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Engineering a Bi-Conical Microchip as Vascular Stenosis Model

Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonst...

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Autores principales: Li, Yan, Wang, Jianchun, Wan, Wei, Chen, Chengmin, Wang, Xueying, Zhao, Pei, Hou, Yanjin, Tian, Hanmei, Wang, Jianmei, Nandakumar, Krishnaswamy, Wang, Liqiu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915513/
https://www.ncbi.nlm.nih.gov/pubmed/31752172
http://dx.doi.org/10.3390/mi10110790
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author Li, Yan
Wang, Jianchun
Wan, Wei
Chen, Chengmin
Wang, Xueying
Zhao, Pei
Hou, Yanjin
Tian, Hanmei
Wang, Jianmei
Nandakumar, Krishnaswamy
Wang, Liqiu
author_facet Li, Yan
Wang, Jianchun
Wan, Wei
Chen, Chengmin
Wang, Xueying
Zhao, Pei
Hou, Yanjin
Tian, Hanmei
Wang, Jianmei
Nandakumar, Krishnaswamy
Wang, Liqiu
author_sort Li, Yan
collection PubMed
description Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonstrated tunable dimensions, perfusable ability, and good cytocompatibility. The microchips showed blood-vessel-like lumens through fine embeddedness of human umbilical vein endothelial cells (HUVECs) on the interior surface of hydrogel microchannels, which closely reproduced the morphology and functions of human blood vessels. In the gradual narrowing region of bi-conical shape, fluid flow generated wall shear stress, which caused cell morphology variations. Wall shear rates at the gradual narrowing region were simulated by FLUENT software. The results showed that our microchannels qualified for performance as a vascular stenosis-like model in evaluating blood hydrodynamics. In general, our blood-vessel-on-a-chip could offer potential applications in the prevention, diagnosis, and therapy of arterial thrombosis.
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spelling pubmed-69155132019-12-24 Engineering a Bi-Conical Microchip as Vascular Stenosis Model Li, Yan Wang, Jianchun Wan, Wei Chen, Chengmin Wang, Xueying Zhao, Pei Hou, Yanjin Tian, Hanmei Wang, Jianmei Nandakumar, Krishnaswamy Wang, Liqiu Micromachines (Basel) Article Vascular stenosis is always associated with hemodynamic changes, especially shear stress alterations. Herein, bi-conical shaped microvessels were developed through flexibly and precisely controlled templated methods for hydrogel blood-vessel-like microchip. The blood-vessel-like microvessels demonstrated tunable dimensions, perfusable ability, and good cytocompatibility. The microchips showed blood-vessel-like lumens through fine embeddedness of human umbilical vein endothelial cells (HUVECs) on the interior surface of hydrogel microchannels, which closely reproduced the morphology and functions of human blood vessels. In the gradual narrowing region of bi-conical shape, fluid flow generated wall shear stress, which caused cell morphology variations. Wall shear rates at the gradual narrowing region were simulated by FLUENT software. The results showed that our microchannels qualified for performance as a vascular stenosis-like model in evaluating blood hydrodynamics. In general, our blood-vessel-on-a-chip could offer potential applications in the prevention, diagnosis, and therapy of arterial thrombosis. MDPI 2019-11-18 /pmc/articles/PMC6915513/ /pubmed/31752172 http://dx.doi.org/10.3390/mi10110790 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yan
Wang, Jianchun
Wan, Wei
Chen, Chengmin
Wang, Xueying
Zhao, Pei
Hou, Yanjin
Tian, Hanmei
Wang, Jianmei
Nandakumar, Krishnaswamy
Wang, Liqiu
Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title_full Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title_fullStr Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title_full_unstemmed Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title_short Engineering a Bi-Conical Microchip as Vascular Stenosis Model
title_sort engineering a bi-conical microchip as vascular stenosis model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915513/
https://www.ncbi.nlm.nih.gov/pubmed/31752172
http://dx.doi.org/10.3390/mi10110790
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