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Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma
Salivary IL-6 mRNA was previously identified as a promising biomarker of oral squamous cell carcinoma (OSCC). We performed a multi-center investigation covering all geographic areas of Hungary. Saliva from 95 patients with OSCC and 80 controls, all Caucasian, were collected together with demographic...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912409/ https://www.ncbi.nlm.nih.gov/pubmed/31766212 http://dx.doi.org/10.3390/jcm8111958 |
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author | Márton, Ildikó Judit Horváth, József Lábiscsák, Péter Márkus, Bernadett Dezső, Balázs Szabó, Adrienn Tar, Ildikó Piffkó, József Jakus, Petra Barabás, József Barabás, Péter Olasz, Lajos Kövér, Zsanett Tőzsér, József Sándor, János Csősz, Éva Scholtz, Beáta Kiss, Csongor |
author_facet | Márton, Ildikó Judit Horváth, József Lábiscsák, Péter Márkus, Bernadett Dezső, Balázs Szabó, Adrienn Tar, Ildikó Piffkó, József Jakus, Petra Barabás, József Barabás, Péter Olasz, Lajos Kövér, Zsanett Tőzsér, József Sándor, János Csősz, Éva Scholtz, Beáta Kiss, Csongor |
author_sort | Márton, Ildikó Judit |
collection | PubMed |
description | Salivary IL-6 mRNA was previously identified as a promising biomarker of oral squamous cell carcinoma (OSCC). We performed a multi-center investigation covering all geographic areas of Hungary. Saliva from 95 patients with OSCC and 80 controls, all Caucasian, were collected together with demographic and clinicopathological data. Salivary IL-6 mRNA was quantified by real-time quantitative PCR. Salivary IL-6 protein concentration was measured by enzyme-linked immune-sorbent assay. IL-6 protein expression in tumor samples was investigated by immunohistochemistry. Normalized salivary IL-6 mRNA expression values were significantly higher (p < 0.001) in patients with OSCC (mean ± SE: 3.301 ± 0.885) vs. controls (mean ± SE: 0.037 ± 0.012). Differences remained significant regardless of tumor stage and grade. AUC of the ROC curve was 0.9379 (p < 0.001; 95% confidence interval: 0.8973–0.9795; sensitivity: 0.945; specificity: 0.819). Salivary IL-6 protein levels were significantly higher (p < 0.001) in patients (mean ± SE: 70.98 ± 14.06 pg/mL), than in controls (mean ± SE: 12.45 ± 3.29). Specificity and sensitivity of IL-6 protein were less favorable than that of IL-6 mRNA. Salivary IL-6 mRNA expression was significantly associated with age and dental status. IL-6 manifestation was detected in tumor cells and tumor-infiltrating leukocytes, suggesting the presence of a paracrine loop of stimulation. Salivary IL-6 mRNA is one of the best performing and clinically relevant biomarkers of OSCC. |
format | Online Article Text |
id | pubmed-6912409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69124092020-01-02 Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma Márton, Ildikó Judit Horváth, József Lábiscsák, Péter Márkus, Bernadett Dezső, Balázs Szabó, Adrienn Tar, Ildikó Piffkó, József Jakus, Petra Barabás, József Barabás, Péter Olasz, Lajos Kövér, Zsanett Tőzsér, József Sándor, János Csősz, Éva Scholtz, Beáta Kiss, Csongor J Clin Med Article Salivary IL-6 mRNA was previously identified as a promising biomarker of oral squamous cell carcinoma (OSCC). We performed a multi-center investigation covering all geographic areas of Hungary. Saliva from 95 patients with OSCC and 80 controls, all Caucasian, were collected together with demographic and clinicopathological data. Salivary IL-6 mRNA was quantified by real-time quantitative PCR. Salivary IL-6 protein concentration was measured by enzyme-linked immune-sorbent assay. IL-6 protein expression in tumor samples was investigated by immunohistochemistry. Normalized salivary IL-6 mRNA expression values were significantly higher (p < 0.001) in patients with OSCC (mean ± SE: 3.301 ± 0.885) vs. controls (mean ± SE: 0.037 ± 0.012). Differences remained significant regardless of tumor stage and grade. AUC of the ROC curve was 0.9379 (p < 0.001; 95% confidence interval: 0.8973–0.9795; sensitivity: 0.945; specificity: 0.819). Salivary IL-6 protein levels were significantly higher (p < 0.001) in patients (mean ± SE: 70.98 ± 14.06 pg/mL), than in controls (mean ± SE: 12.45 ± 3.29). Specificity and sensitivity of IL-6 protein were less favorable than that of IL-6 mRNA. Salivary IL-6 mRNA expression was significantly associated with age and dental status. IL-6 manifestation was detected in tumor cells and tumor-infiltrating leukocytes, suggesting the presence of a paracrine loop of stimulation. Salivary IL-6 mRNA is one of the best performing and clinically relevant biomarkers of OSCC. MDPI 2019-11-13 /pmc/articles/PMC6912409/ /pubmed/31766212 http://dx.doi.org/10.3390/jcm8111958 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Márton, Ildikó Judit Horváth, József Lábiscsák, Péter Márkus, Bernadett Dezső, Balázs Szabó, Adrienn Tar, Ildikó Piffkó, József Jakus, Petra Barabás, József Barabás, Péter Olasz, Lajos Kövér, Zsanett Tőzsér, József Sándor, János Csősz, Éva Scholtz, Beáta Kiss, Csongor Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title | Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title_full | Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title_fullStr | Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title_full_unstemmed | Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title_short | Salivary IL-6 mRNA is a Robust Biomarker in Oral Squamous Cell Carcinoma |
title_sort | salivary il-6 mrna is a robust biomarker in oral squamous cell carcinoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912409/ https://www.ncbi.nlm.nih.gov/pubmed/31766212 http://dx.doi.org/10.3390/jcm8111958 |
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