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Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations
Acetate can be efficiently metabolized by the green microalga Chlamydomonas reinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were c...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912441/ https://www.ncbi.nlm.nih.gov/pubmed/31683711 http://dx.doi.org/10.3390/cells8111367 |
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author | Bogaert, Kenny A. Perez, Emilie Rumin, Judith Giltay, Axel Carone, Michele Coosemans, Nadine Radoux, Michele Eppe, Gauthier Levine, Raphael D. Remacle, Francoise Remacle, Claire |
author_facet | Bogaert, Kenny A. Perez, Emilie Rumin, Judith Giltay, Axel Carone, Michele Coosemans, Nadine Radoux, Michele Eppe, Gauthier Levine, Raphael D. Remacle, Francoise Remacle, Claire |
author_sort | Bogaert, Kenny A. |
collection | PubMed |
description | Acetate can be efficiently metabolized by the green microalga Chlamydomonas reinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were cultivated in batch mode in the light at 17, 31, 44, and 57 mM acetate. Metabolic analyses show that cells grown at 57 mM acetate possess increased contents of all components analyzed (starch, chlorophylls, fatty acids, and proteins), with a three-fold increased volumetric biomass yield compared to cells cultivated at 17 mM acetate at the entry of stationary phase. Physiological analyses highlight the importance of photosynthesis for the low-acetate and exponential-phase samples. The stationary phase is reached when acetate is depleted, except for the cells grown at 57 mM acetate, which still divide until ammonium exhaustion. Surprisal analysis of the transcriptomics data supports the biological significance of our experiments. This allows the establishment of a model for acetate assimilation, its transcriptional regulation and the identification of candidates for genetic engineering of this metabolic pathway. Altogether, our analyses suggest that growing at high-acetate concentrations could increase biomass productivities in low-light and CO(2)-limiting air-bubbled medium for biotechnology. |
format | Online Article Text |
id | pubmed-6912441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69124412020-01-02 Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations Bogaert, Kenny A. Perez, Emilie Rumin, Judith Giltay, Axel Carone, Michele Coosemans, Nadine Radoux, Michele Eppe, Gauthier Levine, Raphael D. Remacle, Francoise Remacle, Claire Cells Article Acetate can be efficiently metabolized by the green microalga Chlamydomonas reinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were cultivated in batch mode in the light at 17, 31, 44, and 57 mM acetate. Metabolic analyses show that cells grown at 57 mM acetate possess increased contents of all components analyzed (starch, chlorophylls, fatty acids, and proteins), with a three-fold increased volumetric biomass yield compared to cells cultivated at 17 mM acetate at the entry of stationary phase. Physiological analyses highlight the importance of photosynthesis for the low-acetate and exponential-phase samples. The stationary phase is reached when acetate is depleted, except for the cells grown at 57 mM acetate, which still divide until ammonium exhaustion. Surprisal analysis of the transcriptomics data supports the biological significance of our experiments. This allows the establishment of a model for acetate assimilation, its transcriptional regulation and the identification of candidates for genetic engineering of this metabolic pathway. Altogether, our analyses suggest that growing at high-acetate concentrations could increase biomass productivities in low-light and CO(2)-limiting air-bubbled medium for biotechnology. MDPI 2019-10-31 /pmc/articles/PMC6912441/ /pubmed/31683711 http://dx.doi.org/10.3390/cells8111367 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bogaert, Kenny A. Perez, Emilie Rumin, Judith Giltay, Axel Carone, Michele Coosemans, Nadine Radoux, Michele Eppe, Gauthier Levine, Raphael D. Remacle, Francoise Remacle, Claire Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title | Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title_full | Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title_fullStr | Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title_full_unstemmed | Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title_short | Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations |
title_sort | metabolic, physiological, and transcriptomics analysis of batch cultures of the green microalga chlamydomonas grown on different acetate concentrations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912441/ https://www.ncbi.nlm.nih.gov/pubmed/31683711 http://dx.doi.org/10.3390/cells8111367 |
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