypes in patients with urticaria from São Paulo city, Brazil. Elsevier 2019-11-09 /pmc/articles/PMC6911935/ /pubmed/31872093 http://dx.doi.org/10.1016/j.parepi.2019.e00124 Text en © 2019 The Authors. Published by Elsevier Ltd on behalf of World Federation of Parasitologists. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Special section on Second International Blastocystis Conference Melo, Gessica Baptista de Malta, Fernanda de Mello Maruta, Celina Wakisaka Criado, Paulo Ricardo Castilho, Vera Lucia Pagliusi Gonçalves, Elenice Messias do Nascimento Espirito-Santo, Maria Cristina de Carvalho do Paula, Fabiana Martins de Gryschek, Ronaldo Cesar Borges Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title | Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title_full | Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title_fullStr | Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title_full_unstemmed | Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title_short | Characterization of subtypes of Blastocystis sp. isolated from patients with urticaria, São Paulo, Brazil |
title_sort | characterization of subtypes of blastocystis sp. isolated from patients with urticaria, são paulo, brazil |
topic | Special section on Second International Blastocystis Conference |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911935/ https://www.ncbi.nlm.nih.gov/pubmed/31872093 http://dx.doi.org/10.1016/j.parepi.2019.e00124 |
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