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Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption

Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hyd...

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Autores principales: Sano, Emi, Mori, Chihiro, Matsuoka, Naoki, Ozaki, Yuka, Yagi, Keisuke, Wada, Aya, Tashima, Koichi, Yamasaki, Shinsuke, Tanabe, Kana, Yano, Kayo, Torisawa, Yu-suke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915658/
https://www.ncbi.nlm.nih.gov/pubmed/31752314
http://dx.doi.org/10.3390/mi10110793
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author Sano, Emi
Mori, Chihiro
Matsuoka, Naoki
Ozaki, Yuka
Yagi, Keisuke
Wada, Aya
Tashima, Koichi
Yamasaki, Shinsuke
Tanabe, Kana
Yano, Kayo
Torisawa, Yu-suke
author_facet Sano, Emi
Mori, Chihiro
Matsuoka, Naoki
Ozaki, Yuka
Yagi, Keisuke
Wada, Aya
Tashima, Koichi
Yamasaki, Shinsuke
Tanabe, Kana
Yano, Kayo
Torisawa, Yu-suke
author_sort Sano, Emi
collection PubMed
description Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. This drawback hinders widespread use of PDMS-based devices for drug discovery and development. Here, we describe a microfluidic cell culture system made of a tetrafluoroethylene-propylene (FEPM) elastomer. We demonstrated that FEPM does not absorb small hydrophobic compounds including rhodamine B and three types of drugs, nifedipine, coumarin, and Bay K8644, whereas PDMS absorbs them strongly. The device consists of two FEPM layers of microchannels separated by a thin collagen vitrigel membrane. Since FEPM is flexible and biocompatible, this microfluidic device can be used to culture cells while applying mechanical strain. When human umbilical vein endothelial cells (HUVECs) were subjected to cyclic strain (~10%) for 4 h in this device, HUVECs reoriented and aligned perpendicularly in response to the cyclic stretch. Moreover, we demonstrated that this device can be used to replicate the epithelial–endothelial interface as well as to provide physiological mechanical strain and fluid flow. This method offers a robust platform to produce organs-on-chips for drug discovery and development.
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spelling pubmed-69156582019-12-24 Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption Sano, Emi Mori, Chihiro Matsuoka, Naoki Ozaki, Yuka Yagi, Keisuke Wada, Aya Tashima, Koichi Yamasaki, Shinsuke Tanabe, Kana Yano, Kayo Torisawa, Yu-suke Micromachines (Basel) Article Organs-on-chips are microfluidic devices typically fabricated from polydimethylsiloxane (PDMS). Since PDMS has many attractive properties including high optical clarity and compliance, PDMS is very useful for cell culture applications; however, PDMS possesses a significant drawback in that small hydrophobic molecules are strongly absorbed. This drawback hinders widespread use of PDMS-based devices for drug discovery and development. Here, we describe a microfluidic cell culture system made of a tetrafluoroethylene-propylene (FEPM) elastomer. We demonstrated that FEPM does not absorb small hydrophobic compounds including rhodamine B and three types of drugs, nifedipine, coumarin, and Bay K8644, whereas PDMS absorbs them strongly. The device consists of two FEPM layers of microchannels separated by a thin collagen vitrigel membrane. Since FEPM is flexible and biocompatible, this microfluidic device can be used to culture cells while applying mechanical strain. When human umbilical vein endothelial cells (HUVECs) were subjected to cyclic strain (~10%) for 4 h in this device, HUVECs reoriented and aligned perpendicularly in response to the cyclic stretch. Moreover, we demonstrated that this device can be used to replicate the epithelial–endothelial interface as well as to provide physiological mechanical strain and fluid flow. This method offers a robust platform to produce organs-on-chips for drug discovery and development. MDPI 2019-11-19 /pmc/articles/PMC6915658/ /pubmed/31752314 http://dx.doi.org/10.3390/mi10110793 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sano, Emi
Mori, Chihiro
Matsuoka, Naoki
Ozaki, Yuka
Yagi, Keisuke
Wada, Aya
Tashima, Koichi
Yamasaki, Shinsuke
Tanabe, Kana
Yano, Kayo
Torisawa, Yu-suke
Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title_full Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title_fullStr Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title_full_unstemmed Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title_short Tetrafluoroethylene-Propylene Elastomer for Fabrication of Microfluidic Organs-on-Chips Resistant to Drug Absorption
title_sort tetrafluoroethylene-propylene elastomer for fabrication of microfluidic organs-on-chips resistant to drug absorption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915658/
https://www.ncbi.nlm.nih.gov/pubmed/31752314
http://dx.doi.org/10.3390/mi10110793
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