A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus

Gene-drive systems in diploid organisms bias the inheritance of one allele over another. CRISPR-based gene-drive expresses a guide RNA (gRNA) into the genome at the site where the gRNA directs Cas9-mediated cleavage. In the presence of Cas9, the gRNA cassette and any linked cargo sequences are copie...

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Detalles Bibliográficos
Autores principales: Valderrama, J. Andrés, Kulkarni, Surashree S., Nizet, Victor, Bier, Ethan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915771/
https://www.ncbi.nlm.nih.gov/pubmed/31844051
http://dx.doi.org/10.1038/s41467-019-13649-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915771/
https://www.ncbi.nlm.nih.gov/pubmed/31844051
http://dx.doi.org/10.1038/s41467-019-13649-6

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