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Phase relationships in two-dimensional mass spectrometry

Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to t...

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Autores principales: van Agthoven, Maria A., Kilgour, David P. A., Lynch, Alice M., Barrow, Mark P., Morgan, Tomos E., Wootton, Christopher A., Chiron, Lionel, Delsuc, Marc-André, O’Connor, Peter B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914722/
https://www.ncbi.nlm.nih.gov/pubmed/31617086
http://dx.doi.org/10.1007/s13361-019-02308-1
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author van Agthoven, Maria A.
Kilgour, David P. A.
Lynch, Alice M.
Barrow, Mark P.
Morgan, Tomos E.
Wootton, Christopher A.
Chiron, Lionel
Delsuc, Marc-André
O’Connor, Peter B.
author_facet van Agthoven, Maria A.
Kilgour, David P. A.
Lynch, Alice M.
Barrow, Mark P.
Morgan, Tomos E.
Wootton, Christopher A.
Chiron, Lionel
Delsuc, Marc-André
O’Connor, Peter B.
author_sort van Agthoven, Maria A.
collection PubMed
description Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to the calculation of phase-corrected absorption-mode 2D mass spectra. 2D MS datasets have a phase that is defined differently in each dimension. In both dimensions, the phase behavior of precursor and fragment ions is found to be different. The dependence of the phase for both precursor and fragment ion signals on various parameters (e.g., modulation frequency, shape of the fragmentation zone) is discussed. Experimental data confirms the theoretical calculations of the phase in each dimension. Understanding the phase relationships in a 2D mass spectrum is beneficial to the development of possible algorithms for phase correction, which may improve both the signal-to-noise ratio and the resolving power of peaks in 2D mass spectra. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-019-02308-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-69147222020-01-02 Phase relationships in two-dimensional mass spectrometry van Agthoven, Maria A. Kilgour, David P. A. Lynch, Alice M. Barrow, Mark P. Morgan, Tomos E. Wootton, Christopher A. Chiron, Lionel Delsuc, Marc-André O’Connor, Peter B. J Am Soc Mass Spectrom Research Article Two-dimensional mass spectrometry (2D MS) is a data-independent tandem mass spectrometry technique in which precursor and fragment ion species can be correlated without the need for prior ion isolation. The behavior of phase in 2D Fourier transform mass spectrometry is investigated with respect to the calculation of phase-corrected absorption-mode 2D mass spectra. 2D MS datasets have a phase that is defined differently in each dimension. In both dimensions, the phase behavior of precursor and fragment ions is found to be different. The dependence of the phase for both precursor and fragment ion signals on various parameters (e.g., modulation frequency, shape of the fragmentation zone) is discussed. Experimental data confirms the theoretical calculations of the phase in each dimension. Understanding the phase relationships in a 2D mass spectrum is beneficial to the development of possible algorithms for phase correction, which may improve both the signal-to-noise ratio and the resolving power of peaks in 2D mass spectra. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-019-02308-1) contains supplementary material, which is available to authorized users. Springer US 2019-10-15 2019 /pmc/articles/PMC6914722/ /pubmed/31617086 http://dx.doi.org/10.1007/s13361-019-02308-1 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research Article
van Agthoven, Maria A.
Kilgour, David P. A.
Lynch, Alice M.
Barrow, Mark P.
Morgan, Tomos E.
Wootton, Christopher A.
Chiron, Lionel
Delsuc, Marc-André
O’Connor, Peter B.
Phase relationships in two-dimensional mass spectrometry
title Phase relationships in two-dimensional mass spectrometry
title_full Phase relationships in two-dimensional mass spectrometry
title_fullStr Phase relationships in two-dimensional mass spectrometry
title_full_unstemmed Phase relationships in two-dimensional mass spectrometry
title_short Phase relationships in two-dimensional mass spectrometry
title_sort phase relationships in two-dimensional mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6914722/
https://www.ncbi.nlm.nih.gov/pubmed/31617086
http://dx.doi.org/10.1007/s13361-019-02308-1
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