Cargando…
In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. Th...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915648/ https://www.ncbi.nlm.nih.gov/pubmed/31690040 http://dx.doi.org/10.3390/nano9111565 |
_version_ | 1783480065237975040 |
---|---|
author | Turin-Moleavin, Ioana-Andreea Fifere, Adrian Lungoci, Ana-Lacramioara Rosca, Irina Coroaba, Adina Peptanariu, Dragos Nastasa, Valentin Pasca, Sorin-Aurelian Bostanaru, Andra-Cristina Mares, Mihai Pinteala, Mariana |
author_facet | Turin-Moleavin, Ioana-Andreea Fifere, Adrian Lungoci, Ana-Lacramioara Rosca, Irina Coroaba, Adina Peptanariu, Dragos Nastasa, Valentin Pasca, Sorin-Aurelian Bostanaru, Andra-Cristina Mares, Mihai Pinteala, Mariana |
author_sort | Turin-Moleavin, Ioana-Andreea |
collection | PubMed |
description | Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications. |
format | Online Article Text |
id | pubmed-6915648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69156482019-12-24 In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates Turin-Moleavin, Ioana-Andreea Fifere, Adrian Lungoci, Ana-Lacramioara Rosca, Irina Coroaba, Adina Peptanariu, Dragos Nastasa, Valentin Pasca, Sorin-Aurelian Bostanaru, Andra-Cristina Mares, Mihai Pinteala, Mariana Nanomaterials (Basel) Article Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications. MDPI 2019-11-04 /pmc/articles/PMC6915648/ /pubmed/31690040 http://dx.doi.org/10.3390/nano9111565 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Turin-Moleavin, Ioana-Andreea Fifere, Adrian Lungoci, Ana-Lacramioara Rosca, Irina Coroaba, Adina Peptanariu, Dragos Nastasa, Valentin Pasca, Sorin-Aurelian Bostanaru, Andra-Cristina Mares, Mihai Pinteala, Mariana In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title | In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title_full | In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title_fullStr | In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title_full_unstemmed | In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title_short | In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates |
title_sort | in vitro and in vivo antioxidant activity of the new magnetic-cerium oxide nanoconjugates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915648/ https://www.ncbi.nlm.nih.gov/pubmed/31690040 http://dx.doi.org/10.3390/nano9111565 |
work_keys_str_mv | AT turinmoleavinioanaandreea invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT fifereadrian invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT lungocianalacramioara invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT roscairina invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT coroabaadina invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT peptanariudragos invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT nastasavalentin invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT pascasorinaurelian invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT bostanaruandracristina invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT maresmihai invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates AT pintealamariana invitroandinvivoantioxidantactivityofthenewmagneticceriumoxidenanoconjugates |