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Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages

Obesity is a metabolic condition associated with multiple health problems such as endocrine and metabolic dysfunction and chronic inflammation in adipose tissues. In this study, the ADSCs could be stimulated to differentiate into brown adipocyte with rosiglitazone treatment based on the Oil-Red-O st...

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Autores principales: Zhang, Wen-Chao, Qin, Feng, Wang, Xiao-Jun, Liu, Zhi-Fei, Zhu, Lin, Zeng, Ang, Zhang, Ming-Zi, Yu, Nan-Ze, Long, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913309/
https://www.ncbi.nlm.nih.gov/pubmed/31871424
http://dx.doi.org/10.1155/2019/1731540
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author Zhang, Wen-Chao
Qin, Feng
Wang, Xiao-Jun
Liu, Zhi-Fei
Zhu, Lin
Zeng, Ang
Zhang, Ming-Zi
Yu, Nan-Ze
Long, Xiao
author_facet Zhang, Wen-Chao
Qin, Feng
Wang, Xiao-Jun
Liu, Zhi-Fei
Zhu, Lin
Zeng, Ang
Zhang, Ming-Zi
Yu, Nan-Ze
Long, Xiao
author_sort Zhang, Wen-Chao
collection PubMed
description Obesity is a metabolic condition associated with multiple health problems such as endocrine and metabolic dysfunction and chronic inflammation in adipose tissues. In this study, the ADSCs could be stimulated to differentiate into brown adipocyte with rosiglitazone treatment based on the Oil-Red-O staining trial. Furthermore, the multilocular lipid droplets located in the center was increased in differentiated brown adipocytes, and brown fat-associated proteins, UCP1, PPAR-γ, and LPL were highly expressed in brown adipocytes differentiated from ADSCs. Additionally, the results of animal experiments showed that both weight and amount of VLDL and LDL were decreased in the serum of obese mice after transplantation of rosiglitazone-induced brown adipocytes, while the level of HDL increased. Moreover, the proteins associated with lipid metabolism, LPA and UCP1, were downregulated, and the inflammatory response was suppressed through inhibition of the ITGAM/NF-κB-mediated proinflammatory responses and polarization of M2 macrophages. Similarly, the amounts of proinflammatory cytokines, TNF-α, IL-6, and IL-1β were decreased after rosiglitazone-induced brown adipocyte transplantation. On the contrary, anti-inflammatory cytokine IL-10 was significantly increased in both groups of obese mice, with or without brown adipocyte transplantation. Therefore, the adipose-derived stromal cells with induced browning could promote lipid consumption and alternative polarization of M2 macrophages to attenuate adipose inflammation in obesity mouse models, which thus provides a potential therapy for obesity.
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spelling pubmed-69133092019-12-23 Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages Zhang, Wen-Chao Qin, Feng Wang, Xiao-Jun Liu, Zhi-Fei Zhu, Lin Zeng, Ang Zhang, Ming-Zi Yu, Nan-Ze Long, Xiao Mediators Inflamm Research Article Obesity is a metabolic condition associated with multiple health problems such as endocrine and metabolic dysfunction and chronic inflammation in adipose tissues. In this study, the ADSCs could be stimulated to differentiate into brown adipocyte with rosiglitazone treatment based on the Oil-Red-O staining trial. Furthermore, the multilocular lipid droplets located in the center was increased in differentiated brown adipocytes, and brown fat-associated proteins, UCP1, PPAR-γ, and LPL were highly expressed in brown adipocytes differentiated from ADSCs. Additionally, the results of animal experiments showed that both weight and amount of VLDL and LDL were decreased in the serum of obese mice after transplantation of rosiglitazone-induced brown adipocytes, while the level of HDL increased. Moreover, the proteins associated with lipid metabolism, LPA and UCP1, were downregulated, and the inflammatory response was suppressed through inhibition of the ITGAM/NF-κB-mediated proinflammatory responses and polarization of M2 macrophages. Similarly, the amounts of proinflammatory cytokines, TNF-α, IL-6, and IL-1β were decreased after rosiglitazone-induced brown adipocyte transplantation. On the contrary, anti-inflammatory cytokine IL-10 was significantly increased in both groups of obese mice, with or without brown adipocyte transplantation. Therefore, the adipose-derived stromal cells with induced browning could promote lipid consumption and alternative polarization of M2 macrophages to attenuate adipose inflammation in obesity mouse models, which thus provides a potential therapy for obesity. Hindawi 2019-12-04 /pmc/articles/PMC6913309/ /pubmed/31871424 http://dx.doi.org/10.1155/2019/1731540 Text en Copyright © 2019 Wen-Chao Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Wen-Chao
Qin, Feng
Wang, Xiao-Jun
Liu, Zhi-Fei
Zhu, Lin
Zeng, Ang
Zhang, Ming-Zi
Yu, Nan-Ze
Long, Xiao
Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title_full Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title_fullStr Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title_full_unstemmed Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title_short Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
title_sort adipose-derived stromal cells attenuate adipose inflammation in obesity through adipocyte browning and polarization of m2 macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913309/
https://www.ncbi.nlm.nih.gov/pubmed/31871424
http://dx.doi.org/10.1155/2019/1731540
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