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Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells

Resveratol (RES) and its natural precursor polydatin (PD) are polyphenols that may display a broad variety of beneficial effects including anti-inflammatory properties. This study aimed to investigate the role of RES and PD in the inflammatory process induced by monosodium urate (MSU) and calcium py...

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Autores principales: Oliviero, Francesca, Zamudio-Cuevas, Yessica, Belluzzi, Elisa, Andretto, Lisa, Scanu, Anna, Favero, Marta, Ramonda, Roberta, Ravagnan, Giampietro, López-Reyes, Alberto, Spinella, Paolo, Punzi, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915461/
https://www.ncbi.nlm.nih.gov/pubmed/31703439
http://dx.doi.org/10.3390/foods8110560
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author Oliviero, Francesca
Zamudio-Cuevas, Yessica
Belluzzi, Elisa
Andretto, Lisa
Scanu, Anna
Favero, Marta
Ramonda, Roberta
Ravagnan, Giampietro
López-Reyes, Alberto
Spinella, Paolo
Punzi, Leonardo
author_facet Oliviero, Francesca
Zamudio-Cuevas, Yessica
Belluzzi, Elisa
Andretto, Lisa
Scanu, Anna
Favero, Marta
Ramonda, Roberta
Ravagnan, Giampietro
López-Reyes, Alberto
Spinella, Paolo
Punzi, Leonardo
author_sort Oliviero, Francesca
collection PubMed
description Resveratol (RES) and its natural precursor polydatin (PD) are polyphenols that may display a broad variety of beneficial effects including anti-inflammatory properties. This study aimed to investigate the role of RES and PD in the inflammatory process induced by monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals in vitro. A monocytic cell line (THP-1) was primed for 3 hours with phorbol myristate acetate (100 ng/mL) and stimulated with synthetic MSU (0.05 mg/mL) and CPP (0.025 mg/mL) crystals. RES and PD were added to cultures concurrently with the crystals, or as 2-hour pretreatment. The effect of the two polyphenols was evaluated on intracellular and extracellular IL-1β levels, NACHT-LRRPYD-containing protein-3 (NLRP3) inflammasome expression, reactive oxygen species (ROS) and nitric oxide (NO) production, and the assessment of crystal phagocytosis. RES and PD strongly inhibited IL-1β induced by crystals after cell pretreatment. Cell pretreatment was effective also in reducing IL-1 mRNA expression while no effect was observed on NLRP3 gene expression. RES and PD had no effect on crystal phagocytosis when used as pretreatment. Both polyphenols were significantly effective in inhibiting ROS and NO production. Our results demonstrated that RES and PD are effective in inhibiting crystal-induced inflammation. Data obtained after cell pretreatment allow us to hypothesize that these polyphenols act on specific signaling pathways, preventing inflammation.
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spelling pubmed-69154612019-12-24 Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells Oliviero, Francesca Zamudio-Cuevas, Yessica Belluzzi, Elisa Andretto, Lisa Scanu, Anna Favero, Marta Ramonda, Roberta Ravagnan, Giampietro López-Reyes, Alberto Spinella, Paolo Punzi, Leonardo Foods Article Resveratol (RES) and its natural precursor polydatin (PD) are polyphenols that may display a broad variety of beneficial effects including anti-inflammatory properties. This study aimed to investigate the role of RES and PD in the inflammatory process induced by monosodium urate (MSU) and calcium pyrophosphate (CPP) crystals in vitro. A monocytic cell line (THP-1) was primed for 3 hours with phorbol myristate acetate (100 ng/mL) and stimulated with synthetic MSU (0.05 mg/mL) and CPP (0.025 mg/mL) crystals. RES and PD were added to cultures concurrently with the crystals, or as 2-hour pretreatment. The effect of the two polyphenols was evaluated on intracellular and extracellular IL-1β levels, NACHT-LRRPYD-containing protein-3 (NLRP3) inflammasome expression, reactive oxygen species (ROS) and nitric oxide (NO) production, and the assessment of crystal phagocytosis. RES and PD strongly inhibited IL-1β induced by crystals after cell pretreatment. Cell pretreatment was effective also in reducing IL-1 mRNA expression while no effect was observed on NLRP3 gene expression. RES and PD had no effect on crystal phagocytosis when used as pretreatment. Both polyphenols were significantly effective in inhibiting ROS and NO production. Our results demonstrated that RES and PD are effective in inhibiting crystal-induced inflammation. Data obtained after cell pretreatment allow us to hypothesize that these polyphenols act on specific signaling pathways, preventing inflammation. MDPI 2019-11-07 /pmc/articles/PMC6915461/ /pubmed/31703439 http://dx.doi.org/10.3390/foods8110560 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oliviero, Francesca
Zamudio-Cuevas, Yessica
Belluzzi, Elisa
Andretto, Lisa
Scanu, Anna
Favero, Marta
Ramonda, Roberta
Ravagnan, Giampietro
López-Reyes, Alberto
Spinella, Paolo
Punzi, Leonardo
Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title_full Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title_fullStr Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title_full_unstemmed Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title_short Polydatin and Resveratrol Inhibit the Inflammatory Process Induced by Urate and Pyrophosphate Crystals in THP-1 Cells
title_sort polydatin and resveratrol inhibit the inflammatory process induced by urate and pyrophosphate crystals in thp-1 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915461/
https://www.ncbi.nlm.nih.gov/pubmed/31703439
http://dx.doi.org/10.3390/foods8110560
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