hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us

Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) have been intensively used in drug development and disease modeling. Since iPSC-cardiomyocyte (CM) was first generated, their characterization has become a major focus of research. Multi-/micro-electrode array (MEA) systems provi...

Descripción completa

Detalles Bibliográficos
Autores principales: Kussauer, Sophie, David, Robert, Lemcke, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912416/
https://www.ncbi.nlm.nih.gov/pubmed/31661896
http://dx.doi.org/10.3390/cells8111331
_version_ 1783479450956988416
author Kussauer, Sophie
David, Robert
Lemcke, Heiko
author_facet Kussauer, Sophie
David, Robert
Lemcke, Heiko
author_sort Kussauer, Sophie
collection PubMed
description Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) have been intensively used in drug development and disease modeling. Since iPSC-cardiomyocyte (CM) was first generated, their characterization has become a major focus of research. Multi-/micro-electrode array (MEA) systems provide a non-invasive user-friendly platform for detailed electrophysiological analysis of iPSC cardiomyocytes including drug testing to identify potential targets and the assessment of proarrhythmic risk. Here, we provide a systematical overview about the physiological and technical background of micro-electrode array measurements of iPSC-CM. We introduce the similarities and differences between action- and field potential and the advantages and drawbacks of MEA technology. In addition, we present current studies focusing on proarrhythmic side effects of novel and established compounds combining MEA systems and iPSC-CM. MEA technology will help to open a new gateway for novel therapies in cardiovascular diseases while reducing animal experiments at the same time.
format Online
Article
Text
id pubmed-6912416
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69124162020-01-02 hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us Kussauer, Sophie David, Robert Lemcke, Heiko Cells Review Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CM) have been intensively used in drug development and disease modeling. Since iPSC-cardiomyocyte (CM) was first generated, their characterization has become a major focus of research. Multi-/micro-electrode array (MEA) systems provide a non-invasive user-friendly platform for detailed electrophysiological analysis of iPSC cardiomyocytes including drug testing to identify potential targets and the assessment of proarrhythmic risk. Here, we provide a systematical overview about the physiological and technical background of micro-electrode array measurements of iPSC-CM. We introduce the similarities and differences between action- and field potential and the advantages and drawbacks of MEA technology. In addition, we present current studies focusing on proarrhythmic side effects of novel and established compounds combining MEA systems and iPSC-CM. MEA technology will help to open a new gateway for novel therapies in cardiovascular diseases while reducing animal experiments at the same time. MDPI 2019-10-28 /pmc/articles/PMC6912416/ /pubmed/31661896 http://dx.doi.org/10.3390/cells8111331 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kussauer, Sophie
David, Robert
Lemcke, Heiko
hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title_full hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title_fullStr hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title_full_unstemmed hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title_short hiPSCs Derived Cardiac Cells for Drug and Toxicity Screening and Disease Modeling: What Micro- Electrode-Array Analyses Can Tell Us
title_sort hipscs derived cardiac cells for drug and toxicity screening and disease modeling: what micro- electrode-array analyses can tell us
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912416/
https://www.ncbi.nlm.nih.gov/pubmed/31661896
http://dx.doi.org/10.3390/cells8111331
work_keys_str_mv AT kussauersophie hipscsderivedcardiaccellsfordrugandtoxicityscreeninganddiseasemodelingwhatmicroelectrodearrayanalysescantellus
AT davidrobert hipscsderivedcardiaccellsfordrugandtoxicityscreeninganddiseasemodelingwhatmicroelectrodearrayanalysescantellus
AT lemckeheiko hipscsderivedcardiaccellsfordrugandtoxicityscreeninganddiseasemodelingwhatmicroelectrodearrayanalysescantellus

Ejemplares similares