Cargando…
Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives
Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912738/ https://www.ncbi.nlm.nih.gov/pubmed/31717640 http://dx.doi.org/10.3390/jcm8111935 |
_version_ | 1783479527788249088 |
---|---|
author | Sadeghpour Heravi, Fatemah Zakrzewski, Martha Vickery, Karen G. Armstrong, David Hu, Honghua |
author_facet | Sadeghpour Heravi, Fatemah Zakrzewski, Martha Vickery, Karen G. Armstrong, David Hu, Honghua |
author_sort | Sadeghpour Heravi, Fatemah |
collection | PubMed |
description | Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches. |
format | Online Article Text |
id | pubmed-6912738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69127382020-01-02 Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives Sadeghpour Heravi, Fatemah Zakrzewski, Martha Vickery, Karen G. Armstrong, David Hu, Honghua J Clin Med Review Diabetic foot ulcers (DFUs) and diabetic foot infections (DFIs) are associated with reduced patient quality of life, lower-extremity amputation, hospitalization, and high morbidity and mortality. Diverse bacterial communities have been identified in DFUs/DFIs, playing a significant role in infection prognosis. However, due to the high heterogeneity of bacterial communities colonized in DFUs/DFIs, culture-based methods may not isolate all of the bacterial population or unexpected microorganisms. Recently, high sensitivity and specificity of DNA (metagenomics) and RNA (metatranscriptomics) technologies have addressed limitations of culture-based methods and have taken a step beyond bacterial identification. As a consequence, new advances obtained from DNA- and RNA-based techniques for bacterial identification can improve therapeutic approaches. This review evaluated the current state of play in aetiology of DFUs/DFIs on culture and molecular approaches, and discussed the impact of metagenomic and metatranscriptomic methods in bacterial identification approaches. MDPI 2019-11-10 /pmc/articles/PMC6912738/ /pubmed/31717640 http://dx.doi.org/10.3390/jcm8111935 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Sadeghpour Heravi, Fatemah Zakrzewski, Martha Vickery, Karen G. Armstrong, David Hu, Honghua Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title | Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title_full | Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title_fullStr | Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title_full_unstemmed | Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title_short | Bacterial Diversity of Diabetic Foot Ulcers: Current Status and Future Prospectives |
title_sort | bacterial diversity of diabetic foot ulcers: current status and future prospectives |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912738/ https://www.ncbi.nlm.nih.gov/pubmed/31717640 http://dx.doi.org/10.3390/jcm8111935 |
work_keys_str_mv | AT sadeghpourheravifatemah bacterialdiversityofdiabeticfootulcerscurrentstatusandfutureprospectives AT zakrzewskimartha bacterialdiversityofdiabeticfootulcerscurrentstatusandfutureprospectives AT vickerykaren bacterialdiversityofdiabeticfootulcerscurrentstatusandfutureprospectives AT garmstrongdavid bacterialdiversityofdiabeticfootulcerscurrentstatusandfutureprospectives AT huhonghua bacterialdiversityofdiabeticfootulcerscurrentstatusandfutureprospectives |