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Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans

Phosphorylation is one of the critical protein modifications, which can lead to changing the activity of the proteins and regulating a variety of biological processes. Therefore, it is essential to properly maintain the phosphorylation level on proteins by balancing the activity of kinases and phosp...

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Autores principales: Kim, Seung Hyun, Jung, Hana, Ahnn, Joohong, Lee, Sun-Kyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913648/
https://www.ncbi.nlm.nih.gov/pubmed/31853377
http://dx.doi.org/10.1080/19768354.2019.1687584
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author Kim, Seung Hyun
Jung, Hana
Ahnn, Joohong
Lee, Sun-Kyung
author_facet Kim, Seung Hyun
Jung, Hana
Ahnn, Joohong
Lee, Sun-Kyung
author_sort Kim, Seung Hyun
collection PubMed
description Phosphorylation is one of the critical protein modifications, which can lead to changing the activity of the proteins and regulating a variety of biological processes. Therefore, it is essential to properly maintain the phosphorylation level on proteins by balancing the activity of kinases and phosphatases. In this study, we report that calcineurin, a serine/threonine phosphatase, counteracts with a salt inducible kinase (SIK) to control male tail development in Caenorhabditis elegans. The counteracting regulation is cell lineage-dependent; the number of defective rays from T lineage in animals lacking calcineurin tax-6 is decreased by knock-down of SIK kin-29. This result is in contrast with the knock-down of bone marrow protein (BMP) receptor kinase sma-6, which slightly aggravates the T lineage defect. Also, sma-6 knock-down results in modest defect in ray 1 of V5 lineage in the absence of tax-6 activity. Finally, knock-down of a tyrosine phosphatase cdc-25.3 does not affect the defective ray phenotype of calcineurin tax-6 loss-of-function(lf) mutants. Altogether, these results suggest that balanced phosphorylation mediated by tax-6 and kin-29 is required for proper development of T lineage rays, and tax-6 and sma-6 may function in a parallel pathway in the developmental process of V5 lineage ray 1. This study emphasizes the elaborated developmental process of male ray formation, in which carefully coordinated expression of various genes is essential.
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spelling pubmed-69136482019-12-18 Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans Kim, Seung Hyun Jung, Hana Ahnn, Joohong Lee, Sun-Kyung Anim Cells Syst (Seoul) Developmental Biology Phosphorylation is one of the critical protein modifications, which can lead to changing the activity of the proteins and regulating a variety of biological processes. Therefore, it is essential to properly maintain the phosphorylation level on proteins by balancing the activity of kinases and phosphatases. In this study, we report that calcineurin, a serine/threonine phosphatase, counteracts with a salt inducible kinase (SIK) to control male tail development in Caenorhabditis elegans. The counteracting regulation is cell lineage-dependent; the number of defective rays from T lineage in animals lacking calcineurin tax-6 is decreased by knock-down of SIK kin-29. This result is in contrast with the knock-down of bone marrow protein (BMP) receptor kinase sma-6, which slightly aggravates the T lineage defect. Also, sma-6 knock-down results in modest defect in ray 1 of V5 lineage in the absence of tax-6 activity. Finally, knock-down of a tyrosine phosphatase cdc-25.3 does not affect the defective ray phenotype of calcineurin tax-6 loss-of-function(lf) mutants. Altogether, these results suggest that balanced phosphorylation mediated by tax-6 and kin-29 is required for proper development of T lineage rays, and tax-6 and sma-6 may function in a parallel pathway in the developmental process of V5 lineage ray 1. This study emphasizes the elaborated developmental process of male ray formation, in which carefully coordinated expression of various genes is essential. Taylor & Francis 2019-11-16 /pmc/articles/PMC6913648/ /pubmed/31853377 http://dx.doi.org/10.1080/19768354.2019.1687584 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Developmental Biology
Kim, Seung Hyun
Jung, Hana
Ahnn, Joohong
Lee, Sun-Kyung
Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title_full Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title_fullStr Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title_full_unstemmed Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title_short Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
title_sort calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in caenorhabditis elegans
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913648/
https://www.ncbi.nlm.nih.gov/pubmed/31853377
http://dx.doi.org/10.1080/19768354.2019.1687584
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