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Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System

Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus speci...

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Autores principales: Lin, Xue, Jin, Xiangyu, Xu, Bin, Wang, Ruliang, Fu, Rongxin, Su, Ya, Jiang, Kai, Yang, Han, Lu, Ying, Guo, Yong, Huang, Guoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915550/
https://www.ncbi.nlm.nih.gov/pubmed/31739456
http://dx.doi.org/10.3390/mi10110777
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author Lin, Xue
Jin, Xiangyu
Xu, Bin
Wang, Ruliang
Fu, Rongxin
Su, Ya
Jiang, Kai
Yang, Han
Lu, Ying
Guo, Yong
Huang, Guoliang
author_facet Lin, Xue
Jin, Xiangyu
Xu, Bin
Wang, Ruliang
Fu, Rongxin
Su, Ya
Jiang, Kai
Yang, Han
Lu, Ying
Guo, Yong
Huang, Guoliang
author_sort Lin, Xue
collection PubMed
description Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus species. The system is based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and consists of four specific LAMP primers, a disc microfluidic chip, and a portable real-time fluorescence detector. It could specifically and parallelly distinguish four species of the Ebola virus after only one sampling, including the Zaire Ebola virus, the Sudan Ebola virus, the Bundibugyo Ebola virus, and the Tai Forest Ebola virus, without cross-contamination. The limit of detection was as small as 10 copies per reaction, while the total consumption of sample and reagent was 0.94 μL per reaction. The final results could be obtained in 50 min after one addition of sample and reagent mixture. This approach provides simplicity, high sensitivity, and multi-target parallel detection at a low cost, which could enable convenient and effective on-site detections of the Ebola virus in the outdoors, remote areas, and modern hospitals.
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spelling pubmed-69155502019-12-24 Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System Lin, Xue Jin, Xiangyu Xu, Bin Wang, Ruliang Fu, Rongxin Su, Ya Jiang, Kai Yang, Han Lu, Ying Guo, Yong Huang, Guoliang Micromachines (Basel) Article Considering the lack of official vaccines and medicines for Ebola virus infection, reliable diagnostic methods are necessary for the control of the outbreak and the spread of the disease. We developed a microfluidic-chip-based portable system for fast and parallel detection of four Ebola virus species. The system is based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) and consists of four specific LAMP primers, a disc microfluidic chip, and a portable real-time fluorescence detector. It could specifically and parallelly distinguish four species of the Ebola virus after only one sampling, including the Zaire Ebola virus, the Sudan Ebola virus, the Bundibugyo Ebola virus, and the Tai Forest Ebola virus, without cross-contamination. The limit of detection was as small as 10 copies per reaction, while the total consumption of sample and reagent was 0.94 μL per reaction. The final results could be obtained in 50 min after one addition of sample and reagent mixture. This approach provides simplicity, high sensitivity, and multi-target parallel detection at a low cost, which could enable convenient and effective on-site detections of the Ebola virus in the outdoors, remote areas, and modern hospitals. MDPI 2019-11-14 /pmc/articles/PMC6915550/ /pubmed/31739456 http://dx.doi.org/10.3390/mi10110777 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Xue
Jin, Xiangyu
Xu, Bin
Wang, Ruliang
Fu, Rongxin
Su, Ya
Jiang, Kai
Yang, Han
Lu, Ying
Guo, Yong
Huang, Guoliang
Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title_full Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title_fullStr Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title_full_unstemmed Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title_short Fast and Parallel Detection of Four Ebola Virus Species on a Microfluidic-Chip-Based Portable Reverse Transcription Loop-Mediated Isothermal Amplification System
title_sort fast and parallel detection of four ebola virus species on a microfluidic-chip-based portable reverse transcription loop-mediated isothermal amplification system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915550/
https://www.ncbi.nlm.nih.gov/pubmed/31739456
http://dx.doi.org/10.3390/mi10110777
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