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Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array

Inertial separation techniques in a microfluidic system have been widely employed in the field of medical diagnosis for a long time. Despite no requirement of external forces, it requires strong hydrodynamic forces that could potentially cause cell damage or loss during the separation process. This...

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Autores principales: Suwannaphan, Thammawit, Srituravanich, Werayut, Sailasuta, Achariya, Piyaviriyakul, Prapruddee, Bhanpattanakul, Suchaya, Jeamsaksiri, Wutthinan, Sripumkhai, Witsaroot, Pimpin, Alongkorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915465/
https://www.ncbi.nlm.nih.gov/pubmed/31726665
http://dx.doi.org/10.3390/mi10110772
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author Suwannaphan, Thammawit
Srituravanich, Werayut
Sailasuta, Achariya
Piyaviriyakul, Prapruddee
Bhanpattanakul, Suchaya
Jeamsaksiri, Wutthinan
Sripumkhai, Witsaroot
Pimpin, Alongkorn
author_facet Suwannaphan, Thammawit
Srituravanich, Werayut
Sailasuta, Achariya
Piyaviriyakul, Prapruddee
Bhanpattanakul, Suchaya
Jeamsaksiri, Wutthinan
Sripumkhai, Witsaroot
Pimpin, Alongkorn
author_sort Suwannaphan, Thammawit
collection PubMed
description Inertial separation techniques in a microfluidic system have been widely employed in the field of medical diagnosis for a long time. Despite no requirement of external forces, it requires strong hydrodynamic forces that could potentially cause cell damage or loss during the separation process. This might lead to the wrong interpretation of laboratory results since the change of structures and functional characteristics of cells due to the hydrodynamic forces that occur are not taken into account. Therefore, it is important to investigate the cell viability and damage along with the separation efficacy of the device in the design process. In this study, two inertial separation techniques—spiral microchannel and contraction-expansion array (CEA)—were examined to evaluate cell viability, morphology and intracellular structures using a trypan blue assay (TB), Scanning Electron Microscopy (SEM) and Wright-Giemsa stain. We discovered that cell loss was not significantly found in a feeding system, i.e., syringe, needle and tube, but mostly occurred in the inertial separation devices while the change of cell morphology and intracellular structures were found in the feeding system and inertial separation devices. Furthermore, percentage of cell loss was not significant in both devices (7–10%). However, the change of cell morphology was considerably increased (30%) in spiral microchannel (shear stress dominated) rather than in CEA (12%). In contrast, the disruption of intracellular structures was increased (14%) in CEA (extensional and shear stress dominated equally) rather than spiral microchannel (2%). In these experiments, leukocytes of canine were used as samples because their sizes are varied in a range between 7–12 µm, and they are commonly used as a biomarker in many clinical and medical applications.
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spelling pubmed-69154652019-12-24 Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array Suwannaphan, Thammawit Srituravanich, Werayut Sailasuta, Achariya Piyaviriyakul, Prapruddee Bhanpattanakul, Suchaya Jeamsaksiri, Wutthinan Sripumkhai, Witsaroot Pimpin, Alongkorn Micromachines (Basel) Article Inertial separation techniques in a microfluidic system have been widely employed in the field of medical diagnosis for a long time. Despite no requirement of external forces, it requires strong hydrodynamic forces that could potentially cause cell damage or loss during the separation process. This might lead to the wrong interpretation of laboratory results since the change of structures and functional characteristics of cells due to the hydrodynamic forces that occur are not taken into account. Therefore, it is important to investigate the cell viability and damage along with the separation efficacy of the device in the design process. In this study, two inertial separation techniques—spiral microchannel and contraction-expansion array (CEA)—were examined to evaluate cell viability, morphology and intracellular structures using a trypan blue assay (TB), Scanning Electron Microscopy (SEM) and Wright-Giemsa stain. We discovered that cell loss was not significantly found in a feeding system, i.e., syringe, needle and tube, but mostly occurred in the inertial separation devices while the change of cell morphology and intracellular structures were found in the feeding system and inertial separation devices. Furthermore, percentage of cell loss was not significant in both devices (7–10%). However, the change of cell morphology was considerably increased (30%) in spiral microchannel (shear stress dominated) rather than in CEA (12%). In contrast, the disruption of intracellular structures was increased (14%) in CEA (extensional and shear stress dominated equally) rather than spiral microchannel (2%). In these experiments, leukocytes of canine were used as samples because their sizes are varied in a range between 7–12 µm, and they are commonly used as a biomarker in many clinical and medical applications. MDPI 2019-11-12 /pmc/articles/PMC6915465/ /pubmed/31726665 http://dx.doi.org/10.3390/mi10110772 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Suwannaphan, Thammawit
Srituravanich, Werayut
Sailasuta, Achariya
Piyaviriyakul, Prapruddee
Bhanpattanakul, Suchaya
Jeamsaksiri, Wutthinan
Sripumkhai, Witsaroot
Pimpin, Alongkorn
Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title_full Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title_fullStr Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title_full_unstemmed Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title_short Investigation of Leukocyte Viability and Damage in Spiral Microchannel and Contraction-Expansion Array
title_sort investigation of leukocyte viability and damage in spiral microchannel and contraction-expansion array
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915465/
https://www.ncbi.nlm.nih.gov/pubmed/31726665
http://dx.doi.org/10.3390/mi10110772
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