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In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates

Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. Th...

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Autores principales: Turin-Moleavin, Ioana-Andreea, Fifere, Adrian, Lungoci, Ana-Lacramioara, Rosca, Irina, Coroaba, Adina, Peptanariu, Dragos, Nastasa, Valentin, Pasca, Sorin-Aurelian, Bostanaru, Andra-Cristina, Mares, Mihai, Pinteala, Mariana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915648/
https://www.ncbi.nlm.nih.gov/pubmed/31690040
http://dx.doi.org/10.3390/nano9111565
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author Turin-Moleavin, Ioana-Andreea
Fifere, Adrian
Lungoci, Ana-Lacramioara
Rosca, Irina
Coroaba, Adina
Peptanariu, Dragos
Nastasa, Valentin
Pasca, Sorin-Aurelian
Bostanaru, Andra-Cristina
Mares, Mihai
Pinteala, Mariana
author_facet Turin-Moleavin, Ioana-Andreea
Fifere, Adrian
Lungoci, Ana-Lacramioara
Rosca, Irina
Coroaba, Adina
Peptanariu, Dragos
Nastasa, Valentin
Pasca, Sorin-Aurelian
Bostanaru, Andra-Cristina
Mares, Mihai
Pinteala, Mariana
author_sort Turin-Moleavin, Ioana-Andreea
collection PubMed
description Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications.
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spelling pubmed-69156482019-12-24 In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates Turin-Moleavin, Ioana-Andreea Fifere, Adrian Lungoci, Ana-Lacramioara Rosca, Irina Coroaba, Adina Peptanariu, Dragos Nastasa, Valentin Pasca, Sorin-Aurelian Bostanaru, Andra-Cristina Mares, Mihai Pinteala, Mariana Nanomaterials (Basel) Article Background. Cerium oxide nanoparticles present the mimetic activity of superoxide dismutase, being able to inactivate the excess of reactive oxygen species (ROS) correlated with a large number of pathologies, such as stents restenosis and the occurrence of genetic mutations that can cause cancer. This study presents the synthesis and biological characterisation of nanoconjugates based on nanoparticles of iron oxide interconnected with cerium oxide conjugates. Methods. The synthesis of magnetite-nanoceria nanoconjugates has been done in several stages, where the key to the process is the coating of nanoparticles with polyethyleneimine and its chemical activation-reticulation with glutaraldehyde. The nanoconjugates are characterised by several techniques, and the antioxidant activity was evaluated in vitro and in vivo. Results. Iron oxide nanoparticles interconnected with cerium oxide nanoparticles were obtained, having an average diameter of 8 nm. Nanoconjugates prove to possess superparamagnetic properties and the saturation magnetisation varies with the addition of diamagnetic components in the system, remaining within the limits of biomedical applications. In vitro free-radical scavenging properties of nanoceria are improved after the coating of nanoparticles with polyethylenimine and conjugation with magnetite nanoparticles. In vivo studies reveal increased antioxidant activity in all organs and fluids collected from mice, which demonstrates the ability of the nanoconjugates to reduce oxidative stress. Conclusion. Nanoconjugates possess magnetic properties, being able to scavenge free radicals, reducing the oxidative stress. The combination of the two properties mentioned above makes them excellent candidates for theranostic applications. MDPI 2019-11-04 /pmc/articles/PMC6915648/ /pubmed/31690040 http://dx.doi.org/10.3390/nano9111565 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Turin-Moleavin, Ioana-Andreea
Fifere, Adrian
Lungoci, Ana-Lacramioara
Rosca, Irina
Coroaba, Adina
Peptanariu, Dragos
Nastasa, Valentin
Pasca, Sorin-Aurelian
Bostanaru, Andra-Cristina
Mares, Mihai
Pinteala, Mariana
In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title_full In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title_fullStr In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title_full_unstemmed In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title_short In Vitro and In Vivo Antioxidant Activity of the New Magnetic-Cerium Oxide Nanoconjugates
title_sort in vitro and in vivo antioxidant activity of the new magnetic-cerium oxide nanoconjugates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915648/
https://www.ncbi.nlm.nih.gov/pubmed/31690040
http://dx.doi.org/10.3390/nano9111565
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