Effects of Dietary Apple Polyphenols Supplementation on Hepatic Fat Deposition and Antioxidant Capacity in Finishing Pigs

SIMPLE SUMMARY: Excessive fat deposition (5–10%) in the liver could lead to liver damage and nonalcohol fatty liver disease (NAFLD). However, there is no satisfactory safe and effective measure of preventive and therapeutic treatments so far. Thus, the prevention of excessive fat deposition through diet modification might be a better strategy to protect humans from metabolic diseases. Due to the anatomical and physiological similarities between humans and pigs, the present study took the finishing pig as an animal model to investigate the effects of apple polyphenols on hepatic fat deposition and antioxidant capacity and their mechanisms. The present study indicated that apple polyphenols might be an effective dietary supplementation for decreasing the excessive fat deposition in liver tissue, improving lipid profiles and increasing the antioxidant capacity of finishing pigs. This study provides a better preventive strategy to protect humans from excessive fat deposition in the liver. ABSTRACT: Excessive fat deposition in the liver could lead to fatty liver and an increased risk of many metabolic diseases. Apple polyphenols (APPs), the major antioxidants in apples, possess wide-ranging beneficial biological functions. The present study aimed to investigate the effects of APPs on hepatic fat deposition and antioxidant capacity in finishing pigs, and their mechanisms. Results showed that APPs improved lipid profiles, increased antioxidant enzyme activities and reduced the fat deposition in the liver. In the liver, SOD1, CAT, GPX1, GST, NF-E2-related nuclear factor 2 (Nrf2), hormone sensitive lipase (HSL), carnitine palmitoyl transferase-1b (CPT1b), peroxisome proliferator-activated receptor α (PPARα), cholesterol 7α-hydroxylase (CYP7A1) and low-density lipoprotein receptor (LDL-R) mRNA levels were increased by APPs, while Kelch-like ECH-associated protein 1 (Keap1) mRNA level, C16:0 and C20:4n-6 proportions and Δ9-18 dehydrogenase activity were decreased. In conclusion, this study indicated that APPs might be an effective dietary supplementation for improving lipid profiles, increasing antioxidant capacities and decreasing fat deposition in the liver.