The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()

PURPOSE: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. METHODS: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confi...

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Autores principales: Trevisan, Alexandre Maximiliano, Cogliati, Bruno, Homem, Adriana Ribeiro, Aloiav, Thiago Pinheiro Arrais, de Aquino, Nelson, Moreira, Jairo Marques, Reno, Leonardo da Cruz, Naumann, Alexandre Moulin, Galvão, Flavio Henrique Ferreira, Andraus, Wellington, D'Albuquerque, Luiz Augusto Carneiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912844/
https://www.ncbi.nlm.nih.gov/pubmed/31851211
http://dx.doi.org/10.1590/s0102-865020190100000003
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author Trevisan, Alexandre Maximiliano
Cogliati, Bruno
Homem, Adriana Ribeiro
Aloiav, Thiago Pinheiro Arrais
de Aquino, Nelson
Moreira, Jairo Marques
Reno, Leonardo da Cruz
Naumann, Alexandre Moulin
Galvão, Flavio Henrique Ferreira
Andraus, Wellington
D'Albuquerque, Luiz Augusto Carneiro
author_facet Trevisan, Alexandre Maximiliano
Cogliati, Bruno
Homem, Adriana Ribeiro
Aloiav, Thiago Pinheiro Arrais
de Aquino, Nelson
Moreira, Jairo Marques
Reno, Leonardo da Cruz
Naumann, Alexandre Moulin
Galvão, Flavio Henrique Ferreira
Andraus, Wellington
D'Albuquerque, Luiz Augusto Carneiro
author_sort Trevisan, Alexandre Maximiliano
collection PubMed
description PURPOSE: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. METHODS: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. RESULTS: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. CONCLUSIONS: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.
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spelling pubmed-69128442019-12-18 The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43() Trevisan, Alexandre Maximiliano Cogliati, Bruno Homem, Adriana Ribeiro Aloiav, Thiago Pinheiro Arrais de Aquino, Nelson Moreira, Jairo Marques Reno, Leonardo da Cruz Naumann, Alexandre Moulin Galvão, Flavio Henrique Ferreira Andraus, Wellington D'Albuquerque, Luiz Augusto Carneiro Acta Cir Bras Original Article PURPOSE: To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury. METHODS: We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology. RESULTS: The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group. CONCLUSIONS: This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2019-12-13 /pmc/articles/PMC6912844/ /pubmed/31851211 http://dx.doi.org/10.1590/s0102-865020190100000003 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Trevisan, Alexandre Maximiliano
Cogliati, Bruno
Homem, Adriana Ribeiro
Aloiav, Thiago Pinheiro Arrais
de Aquino, Nelson
Moreira, Jairo Marques
Reno, Leonardo da Cruz
Naumann, Alexandre Moulin
Galvão, Flavio Henrique Ferreira
Andraus, Wellington
D'Albuquerque, Luiz Augusto Carneiro
The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title_full The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title_fullStr The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title_full_unstemmed The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title_short The liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
title_sort liver injury following ischemia and reperfusion is worse in experimental knockout heterozygote mouse model for expression of connexin 43()
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6912844/
https://www.ncbi.nlm.nih.gov/pubmed/31851211
http://dx.doi.org/10.1590/s0102-865020190100000003
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