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Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction
BACKGROUND: Both inflammatory and remodelling processes are associated with irreversible airway obstruction observed in severe asthma. Our aim was to characterize a group of severe asthmatic patients with or without persistent airway obstruction in relation to specific sputum inflammatory and remode...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
World Allergy Organization
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911957/ https://www.ncbi.nlm.nih.gov/pubmed/31871533 http://dx.doi.org/10.1016/j.waojou.2019.100078 |
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author | Cianchetti, Silvana Cardini, Cristina Puxeddu, Ilaria Latorre, Manuela Bartoli, Maria Laura Bradicich, Matteo Dente, Federico Bacci, Elena Celi, Alessandro Paggiaro, Pierluigi |
author_facet | Cianchetti, Silvana Cardini, Cristina Puxeddu, Ilaria Latorre, Manuela Bartoli, Maria Laura Bradicich, Matteo Dente, Federico Bacci, Elena Celi, Alessandro Paggiaro, Pierluigi |
author_sort | Cianchetti, Silvana |
collection | PubMed |
description | BACKGROUND: Both inflammatory and remodelling processes are associated with irreversible airway obstruction observed in severe asthma. Our aim was to characterize a group of severe asthmatic patients with or without persistent airway obstruction in relation to specific sputum inflammatory and remodelling biomarkers. METHODS: Forty-five patients under regular high-dose inhaled corticosteroid/ß-2agonist treatment were studied, after a follow-up period of at least 2 years, with a minimum of 4 visits. Periostin, TGF-ß, RANTES, IL-8, GM-CSF, FGF-2, and cell counts were measured in induced sputum. Serum periostin was also measured. RESULTS: Sputum induction was successfully performed in all but 5 patients. There were no significant differences in demographic and clinical data between patients with non-persistent obstruction (NO: FEV(1)/VC>88%pred.) and those with persistent obstruction (O: a not completely reversible obstruction with FEV(1)/VC<88%pred. at each visit before the study visit). Patients with persistent obstruction had significantly higher sputum periostin and TGF-ß concentrations than NO patients and a trend of higher serum periostin levels. GM-CSF and FGF-2 were significantly increased in NO compared to O patients. No differences between groups were found for RANTES, IL-8 and differential cell counts. Sputum periostin inversely correlated with functional parameters (prebronch. FEV(1): rho = −0.36, p < 0.05; postbronch. FEV(1): rho = −0.33, p = 0.05). Patients with high sputum periostin concentration (>103.3 pg/ml: median value) showed an absolute number of sputum eosinophils significantly higher than patients with low sputum periostin; this behavior was unobserved when serum periostin was considered. CONCLUSIONS: Only periostin and TGF-ß identified a subgroup of severe asthmatic patients with persistent airway obstruction. Sputum periostin was also inversely associated with FEV(1) and proved to be a more sensitive biomarker than serum periostin to identify severe asthmatics with higher sputum eosinophilia. |
format | Online Article Text |
id | pubmed-6911957 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | World Allergy Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-69119572019-12-23 Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction Cianchetti, Silvana Cardini, Cristina Puxeddu, Ilaria Latorre, Manuela Bartoli, Maria Laura Bradicich, Matteo Dente, Federico Bacci, Elena Celi, Alessandro Paggiaro, Pierluigi World Allergy Organ J Article BACKGROUND: Both inflammatory and remodelling processes are associated with irreversible airway obstruction observed in severe asthma. Our aim was to characterize a group of severe asthmatic patients with or without persistent airway obstruction in relation to specific sputum inflammatory and remodelling biomarkers. METHODS: Forty-five patients under regular high-dose inhaled corticosteroid/ß-2agonist treatment were studied, after a follow-up period of at least 2 years, with a minimum of 4 visits. Periostin, TGF-ß, RANTES, IL-8, GM-CSF, FGF-2, and cell counts were measured in induced sputum. Serum periostin was also measured. RESULTS: Sputum induction was successfully performed in all but 5 patients. There were no significant differences in demographic and clinical data between patients with non-persistent obstruction (NO: FEV(1)/VC>88%pred.) and those with persistent obstruction (O: a not completely reversible obstruction with FEV(1)/VC<88%pred. at each visit before the study visit). Patients with persistent obstruction had significantly higher sputum periostin and TGF-ß concentrations than NO patients and a trend of higher serum periostin levels. GM-CSF and FGF-2 were significantly increased in NO compared to O patients. No differences between groups were found for RANTES, IL-8 and differential cell counts. Sputum periostin inversely correlated with functional parameters (prebronch. FEV(1): rho = −0.36, p < 0.05; postbronch. FEV(1): rho = −0.33, p = 0.05). Patients with high sputum periostin concentration (>103.3 pg/ml: median value) showed an absolute number of sputum eosinophils significantly higher than patients with low sputum periostin; this behavior was unobserved when serum periostin was considered. CONCLUSIONS: Only periostin and TGF-ß identified a subgroup of severe asthmatic patients with persistent airway obstruction. Sputum periostin was also inversely associated with FEV(1) and proved to be a more sensitive biomarker than serum periostin to identify severe asthmatics with higher sputum eosinophilia. World Allergy Organization 2019-12-09 /pmc/articles/PMC6911957/ /pubmed/31871533 http://dx.doi.org/10.1016/j.waojou.2019.100078 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Cianchetti, Silvana Cardini, Cristina Puxeddu, Ilaria Latorre, Manuela Bartoli, Maria Laura Bradicich, Matteo Dente, Federico Bacci, Elena Celi, Alessandro Paggiaro, Pierluigi Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title | Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title_full | Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title_fullStr | Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title_full_unstemmed | Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title_short | Distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
title_sort | distinct profile of inflammatory and remodelling biomarkers in sputum of severe asthmatic patients with or without persistent airway obstruction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6911957/ https://www.ncbi.nlm.nih.gov/pubmed/31871533 http://dx.doi.org/10.1016/j.waojou.2019.100078 |
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