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An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging

Incorporating optical methods into implantable neural sensing devices is a challenging approach for brain–machine interfacing. Specifically, voltage-sensitive dye (VSD) imaging is a powerful tool enabling visualization of the network activity of thousands of neurons at high spatiotemporal resolution...

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Detalles Bibliográficos
Autores principales: Kunori, Nobuo, Takashima, Ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915684/
https://www.ncbi.nlm.nih.gov/pubmed/31752106
http://dx.doi.org/10.3390/mi10110789
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author Kunori, Nobuo
Takashima, Ichiro
author_facet Kunori, Nobuo
Takashima, Ichiro
author_sort Kunori, Nobuo
collection PubMed
description Incorporating optical methods into implantable neural sensing devices is a challenging approach for brain–machine interfacing. Specifically, voltage-sensitive dye (VSD) imaging is a powerful tool enabling visualization of the network activity of thousands of neurons at high spatiotemporal resolution. However, VSD imaging usually requires removal of the dura mater for dye staining, and thereafter the exposed cortex needs to be protected using an optically transparent artificial dura. This is a major disadvantage that limits repeated VSD imaging over the long term. To address this issue, we propose to use an atelocollagen membrane as the dura substitute. We fabricated a small cranial chamber device, which is a tubular structure equipped with a collagen membrane at one end of the tube. We implanted the device into rats and monitored neural activity in the frontal cortex 1 week following surgery. The results indicate that the collagen membrane was chemically transparent, allowing VSD staining across the membrane material. The membrane was also optically transparent enough to pass light; forelimb-evoked neural activity was successfully visualized through the artificial dura. Because of its ideal chemical and optical manipulation capability, this collagen membrane may be widely applicable in various implantable neural sensors.
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spelling pubmed-69156842019-12-24 An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging Kunori, Nobuo Takashima, Ichiro Micromachines (Basel) Communication Incorporating optical methods into implantable neural sensing devices is a challenging approach for brain–machine interfacing. Specifically, voltage-sensitive dye (VSD) imaging is a powerful tool enabling visualization of the network activity of thousands of neurons at high spatiotemporal resolution. However, VSD imaging usually requires removal of the dura mater for dye staining, and thereafter the exposed cortex needs to be protected using an optically transparent artificial dura. This is a major disadvantage that limits repeated VSD imaging over the long term. To address this issue, we propose to use an atelocollagen membrane as the dura substitute. We fabricated a small cranial chamber device, which is a tubular structure equipped with a collagen membrane at one end of the tube. We implanted the device into rats and monitored neural activity in the frontal cortex 1 week following surgery. The results indicate that the collagen membrane was chemically transparent, allowing VSD staining across the membrane material. The membrane was also optically transparent enough to pass light; forelimb-evoked neural activity was successfully visualized through the artificial dura. Because of its ideal chemical and optical manipulation capability, this collagen membrane may be widely applicable in various implantable neural sensors. MDPI 2019-11-18 /pmc/articles/PMC6915684/ /pubmed/31752106 http://dx.doi.org/10.3390/mi10110789 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kunori, Nobuo
Takashima, Ichiro
An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title_full An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title_fullStr An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title_full_unstemmed An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title_short An Implantable Cranial Window Using a Collagen Membrane for Chronic Voltage-Sensitive Dye Imaging
title_sort implantable cranial window using a collagen membrane for chronic voltage-sensitive dye imaging
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915684/
https://www.ncbi.nlm.nih.gov/pubmed/31752106
http://dx.doi.org/10.3390/mi10110789
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