Cargando…
Phase relationships in two-dimensional mass spectrometry
Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to t...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914722/ https://www.ncbi.nlm.nih.gov/pubmed/31617086 http://dx.doi.org/10.1007/s13361-019-02308-1 |
_version_ | 1783479867550990336 |
---|---|
author | van Agthoven, Maria A. Kilgour, David P. A. Lynch, Alice M. Barrow, Mark P. Morgan, Tomos E. Wootton, Christopher A. Chiron, Lionel Delsuc, Marc-André O’Connor, Peter B. |
author_facet | van Agthoven, Maria A. Kilgour, David P. A. Lynch, Alice M. Barrow, Mark P. Morgan, Tomos E. Wootton, Christopher A. Chiron, Lionel Delsuc, Marc-André O’Connor, Peter B. |
author_sort | van Agthoven, Maria A. |
collection | PubMed |
description | Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to the calculation of phase-corrected absorption-mode 2D mass spectra. 2D MS datasets have a phase that is defined differently in each dimension. In both dimensions, the phase behavior of precursor and fragment ions is found to be different. The dependence of the phase for both precursor and fragment ion signals on various parameters (e.g., modulation frequency, shape of the fragmentation zone) is discussed. Experimental data confirms the theoretical calculations of the phase in each dimension. Understanding the phase relationships in a 2D mass spectrum is beneficial to the development of possible algorithms for phase correction, which may improve both the signal-to-noise ratio and the resolving power of peaks in 2D mass spectra. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-019-02308-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6914722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-69147222020-01-02 Phase relationships in two-dimensional mass spectrometry van Agthoven, Maria A. Kilgour, David P. A. Lynch, Alice M. Barrow, Mark P. Morgan, Tomos E. Wootton, Christopher A. Chiron, Lionel Delsuc, Marc-André O’Connor, Peter B. J Am Soc Mass Spectrom Research Article Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to the calculation of phase-corrected absorption-mode 2D mass spectra. 2D MS datasets have a phase that is defined differently in each dimension. In both dimensions, the phase behavior of precursor and fragment ions is found to be different. The dependence of the phase for both precursor and fragment ion signals on various parameters (e.g., modulation frequency, shape of the fragmentation zone) is discussed. Experimental data confirms the theoretical calculations of the phase in each dimension. Understanding the phase relationships in a 2D mass spectrum is beneficial to the development of possible algorithms for phase correction, which may improve both the signal-to-noise ratio and the resolving power of peaks in 2D mass spectra. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-019-02308-1) contains supplementary material, which is available to authorized users. Springer US 2019-10-15 2019 /pmc/articles/PMC6914722/ /pubmed/31617086 http://dx.doi.org/10.1007/s13361-019-02308-1 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Article van Agthoven, Maria A. Kilgour, David P. A. Lynch, Alice M. Barrow, Mark P. Morgan, Tomos E. Wootton, Christopher A. Chiron, Lionel Delsuc, Marc-André O’Connor, Peter B. Phase relationships in two-dimensional mass spectrometry |
title | Phase relationships in two-dimensional mass spectrometry |
title_full | Phase relationships in two-dimensional mass spectrometry |
title_fullStr | Phase relationships in two-dimensional mass spectrometry |
title_full_unstemmed | Phase relationships in two-dimensional mass spectrometry |
title_short | Phase relationships in two-dimensional mass spectrometry |
title_sort | phase relationships in two-dimensional mass spectrometry |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914722/ https://www.ncbi.nlm.nih.gov/pubmed/31617086 http://dx.doi.org/10.1007/s13361-019-02308-1 |
work_keys_str_mv | AT vanagthovenmariaa phaserelationshipsintwodimensionalmassspectrometry AT kilgourdavidpa phaserelationshipsintwodimensionalmassspectrometry AT lynchalicem phaserelationshipsintwodimensionalmassspectrometry AT barrowmarkp phaserelationshipsintwodimensionalmassspectrometry AT morgantomose phaserelationshipsintwodimensionalmassspectrometry AT woottonchristophera phaserelationshipsintwodimensionalmassspectrometry AT chironlionel phaserelationshipsintwodimensionalmassspectrometry AT delsucmarcandre phaserelationshipsintwodimensionalmassspectrometry AT oconnorpeterb phaserelationshipsintwodimensionalmassspectrometry |