Classification of mouse B cell types using surfaceome proteotype maps

System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and thr...

Descripción completa

Detalles Bibliográficos
Autores principales: van Oostrum, Marc, Müller, Maik, Klein, Fabian, Bruderer, Roland, Zhang, Hui, Pedrioli, Patrick G. A., Reiter, Lukas, Tsapogas, Panagiotis, Rolink, Antonius, Wollscheid, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915781/
https://www.ncbi.nlm.nih.gov/pubmed/31844046
http://dx.doi.org/10.1038/s41467-019-13418-5
_version_ 1783480096287358976
author van Oostrum, Marc
Müller, Maik
Klein, Fabian
Bruderer, Roland
Zhang, Hui
Pedrioli, Patrick G. A.
Reiter, Lukas
Tsapogas, Panagiotis
Rolink, Antonius
Wollscheid, Bernd
author_facet van Oostrum, Marc
Müller, Maik
Klein, Fabian
Bruderer, Roland
Zhang, Hui
Pedrioli, Patrick G. A.
Reiter, Lukas
Tsapogas, Panagiotis
Rolink, Antonius
Wollscheid, Bernd
author_sort van Oostrum, Marc
collection PubMed
description System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
format Online
Article
Text
id pubmed-6915781
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-69157812019-12-18 Classification of mouse B cell types using surfaceome proteotype maps van Oostrum, Marc Müller, Maik Klein, Fabian Bruderer, Roland Zhang, Hui Pedrioli, Patrick G. A. Reiter, Lukas Tsapogas, Panagiotis Rolink, Antonius Wollscheid, Bernd Nat Commun Article System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations. Nature Publishing Group UK 2019-12-16 /pmc/articles/PMC6915781/ /pubmed/31844046 http://dx.doi.org/10.1038/s41467-019-13418-5 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
van Oostrum, Marc
Müller, Maik
Klein, Fabian
Bruderer, Roland
Zhang, Hui
Pedrioli, Patrick G. A.
Reiter, Lukas
Tsapogas, Panagiotis
Rolink, Antonius
Wollscheid, Bernd
Classification of mouse B cell types using surfaceome proteotype maps
title Classification of mouse B cell types using surfaceome proteotype maps
title_full Classification of mouse B cell types using surfaceome proteotype maps
title_fullStr Classification of mouse B cell types using surfaceome proteotype maps
title_full_unstemmed Classification of mouse B cell types using surfaceome proteotype maps
title_short Classification of mouse B cell types using surfaceome proteotype maps
title_sort classification of mouse b cell types using surfaceome proteotype maps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915781/
https://www.ncbi.nlm.nih.gov/pubmed/31844046
http://dx.doi.org/10.1038/s41467-019-13418-5
work_keys_str_mv AT vanoostrummarc classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT mullermaik classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT kleinfabian classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT brudererroland classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT zhanghui classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT pedriolipatrickga classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT reiterlukas classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT tsapogaspanagiotis classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT rolinkantonius classificationofmousebcelltypesusingsurfaceomeproteotypemaps
AT wollscheidbernd classificationofmousebcelltypesusingsurfaceomeproteotypemaps

Ejemplares similares