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Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP
Market transparency is in strong demand by consumers, and the authentication of species is an important step for seafood traceability. In this study, a simple molecular strategy, COIBar–RFLP (cytochrome oxidase I barcode–restriction fragment length polymorphism), is proposed to unveil commercial fra...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915651/ https://www.ncbi.nlm.nih.gov/pubmed/31683903 http://dx.doi.org/10.3390/foods8110537 |
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author | Ferrito, Venera Raffa, Alessandra Rossitto, Luana Federico, Concetta Saccone, Salvatore Pappalardo, Anna Maria |
author_facet | Ferrito, Venera Raffa, Alessandra Rossitto, Luana Federico, Concetta Saccone, Salvatore Pappalardo, Anna Maria |
author_sort | Ferrito, Venera |
collection | PubMed |
description | Market transparency is in strong demand by consumers, and the authentication of species is an important step for seafood traceability. In this study, a simple molecular strategy, COIBar–RFLP (cytochrome oxidase I barcode–restriction fragment length polymorphism), is proposed to unveil commercial fraud based on the practice of species substitution in the swordfish trade. In particular, COI barcoding allowed the identification of the species Prionace glauca, Mustelus mustelus, and Oxynotus centrina in slices labeled as Xiphias gladius. Furthermore, the enzymatic digestion of COI amplicons using the MboI restriction endonuclease allowed the simultaneous discrimination of the four species. Interestingly, an intraspecific differential MboI pattern was obtained for the swordfish samples. This pattern was useful to differentiate the two different clades revealed in this species by phylogenetic analyses using several molecular markers. These results indicate the need to strengthen regulations and define molecular tools for combating the occurrence of fraud along the seafood supply chain and show that COIBar–RFLP could become a standardized molecular tool to assess seafood authenticity. |
format | Online Article Text |
id | pubmed-6915651 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69156512019-12-24 Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP Ferrito, Venera Raffa, Alessandra Rossitto, Luana Federico, Concetta Saccone, Salvatore Pappalardo, Anna Maria Foods Article Market transparency is in strong demand by consumers, and the authentication of species is an important step for seafood traceability. In this study, a simple molecular strategy, COIBar–RFLP (cytochrome oxidase I barcode–restriction fragment length polymorphism), is proposed to unveil commercial fraud based on the practice of species substitution in the swordfish trade. In particular, COI barcoding allowed the identification of the species Prionace glauca, Mustelus mustelus, and Oxynotus centrina in slices labeled as Xiphias gladius. Furthermore, the enzymatic digestion of COI amplicons using the MboI restriction endonuclease allowed the simultaneous discrimination of the four species. Interestingly, an intraspecific differential MboI pattern was obtained for the swordfish samples. This pattern was useful to differentiate the two different clades revealed in this species by phylogenetic analyses using several molecular markers. These results indicate the need to strengthen regulations and define molecular tools for combating the occurrence of fraud along the seafood supply chain and show that COIBar–RFLP could become a standardized molecular tool to assess seafood authenticity. MDPI 2019-11-01 /pmc/articles/PMC6915651/ /pubmed/31683903 http://dx.doi.org/10.3390/foods8110537 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferrito, Venera Raffa, Alessandra Rossitto, Luana Federico, Concetta Saccone, Salvatore Pappalardo, Anna Maria Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title | Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title_full | Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title_fullStr | Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title_full_unstemmed | Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title_short | Swordfish or Shark Slice? A Rapid Response by COIBar–RFLP |
title_sort | swordfish or shark slice? a rapid response by coibar–rflp |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915651/ https://www.ncbi.nlm.nih.gov/pubmed/31683903 http://dx.doi.org/10.3390/foods8110537 |
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