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Live-cell imaging of single mRNA dynamics using split superfolder green fluorescent proteins with minimal background
The MS2 system, with an MS2 binding site (MBS) and an MS2 coat protein fused to a fluorescent protein (MCP–FP), has been widely used to fluorescently label mRNA in live cells. However, one of its limitations is the constant background fluorescence signal generated from free MCP–FPs. To overcome this...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Cold Spring Harbor Laboratory Press
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913125/ https://www.ncbi.nlm.nih.gov/pubmed/31641028 http://dx.doi.org/10.1261/rna.067835.118 |