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Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma

The lethal-7 (let-7) family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i and to assess their value as prognostic markers in uterine leiomyo...

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Autores principales: de Almeida, Bruna Cristine, dos Anjos, Laura Gonzalez, Uno, Miyuki, da Cunha, Isabela Werneck, Soares, Fernando Augusto, Baiocchi, Glauco, Baracat, Edmund Chada, Carvalho, Katia Candido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912804/
https://www.ncbi.nlm.nih.gov/pubmed/31744257
http://dx.doi.org/10.3390/cells8111452
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author de Almeida, Bruna Cristine
dos Anjos, Laura Gonzalez
Uno, Miyuki
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Baiocchi, Glauco
Baracat, Edmund Chada
Carvalho, Katia Candido
author_facet de Almeida, Bruna Cristine
dos Anjos, Laura Gonzalez
Uno, Miyuki
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Baiocchi, Glauco
Baracat, Edmund Chada
Carvalho, Katia Candido
author_sort de Almeida, Bruna Cristine
collection PubMed
description The lethal-7 (let-7) family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i and to assess their value as prognostic markers in uterine leiomyosarcoma (LMS) patients. The miRNAs expression profile was assessed in 34 LMS and 13 normal myometrium (MM) paraffin-embedded samples. All let-7 family members showed downregulation in LMS. Our findings showed that patients with let-7e downregulation had worse overall survival (OS) and is an independent prognostic factor (hazard ratio [HR] = 2.24). In addition, almost half the patients had distant metastasis. LMS patients with downregulated let-7b and let-7d had worse disease-free survival (DFS); they are not independent prognostic factors (HR = 2.65). Patients’ ages were associated with let-7d, let-7e and let-7f (p = 0.0160) downregulation. In conclusion, all the let-7 family members were downregulated in LMS patients, and the greater the loss of expression of these molecules, the greater their relationship with worse prognosis of patients. Let-7e expression might influence the OS, while let-7b and le-7d might influence the DFS. The lowest expression levels of let-7d, let-7e, and let-7f were associated with the oldest patients. Our findings indicate strong evidence of let-7’s role as a potential prognostic biomarker in LMS.
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spelling pubmed-69128042020-01-02 Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma de Almeida, Bruna Cristine dos Anjos, Laura Gonzalez Uno, Miyuki da Cunha, Isabela Werneck Soares, Fernando Augusto Baiocchi, Glauco Baracat, Edmund Chada Carvalho, Katia Candido Cells Article The lethal-7 (let-7) family is an important microRNA (miRNA) group that usually exerts functions as a tumor suppressor. We aimed to evaluate the expression profile of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i and to assess their value as prognostic markers in uterine leiomyosarcoma (LMS) patients. The miRNAs expression profile was assessed in 34 LMS and 13 normal myometrium (MM) paraffin-embedded samples. All let-7 family members showed downregulation in LMS. Our findings showed that patients with let-7e downregulation had worse overall survival (OS) and is an independent prognostic factor (hazard ratio [HR] = 2.24). In addition, almost half the patients had distant metastasis. LMS patients with downregulated let-7b and let-7d had worse disease-free survival (DFS); they are not independent prognostic factors (HR = 2.65). Patients’ ages were associated with let-7d, let-7e and let-7f (p = 0.0160) downregulation. In conclusion, all the let-7 family members were downregulated in LMS patients, and the greater the loss of expression of these molecules, the greater their relationship with worse prognosis of patients. Let-7e expression might influence the OS, while let-7b and le-7d might influence the DFS. The lowest expression levels of let-7d, let-7e, and let-7f were associated with the oldest patients. Our findings indicate strong evidence of let-7’s role as a potential prognostic biomarker in LMS. MDPI 2019-11-17 /pmc/articles/PMC6912804/ /pubmed/31744257 http://dx.doi.org/10.3390/cells8111452 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
de Almeida, Bruna Cristine
dos Anjos, Laura Gonzalez
Uno, Miyuki
da Cunha, Isabela Werneck
Soares, Fernando Augusto
Baiocchi, Glauco
Baracat, Edmund Chada
Carvalho, Katia Candido
Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title_full Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title_fullStr Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title_full_unstemmed Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title_short Let-7 miRNA’s Expression Profile and Its Potential Prognostic Role in Uterine Leiomyosarcoma
title_sort let-7 mirna’s expression profile and its potential prognostic role in uterine leiomyosarcoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912804/
https://www.ncbi.nlm.nih.gov/pubmed/31744257
http://dx.doi.org/10.3390/cells8111452
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