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Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips

Microfluidic biochip techniques are prominently replacing conventional biochemical analyzers by the integration of all functions necessary for biochemical analysis using microfluidics. The microfluidics of droplets offer exquisite control over the size of microliter samples to satisfy the requiremen...

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Detalles Bibliográficos
Autores principales: Chiu, Yi-Lung, Yadav, Ruchi Ashok Kumar, Huang, Hong-Yuan, Wang, Yi-Wen, Yao, Da-Jeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915428/
https://www.ncbi.nlm.nih.gov/pubmed/31698735
http://dx.doi.org/10.3390/mi10110756
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author Chiu, Yi-Lung
Yadav, Ruchi Ashok Kumar
Huang, Hong-Yuan
Wang, Yi-Wen
Yao, Da-Jeng
author_facet Chiu, Yi-Lung
Yadav, Ruchi Ashok Kumar
Huang, Hong-Yuan
Wang, Yi-Wen
Yao, Da-Jeng
author_sort Chiu, Yi-Lung
collection PubMed
description Microfluidic biochip techniques are prominently replacing conventional biochemical analyzers by the integration of all functions necessary for biochemical analysis using microfluidics. The microfluidics of droplets offer exquisite control over the size of microliter samples to satisfy the requirements of embryo culture, which might involve a size ranging from picoliter to nanoliter. Polydimethylsiloxane (PDMS) is the mainstream material for the fabrication of microfluidic devices due to its excellent biocompatibility and simplicity of fabrication. Herein, we developed a microfluidic biomedical chip on a PDMS substrate that integrated four key functions—generation of a droplet of an emulsion, sorting, expansion and restoration, which were employed in a mouse embryo system to assess reproductive medicine. The main channel of the designed chip had width of 1200 μm and height of 500 μm. The designed microfluidic chips possessed six sections—cleaved into three inlets and three outlets—to study the key functions with five-day embryo culture. The control part of the experiment was conducted with polystyrene (PS) beads (100 μm), the same size as the murine embryos, for the purpose of testing. The outcomes of our work illustrate that the rate of success of the static droplet culture group (87.5%) is only slightly less than that of a conventional group (95%). It clearly demonstrates that a droplet-based microfluidic system can produce a droplet in a volume range from picoliter to nanoliter.
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spelling pubmed-69154282019-12-24 Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips Chiu, Yi-Lung Yadav, Ruchi Ashok Kumar Huang, Hong-Yuan Wang, Yi-Wen Yao, Da-Jeng Micromachines (Basel) Article Microfluidic biochip techniques are prominently replacing conventional biochemical analyzers by the integration of all functions necessary for biochemical analysis using microfluidics. The microfluidics of droplets offer exquisite control over the size of microliter samples to satisfy the requirements of embryo culture, which might involve a size ranging from picoliter to nanoliter. Polydimethylsiloxane (PDMS) is the mainstream material for the fabrication of microfluidic devices due to its excellent biocompatibility and simplicity of fabrication. Herein, we developed a microfluidic biomedical chip on a PDMS substrate that integrated four key functions—generation of a droplet of an emulsion, sorting, expansion and restoration, which were employed in a mouse embryo system to assess reproductive medicine. The main channel of the designed chip had width of 1200 μm and height of 500 μm. The designed microfluidic chips possessed six sections—cleaved into three inlets and three outlets—to study the key functions with five-day embryo culture. The control part of the experiment was conducted with polystyrene (PS) beads (100 μm), the same size as the murine embryos, for the purpose of testing. The outcomes of our work illustrate that the rate of success of the static droplet culture group (87.5%) is only slightly less than that of a conventional group (95%). It clearly demonstrates that a droplet-based microfluidic system can produce a droplet in a volume range from picoliter to nanoliter. MDPI 2019-11-06 /pmc/articles/PMC6915428/ /pubmed/31698735 http://dx.doi.org/10.3390/mi10110756 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chiu, Yi-Lung
Yadav, Ruchi Ashok Kumar
Huang, Hong-Yuan
Wang, Yi-Wen
Yao, Da-Jeng
Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title_full Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title_fullStr Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title_full_unstemmed Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title_short Unveiling the Potential of Droplet Generation, Sorting, Expansion, and Restoration in Microfluidic Biochips
title_sort unveiling the potential of droplet generation, sorting, expansion, and restoration in microfluidic biochips
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915428/
https://www.ncbi.nlm.nih.gov/pubmed/31698735
http://dx.doi.org/10.3390/mi10110756
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