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Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors

BACKGROUND: Furfural and acetic acid are the two major inhibitors generated during lignocellulose pretreatment and hydrolysis, would severely inhibit the cell growth, metabolism, and ethanol fermentation efficiency of Zymomonas mobilis. Effective genome shuffling mediated by protoplast electrofusion...

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Autores principales: Wang, Weiting, Wu, Bo, Qin, Han, Liu, Panting, Qin, Yao, Duan, Guowei, Hu, Guoquan, He, Mingxiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913010/
https://www.ncbi.nlm.nih.gov/pubmed/31890016
http://dx.doi.org/10.1186/s13068-019-1631-4
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author Wang, Weiting
Wu, Bo
Qin, Han
Liu, Panting
Qin, Yao
Duan, Guowei
Hu, Guoquan
He, Mingxiong
author_facet Wang, Weiting
Wu, Bo
Qin, Han
Liu, Panting
Qin, Yao
Duan, Guowei
Hu, Guoquan
He, Mingxiong
author_sort Wang, Weiting
collection PubMed
description BACKGROUND: Furfural and acetic acid are the two major inhibitors generated during lignocellulose pretreatment and hydrolysis, would severely inhibit the cell growth, metabolism, and ethanol fermentation efficiency of Zymomonas mobilis. Effective genome shuffling mediated by protoplast electrofusion was developed and then applied to Z. mobilis. RESULTS: After two rounds of genome shuffling, 10 different mutants with improved cell growth and ethanol yield in the presence of 5.0 g/L acetic acid and 3.0 g/L furfural were obtained. The two most prominent genome-shuffled strains, 532 and 533, were further investigated along with parental strains in the presence of 7.0 g/L acetic acid and 3.0 g/L furfural. The results showed that mutants 532 and 533 were superior to the parental strain AQ8-1 in the presence of 7.0 g/L acetic acid, with a shorter fermentation time (30 h) and higher productivity than AQ8-1. Mutant 533 exhibited subtle differences from parental strain F34 in the presence of 3.0 g/L furfural. Mutations present in 10 genome-shuffled strains were identified via whole-genome resequencing, and the source of each mutation was identified as either de novo mutation or recombination of the parent genes. CONCLUSIONS: These results indicate that genome shuffling is an efficient method for enhancing stress tolerance in Z. mobilis. The engineered strains generated in this study could be potential cellulosic ethanol producers in the future.
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spelling pubmed-69130102019-12-30 Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors Wang, Weiting Wu, Bo Qin, Han Liu, Panting Qin, Yao Duan, Guowei Hu, Guoquan He, Mingxiong Biotechnol Biofuels Research BACKGROUND: Furfural and acetic acid are the two major inhibitors generated during lignocellulose pretreatment and hydrolysis, would severely inhibit the cell growth, metabolism, and ethanol fermentation efficiency of Zymomonas mobilis. Effective genome shuffling mediated by protoplast electrofusion was developed and then applied to Z. mobilis. RESULTS: After two rounds of genome shuffling, 10 different mutants with improved cell growth and ethanol yield in the presence of 5.0 g/L acetic acid and 3.0 g/L furfural were obtained. The two most prominent genome-shuffled strains, 532 and 533, were further investigated along with parental strains in the presence of 7.0 g/L acetic acid and 3.0 g/L furfural. The results showed that mutants 532 and 533 were superior to the parental strain AQ8-1 in the presence of 7.0 g/L acetic acid, with a shorter fermentation time (30 h) and higher productivity than AQ8-1. Mutant 533 exhibited subtle differences from parental strain F34 in the presence of 3.0 g/L furfural. Mutations present in 10 genome-shuffled strains were identified via whole-genome resequencing, and the source of each mutation was identified as either de novo mutation or recombination of the parent genes. CONCLUSIONS: These results indicate that genome shuffling is an efficient method for enhancing stress tolerance in Z. mobilis. The engineered strains generated in this study could be potential cellulosic ethanol producers in the future. BioMed Central 2019-12-16 /pmc/articles/PMC6913010/ /pubmed/31890016 http://dx.doi.org/10.1186/s13068-019-1631-4 Text en © The Author(s) 2019 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Weiting
Wu, Bo
Qin, Han
Liu, Panting
Qin, Yao
Duan, Guowei
Hu, Guoquan
He, Mingxiong
Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title_full Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title_fullStr Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title_full_unstemmed Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title_short Genome shuffling enhances stress tolerance of Zymomonas mobilis to two inhibitors
title_sort genome shuffling enhances stress tolerance of zymomonas mobilis to two inhibitors
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913010/
https://www.ncbi.nlm.nih.gov/pubmed/31890016
http://dx.doi.org/10.1186/s13068-019-1631-4
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