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Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway

BACKGROUND: MicroRNA (miR)-containing exosomes released by acute myeloid leukemia (AML) cells can be delivered into hematopoietic progenitor cells to suppress normal hematopoiesis. Herein, our study was performed to evaluate the effect of exosomal miR-4532 secreted by AML cells on hematopoiesis of h...

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Autores principales: Zhao, Chen, Du, Feng, Zhao, Yang, Wang, Shanshan, Qi, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915875/
https://www.ncbi.nlm.nih.gov/pubmed/31842997
http://dx.doi.org/10.1186/s13287-019-1475-7
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author Zhao, Chen
Du, Feng
Zhao, Yang
Wang, Shanshan
Qi, Ling
author_facet Zhao, Chen
Du, Feng
Zhao, Yang
Wang, Shanshan
Qi, Ling
author_sort Zhao, Chen
collection PubMed
description BACKGROUND: MicroRNA (miR)-containing exosomes released by acute myeloid leukemia (AML) cells can be delivered into hematopoietic progenitor cells to suppress normal hematopoiesis. Herein, our study was performed to evaluate the effect of exosomal miR-4532 secreted by AML cells on hematopoiesis of hematopoietic stem cells. METHODS: Firstly, differentially expressed miRs related to AML were identified using microarray analysis. Subsequently, AML cell lines were collected, and CD34(+) HSCs were isolated from healthy pregnant women. Then, miR-4532 expression was measured in AML cells and AML cell-derived exosomes and CD34(+) HSCs, together with evaluation of the targeting relationship between miR-4532 and LDOC1. Then, AML cells were treated with miR-4532 inhibitor, and exosomes were separated from AML cells and co-cultured with CD34(+) HSCs. Gain- and loss-function approaches were employed in CD34(+) HSCs. Colony-forming units (CFU) and expression of dickkopf-1 (DKK1), a hematopoietic inhibiting factor associated with pathogenesis of AML, were determined in CD34(+) HSCs, as well as the extents of JAK2 and STAT3 phosphorylation and LDOC1 expression. RESULTS: miR-4532 was found to be upregulated in AML cells and AML cell-derived exosomes, while being downregulated in CD34(+) HSCs. In addition, exosomes released by AML cells targeted CD34(+) HSCs to decrease the expression of CFU and increase the expression of DKK1. miR-4532 was delivered into CD34(+) HSCs to target LDOC1 via AML cell-released exosomes. AML cell-derived exosomes containing miR-4532 inhibitor increased CFU but reduced DKK1 in CD34(+) HSCs. Inhibition of miR-4532 or JAK2, or ectopic expression of LDOC1 upregulated CFU and downregulated DKK1 expression as well as the extents of JAK2 and STAT3 phosphorylation in CD34(+) HSCs. CONCLUSION: In conclusion, AML cell-derived exosomes carrying miR-4532 repress normal HSC hematopoiesis via activation of the LDOC1-dependent STAT3 signaling pathway.
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spelling pubmed-69158752019-12-30 Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway Zhao, Chen Du, Feng Zhao, Yang Wang, Shanshan Qi, Ling Stem Cell Res Ther Research BACKGROUND: MicroRNA (miR)-containing exosomes released by acute myeloid leukemia (AML) cells can be delivered into hematopoietic progenitor cells to suppress normal hematopoiesis. Herein, our study was performed to evaluate the effect of exosomal miR-4532 secreted by AML cells on hematopoiesis of hematopoietic stem cells. METHODS: Firstly, differentially expressed miRs related to AML were identified using microarray analysis. Subsequently, AML cell lines were collected, and CD34(+) HSCs were isolated from healthy pregnant women. Then, miR-4532 expression was measured in AML cells and AML cell-derived exosomes and CD34(+) HSCs, together with evaluation of the targeting relationship between miR-4532 and LDOC1. Then, AML cells were treated with miR-4532 inhibitor, and exosomes were separated from AML cells and co-cultured with CD34(+) HSCs. Gain- and loss-function approaches were employed in CD34(+) HSCs. Colony-forming units (CFU) and expression of dickkopf-1 (DKK1), a hematopoietic inhibiting factor associated with pathogenesis of AML, were determined in CD34(+) HSCs, as well as the extents of JAK2 and STAT3 phosphorylation and LDOC1 expression. RESULTS: miR-4532 was found to be upregulated in AML cells and AML cell-derived exosomes, while being downregulated in CD34(+) HSCs. In addition, exosomes released by AML cells targeted CD34(+) HSCs to decrease the expression of CFU and increase the expression of DKK1. miR-4532 was delivered into CD34(+) HSCs to target LDOC1 via AML cell-released exosomes. AML cell-derived exosomes containing miR-4532 inhibitor increased CFU but reduced DKK1 in CD34(+) HSCs. Inhibition of miR-4532 or JAK2, or ectopic expression of LDOC1 upregulated CFU and downregulated DKK1 expression as well as the extents of JAK2 and STAT3 phosphorylation in CD34(+) HSCs. CONCLUSION: In conclusion, AML cell-derived exosomes carrying miR-4532 repress normal HSC hematopoiesis via activation of the LDOC1-dependent STAT3 signaling pathway. BioMed Central 2019-12-16 /pmc/articles/PMC6915875/ /pubmed/31842997 http://dx.doi.org/10.1186/s13287-019-1475-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhao, Chen
Du, Feng
Zhao, Yang
Wang, Shanshan
Qi, Ling
Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title_full Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title_fullStr Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title_full_unstemmed Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title_short Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway
title_sort acute myeloid leukemia cells secrete microrna-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the ldoc1-dependent stat3 signaling pathway
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915875/
https://www.ncbi.nlm.nih.gov/pubmed/31842997
http://dx.doi.org/10.1186/s13287-019-1475-7
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