Cargando…

SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis

Centriolar satellites are non-membrane cytoplasmic granules that deliver proteins to centrosome during centrosome biogenesis and ciliogenesis. Centriolar satellites are highly dynamic during cell cycle or ciliogenesis and how they are regulated remains largely unknown. We report here that sorting ne...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Pengtao, Xia, Jianhong, Zhang, Leilei, Zhao, Shaoyang, Li, Shengbiao, Wang, Haiyun, Cheng, Shan, Li, Heying, Yin, Wenguang, Pei, Duanqing, Shu, Xiaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912348/
https://www.ncbi.nlm.nih.gov/pubmed/31671755
http://dx.doi.org/10.3390/cells8111335
_version_ 1783479434826743808
author Wang, Pengtao
Xia, Jianhong
Zhang, Leilei
Zhao, Shaoyang
Li, Shengbiao
Wang, Haiyun
Cheng, Shan
Li, Heying
Yin, Wenguang
Pei, Duanqing
Shu, Xiaodong
author_facet Wang, Pengtao
Xia, Jianhong
Zhang, Leilei
Zhao, Shaoyang
Li, Shengbiao
Wang, Haiyun
Cheng, Shan
Li, Heying
Yin, Wenguang
Pei, Duanqing
Shu, Xiaodong
author_sort Wang, Pengtao
collection PubMed
description Centriolar satellites are non-membrane cytoplasmic granules that deliver proteins to centrosome during centrosome biogenesis and ciliogenesis. Centriolar satellites are highly dynamic during cell cycle or ciliogenesis and how they are regulated remains largely unknown. We report here that sorting nexin 17 (SNX17) regulates the homeostasis of a subset of centriolar satellite proteins including PCM1, CEP131, and OFD1 during serum-starvation-induced ciliogenesis. Mechanistically, SNX17 recruits the deubiquitinating enzyme USP9X to antagonize the mindbomb 1 (MIB1)-induced ubiquitination and degradation of PCM1. SNX17 deficiency leads to enhanced degradation of USP9X as well as PCM1 and disrupts ciliogenesis upon serum starvation. On the other hand, SNX17 is dispensable for the homeostasis of PCM1 and USP9X in serum-containing media. These findings reveal a SNX17/USP9X mediated pathway essential for the homeostasis of centriolar satellites under serum starvation, and provide insight into the mechanism of USP9X in ciliogenesis, which may lead to a better understating of USP9X-deficiency-related human diseases such as X-linked mental retardation and neurodegenerative diseases.
format Online
Article
Text
id pubmed-6912348
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69123482020-01-02 SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis Wang, Pengtao Xia, Jianhong Zhang, Leilei Zhao, Shaoyang Li, Shengbiao Wang, Haiyun Cheng, Shan Li, Heying Yin, Wenguang Pei, Duanqing Shu, Xiaodong Cells Article Centriolar satellites are non-membrane cytoplasmic granules that deliver proteins to centrosome during centrosome biogenesis and ciliogenesis. Centriolar satellites are highly dynamic during cell cycle or ciliogenesis and how they are regulated remains largely unknown. We report here that sorting nexin 17 (SNX17) regulates the homeostasis of a subset of centriolar satellite proteins including PCM1, CEP131, and OFD1 during serum-starvation-induced ciliogenesis. Mechanistically, SNX17 recruits the deubiquitinating enzyme USP9X to antagonize the mindbomb 1 (MIB1)-induced ubiquitination and degradation of PCM1. SNX17 deficiency leads to enhanced degradation of USP9X as well as PCM1 and disrupts ciliogenesis upon serum starvation. On the other hand, SNX17 is dispensable for the homeostasis of PCM1 and USP9X in serum-containing media. These findings reveal a SNX17/USP9X mediated pathway essential for the homeostasis of centriolar satellites under serum starvation, and provide insight into the mechanism of USP9X in ciliogenesis, which may lead to a better understating of USP9X-deficiency-related human diseases such as X-linked mental retardation and neurodegenerative diseases. MDPI 2019-10-29 /pmc/articles/PMC6912348/ /pubmed/31671755 http://dx.doi.org/10.3390/cells8111335 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Pengtao
Xia, Jianhong
Zhang, Leilei
Zhao, Shaoyang
Li, Shengbiao
Wang, Haiyun
Cheng, Shan
Li, Heying
Yin, Wenguang
Pei, Duanqing
Shu, Xiaodong
SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title_full SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title_fullStr SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title_full_unstemmed SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title_short SNX17 Recruits USP9X to Antagonize MIB1-Mediated Ubiquitination and Degradation of PCM1 during Serum-Starvation-Induced Ciliogenesis
title_sort snx17 recruits usp9x to antagonize mib1-mediated ubiquitination and degradation of pcm1 during serum-starvation-induced ciliogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912348/
https://www.ncbi.nlm.nih.gov/pubmed/31671755
http://dx.doi.org/10.3390/cells8111335
work_keys_str_mv AT wangpengtao snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT xiajianhong snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT zhangleilei snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT zhaoshaoyang snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT lishengbiao snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT wanghaiyun snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT chengshan snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT liheying snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT yinwenguang snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT peiduanqing snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis
AT shuxiaodong snx17recruitsusp9xtoantagonizemib1mediatedubiquitinationanddegradationofpcm1duringserumstarvationinducedciliogenesis