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Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse

Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, includ...

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Autores principales: Putra, Wira Eka, Rifa'i, Muhaimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912186/
https://www.ncbi.nlm.nih.gov/pubmed/31857966
http://dx.doi.org/10.15171/apb.2019.071
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author Putra, Wira Eka
Rifa'i, Muhaimin
author_facet Putra, Wira Eka
Rifa'i, Muhaimin
author_sort Putra, Wira Eka
collection PubMed
description Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, including the immune system. Therefore, this study extensively aimed to evaluate the immunomodulatory activities of Sambucus javanica extracts in 7,12-dimethylbenz[a] anthracene (DMBA)-treated BALB/c mouse. Methods: The experimental mice were orally administrated with 2.8 mg.kg(-1) BW of DMBA for ten times within a month. After that, the mice were treated by S. javanica berries and leaves extracts for 2 weeks. Subsequently, the inflammation rate was evaluated by using flow cytometry analysis, whereas the necrosis incidences were observed by hematoxylin & eosin staining. Results: Based on the results, we found the expression of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-ɣ) were increased however after treated by S. javanica berries and leaves extracts were significantly decreased. In the same way, necrosis incidence was increased in the DMBA-treated group however it was diminished with S. javanica extracts treatment. Conclusion: Together, these results suggested that S. javanica extracts have immunomodulatory activities to suppress inflammation and reduce necrosis incidence in experimental mice.
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spelling pubmed-69121862019-12-19 Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse Putra, Wira Eka Rifa'i, Muhaimin Adv Pharm Bull Research Article Purpose: Accumulating evidence shows the genus of Sambucus exerts a broad spectrum of medicinal potencies such as anticancer, antiviral, antibacterial, and antidiabetes. Based on the previous studies, we hypothesized that bioactive compounds of Sambucus might alter several biological systems, including the immune system. Therefore, this study extensively aimed to evaluate the immunomodulatory activities of Sambucus javanica extracts in 7,12-dimethylbenz[a] anthracene (DMBA)-treated BALB/c mouse. Methods: The experimental mice were orally administrated with 2.8 mg.kg(-1) BW of DMBA for ten times within a month. After that, the mice were treated by S. javanica berries and leaves extracts for 2 weeks. Subsequently, the inflammation rate was evaluated by using flow cytometry analysis, whereas the necrosis incidences were observed by hematoxylin & eosin staining. Results: Based on the results, we found the expression of tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-ɣ) were increased however after treated by S. javanica berries and leaves extracts were significantly decreased. In the same way, necrosis incidence was increased in the DMBA-treated group however it was diminished with S. javanica extracts treatment. Conclusion: Together, these results suggested that S. javanica extracts have immunomodulatory activities to suppress inflammation and reduce necrosis incidence in experimental mice. Tabriz University of Medical Sciences 2019-10 2019-10-24 /pmc/articles/PMC6912186/ /pubmed/31857966 http://dx.doi.org/10.15171/apb.2019.071 Text en © 2019 The Author (s) http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, as long as the original authors and source are cited. No permission is required from the authors or the publishers.
spellingShingle Research Article
Putra, Wira Eka
Rifa'i, Muhaimin
Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title_full Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title_fullStr Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title_full_unstemmed Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title_short Immunomodulatory Activities of Sambucus javanica Extracts in DMBA-Exposed BALB/c Mouse
title_sort immunomodulatory activities of sambucus javanica extracts in dmba-exposed balb/c mouse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912186/
https://www.ncbi.nlm.nih.gov/pubmed/31857966
http://dx.doi.org/10.15171/apb.2019.071
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