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Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle

SIMPLE SUMMARY: Chinese indigenous cattle were clustered into two groups of southern and northern breeds. The populations distributed in the hot south are mainly dominated by Bos indicus, and the populations distributed in the cold north are mainly dominated by Bos taurus. Myosin-1a (MYO1A) gene is...

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Autores principales: Jia, Peng, Cai, Cuicui, Qu, Kaixing, Chen, Ningbo, Jia, Yutang, Hanif, Quratulain, Liu, Jianyong, Zhang, Jicai, Chen, Hong, Huang, Bizhi, Lei, Chuzhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912737/
https://www.ncbi.nlm.nih.gov/pubmed/31766183
http://dx.doi.org/10.3390/ani9110964
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author Jia, Peng
Cai, Cuicui
Qu, Kaixing
Chen, Ningbo
Jia, Yutang
Hanif, Quratulain
Liu, Jianyong
Zhang, Jicai
Chen, Hong
Huang, Bizhi
Lei, Chuzhao
author_facet Jia, Peng
Cai, Cuicui
Qu, Kaixing
Chen, Ningbo
Jia, Yutang
Hanif, Quratulain
Liu, Jianyong
Zhang, Jicai
Chen, Hong
Huang, Bizhi
Lei, Chuzhao
author_sort Jia, Peng
collection PubMed
description SIMPLE SUMMARY: Chinese indigenous cattle were clustered into two groups of southern and northern breeds. The populations distributed in the hot south are mainly dominated by Bos indicus, and the populations distributed in the cold north are mainly dominated by Bos taurus. Myosin-1a (MYO1A) gene is a candidate gene related to pigmentation, which may be closely related to the heat tolerance traits of cattle. Therefore, our study aimed to explore the relationship between MYO1A gene and heat tolerance in Chinese indigenous cattle. ABSTRACT: Based on the previous GWAS research related to bovine heat tolerance trait, this study aimed to explore the effect of myosin-1a (MYO1A) gene on bovine heat tolerance trait, and find the molecular markers related to the heat tolerance of Chinese cattle. In our study, four novel candidate SNPs highly conserved in B. indicus breeds but barely existed in B. taurus were identified in MYO1A gene according to Bovine Genome Variation Database and Selective Signatures (BGVD). PCR and DNA sequencing were used to genotype 1072 individuals including 34 Chinese indigenous cattle breeds as well as Angus and Indian zebu. Two synonymous mutations (rs208210464 and rs110123931), one missense mutation (rs209999142; Phe172Ser), and one intron mutation (rs135771836) were detected. The frequencies of mutant alleles of the four SNPs gradually increased from northern groups to southern groups of Chinese cattle, which was consistent with the distribution of various climatic conditions of China. Additionally, four SNPs were significantly associated with four climatic conditions including annual mean temperature (T), relative humidity (H), temperature-humidity index (THI), and average annual sunshine hours (100-cloudiness) (SR). Among these, rs209999142 and Hap 1/1 had better performance than others. Our results suggested that rs209999142 was associated with heat-tolerance trait and rs208210464, rs110123931, and rs135771836 showed high phenotypic effect on heat-tolerance trait because of the strong linkage with rs209999142. These SNPs could be used as candidates for marker-assisted selection (MAS) in cattle breeding.
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spelling pubmed-69127372020-01-02 Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle Jia, Peng Cai, Cuicui Qu, Kaixing Chen, Ningbo Jia, Yutang Hanif, Quratulain Liu, Jianyong Zhang, Jicai Chen, Hong Huang, Bizhi Lei, Chuzhao Animals (Basel) Article SIMPLE SUMMARY: Chinese indigenous cattle were clustered into two groups of southern and northern breeds. The populations distributed in the hot south are mainly dominated by Bos indicus, and the populations distributed in the cold north are mainly dominated by Bos taurus. Myosin-1a (MYO1A) gene is a candidate gene related to pigmentation, which may be closely related to the heat tolerance traits of cattle. Therefore, our study aimed to explore the relationship between MYO1A gene and heat tolerance in Chinese indigenous cattle. ABSTRACT: Based on the previous GWAS research related to bovine heat tolerance trait, this study aimed to explore the effect of myosin-1a (MYO1A) gene on bovine heat tolerance trait, and find the molecular markers related to the heat tolerance of Chinese cattle. In our study, four novel candidate SNPs highly conserved in B. indicus breeds but barely existed in B. taurus were identified in MYO1A gene according to Bovine Genome Variation Database and Selective Signatures (BGVD). PCR and DNA sequencing were used to genotype 1072 individuals including 34 Chinese indigenous cattle breeds as well as Angus and Indian zebu. Two synonymous mutations (rs208210464 and rs110123931), one missense mutation (rs209999142; Phe172Ser), and one intron mutation (rs135771836) were detected. The frequencies of mutant alleles of the four SNPs gradually increased from northern groups to southern groups of Chinese cattle, which was consistent with the distribution of various climatic conditions of China. Additionally, four SNPs were significantly associated with four climatic conditions including annual mean temperature (T), relative humidity (H), temperature-humidity index (THI), and average annual sunshine hours (100-cloudiness) (SR). Among these, rs209999142 and Hap 1/1 had better performance than others. Our results suggested that rs209999142 was associated with heat-tolerance trait and rs208210464, rs110123931, and rs135771836 showed high phenotypic effect on heat-tolerance trait because of the strong linkage with rs209999142. These SNPs could be used as candidates for marker-assisted selection (MAS) in cattle breeding. MDPI 2019-11-13 /pmc/articles/PMC6912737/ /pubmed/31766183 http://dx.doi.org/10.3390/ani9110964 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jia, Peng
Cai, Cuicui
Qu, Kaixing
Chen, Ningbo
Jia, Yutang
Hanif, Quratulain
Liu, Jianyong
Zhang, Jicai
Chen, Hong
Huang, Bizhi
Lei, Chuzhao
Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title_full Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title_fullStr Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title_full_unstemmed Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title_short Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle
title_sort four novel snps of myo1a gene associated with heat-tolerance in chinese cattle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912737/
https://www.ncbi.nlm.nih.gov/pubmed/31766183
http://dx.doi.org/10.3390/ani9110964
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