Cargando…

AutoCLEM: An Automated Workflow for Correlative Live-Cell Fluorescence Microscopy and Cryo-Electron Tomography

Correlative light and electron microscopy (CLEM) combines the strengths of both light and electron imaging modalities and enables linking of biological spatiotemporal information from live-cell fluorescence light microscopy (fLM) to high-resolution cellular ultra-structures from cryo-electron micros...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fu, Xiaofeng, Ning, Jiying, Zhong, Zhou, Ambrose, Zandrea, Charles Watkins, Simon, Zhang, Peijun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915765/ https://www.ncbi.nlm.nih.gov/pubmed/31844138 http://dx.doi.org/10.1038/s41598-019-55766-8

Ejemplares similares

New hardware and workflows for semi-automated correlative cryo-fluorescence and cryo-electron microscopy/tomography
por: Schorb, Martin, et al.
Publicado: (2017)

Bridging length scales from molecules to the whole organism by cryoCLEM and cryoET
por: Lovatt, Megan, et al.
Publicado: (2022)

DeepCLEM: automated registration for correlative light and electron microscopy using deep learning
por: Seifert, Rick, et al.
Publicado: (2022)

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
por: Santarella-Mellwig, Rachel, et al.
Publicado: (2018)

Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans
por: Sexton, Danielle L., et al.
Publicado: (2021)

Correlative fluorescence microscopy, transmission electron microscopy and secondary ion mass spectrometry (CLEM-SIMS) for cellular imaging
por: Lange, Felix, et al.
Publicado: (2021)

Post-correlation on-lamella cryo-CLEM reveals the membrane architecture of lamellar bodies
por: Klein, Steffen, et al.
Publicado: (2021)

TEM, SEM, and STEM-based immuno-CLEM workflows offer complementary advantages
por: Oorschot, Viola, et al.
Publicado: (2021)

Improved Fluorescent Proteins for Dual-Colour Post-Embedding CLEM
por: Peng, Dingming, et al.
Publicado: (2022)

A modular platform for automated cryo-FIB workflows
por: Klumpe, Sven, et al.
Publicado: (2021)

HOPE-SIM, a cryo-structured illumination fluorescence microscopy system for accurately targeted cryo-electron tomography
por: Li, Shuoguo, et al.
Publicado: (2023)

One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM
por: Heiligenstein, Xavier, et al.
Publicado: (2022)

Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells
por: Tanida, Isei, et al.
Publicado: (2023)

SuperCLEM: an accessible correlative light and electron microscopy approach for investigation of neurons and glia in vitro
por: Booth, Daniel G., et al.
Publicado: (2019)

Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells
por: Hagen, Christoph, et al.
Publicado: (2012)

Fluorescence cryo-microscopy: current challenges and prospects
por: Kaufmann, Rainer, et al.
Publicado: (2014)

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
por: Sibert, Bryan S., et al.
Publicado: (2021)

AutoCryoPicker: an unsupervised learning approach for fully automated single particle picking in Cryo-EM images
por: Al-Azzawi, Adil, et al.
Publicado: (2019)

Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria
por: Peters, Simon, et al.
Publicado: (2021)

An Improved Procedure for Subcellular Spatial Alignment during Live-Cell CLEM
por: Padman, Benjamin S., et al.
Publicado: (2014)

Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy
por: Tao, Chang-Lu, et al.
Publicado: (2018)

Cryo-electron tomography to study viral infection
por: Graham, Miles, et al.
Publicado: (2023)

Advances in cryo-electron tomography and subtomogram averaging and classification
por: Zhang, Peijun
Publicado: (2019)

Integrated Fluorescence Microscopy (iFLM) for Cryo-FIB-milling and In-situ Cryo-ET
por: Yang, Jae, et al.
Publicado: (2023)

Indirect visualization of endogenous nuclear actin by correlative light and electron microscopy (CLEM) using an actin-directed chromobody
por: Abdellatif, Mohamed E. A., et al.
Publicado: (2019)

Correlative light and volume electron microscopy (vCLEM): How community participation can advance developing technologies
por: Guerin, Christopher J., et al.
Publicado: (2021)

A workflow for sizing oligomeric biomolecules based on cryo single molecule localization microscopy
por: Schneider, Magdalena C., et al.
Publicado: (2021)

Learning to automate cryo-electron microscopy data collection with Ptolemy
por: Kim, Paul T., et al.
Publicado: (2023)

Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins
por: Tanida, Isei, et al.
Publicado: (2020)

Super-Resolution Correlative Light and Electron Microscopy (SR-CLEM) Reveals Novel Ultrastructural Insights Into Dendritic Cell Podosomes
por: Joosten, Ben, et al.
Publicado: (2018)

In-resin CLEM of Epon-embedded cells using proximity labeling
por: Sanada, Takahito, et al.
Publicado: (2022)

Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells
por: Franken, Linda E., et al.
Publicado: (2022)

Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation
por: Sheibani, Sara, et al.
Publicado: (2021)

Correlative Light and Electron Microscopy (CLEM) Analysis of Nuclear Reorganization Induced by Clustered DNA Damage Upon Charged Particle Irradiation
por: Tonnemacher, Susanne, et al.
Publicado: (2020)

1.16 Cryo-Electron Microscopy and Tomography of Virus Particles
por: Rochat, R.H., et al.
Publicado: (2012)

Imaging endosomes and autophagosomes in whole mammalian cells using correlative cryo-fluorescence and cryo-soft X-ray microscopy (cryo-CLXM)()
por: Duke, Elizabeth M.H., et al.
Publicado: (2014)

Label-free 3D-CLEM Using Endogenous Tissue Landmarks
por: Luckner, Manja, et al.
Publicado: (2018)

Antibacterial and cytotoxic activities of naphthoquinone pigments from Onosma visianii Clem
por: Vukic, Milena D., et al.
Publicado: (2017)

Correlative cryo-fluorescence and cryo-scanning electron microscopy as a straightforward tool to study host-pathogen interactions
por: Strnad, Martin, et al.
Publicado: (2015)

The evolution of SPHIRE-crYOLO particle picking and its application in automated cryo-EM processing workflows
por: Wagner, Thorsten, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_o652uh8a6naqcl8j3c6582bpom