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Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate

BACKGROUND: Peste des petits ruminants (PPR) is a viral disease of major economic importance on small ruminants. Goats are usually known to be more susceptible to the disease. Infection chronology, virus circulation, and the disease early detection need to be better understood. This study evaluates...

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Autores principales: Bamouh, Z., Fakri, F., Jazouli, M., Safini, N., Omari Tadlaoui, K., Elharrak, M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916009/
https://www.ncbi.nlm.nih.gov/pubmed/31842890
http://dx.doi.org/10.1186/s12917-019-2164-6
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author Bamouh, Z.
Fakri, F.
Jazouli, M.
Safini, N.
Omari Tadlaoui, K.
Elharrak, M.
author_facet Bamouh, Z.
Fakri, F.
Jazouli, M.
Safini, N.
Omari Tadlaoui, K.
Elharrak, M.
author_sort Bamouh, Z.
collection PubMed
description BACKGROUND: Peste des petits ruminants (PPR) is a viral disease of major economic importance on small ruminants. Goats are usually known to be more susceptible to the disease. Infection chronology, virus circulation, and the disease early detection need to be better understood. This study evaluates the tissue tropism and pathogenesis of PPR following experimental infection of goats using a lineage IV virus, the most dominant in the world originated from Asia. PPRV infection was experimentally induced in 4 six-month-old goats by intra-nasal and intravenous route of cell virus suspension and from infectious mashed tissue. The clinical signs were observed and goats were euthanized at predetermined clinical score level for post-mortem examinations and PPRV detection by RT-PCR. Clinical signs of infection were present, pyrexia, serous-mucopurulent nasal discharges, coughing, diarrhea and asthenia, for both cell virus suspension and infectious mashed tissue. PPRV genome was highly detected in swabs and tissues with clinical signs dominated by pulmonary attack and digestive symptoms secondary. RESULTS: Results of this study indicates that PPRV is an invasive infection in animals that in a short period, less than 10 days, invade all vital organs. On live animals, early diagnostic may be easily done on lacrimal and rectal swabs. CONCLUSION: The experimental PPRV-infection model using the cell virus suspension is suitable for vaccine evaluation as a standard model.
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spelling pubmed-69160092019-12-30 Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate Bamouh, Z. Fakri, F. Jazouli, M. Safini, N. Omari Tadlaoui, K. Elharrak, M. BMC Vet Res Research Article BACKGROUND: Peste des petits ruminants (PPR) is a viral disease of major economic importance on small ruminants. Goats are usually known to be more susceptible to the disease. Infection chronology, virus circulation, and the disease early detection need to be better understood. This study evaluates the tissue tropism and pathogenesis of PPR following experimental infection of goats using a lineage IV virus, the most dominant in the world originated from Asia. PPRV infection was experimentally induced in 4 six-month-old goats by intra-nasal and intravenous route of cell virus suspension and from infectious mashed tissue. The clinical signs were observed and goats were euthanized at predetermined clinical score level for post-mortem examinations and PPRV detection by RT-PCR. Clinical signs of infection were present, pyrexia, serous-mucopurulent nasal discharges, coughing, diarrhea and asthenia, for both cell virus suspension and infectious mashed tissue. PPRV genome was highly detected in swabs and tissues with clinical signs dominated by pulmonary attack and digestive symptoms secondary. RESULTS: Results of this study indicates that PPRV is an invasive infection in animals that in a short period, less than 10 days, invade all vital organs. On live animals, early diagnostic may be easily done on lacrimal and rectal swabs. CONCLUSION: The experimental PPRV-infection model using the cell virus suspension is suitable for vaccine evaluation as a standard model. BioMed Central 2019-12-16 /pmc/articles/PMC6916009/ /pubmed/31842890 http://dx.doi.org/10.1186/s12917-019-2164-6 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Bamouh, Z.
Fakri, F.
Jazouli, M.
Safini, N.
Omari Tadlaoui, K.
Elharrak, M.
Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title_full Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title_fullStr Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title_full_unstemmed Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title_short Peste des petits ruminants pathogenesis on experimental infected goats by the Moroccan 2015 isolate
title_sort peste des petits ruminants pathogenesis on experimental infected goats by the moroccan 2015 isolate
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916009/
https://www.ncbi.nlm.nih.gov/pubmed/31842890
http://dx.doi.org/10.1186/s12917-019-2164-6
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