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The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection

Verticillium dahliae is a soil‐borne fungus that causes vascular wilt on numerous plants worldwide. The fungus survives in the soil for up to 14 years by producing melanized microsclerotia. The protective function of melanin in abiotic stresses is well documented. Here, we found that the V. dahliae...

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Autores principales: Li, Jun‐Jiao, Zhou, Lei, Yin, Chun‐Mei, Zhang, Dan‐Dan, Klosterman, Steven J., Wang, Bao‐Li, Song, Jian, Wang, Dan, Hu, Xiao‐Ping, Subbarao, Krishna V., Chen, Jie‐Yin, Dai, Xiao‐Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916341/
https://www.ncbi.nlm.nih.gov/pubmed/31667948
http://dx.doi.org/10.1111/1462-2920.14846
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author Li, Jun‐Jiao
Zhou, Lei
Yin, Chun‐Mei
Zhang, Dan‐Dan
Klosterman, Steven J.
Wang, Bao‐Li
Song, Jian
Wang, Dan
Hu, Xiao‐Ping
Subbarao, Krishna V.
Chen, Jie‐Yin
Dai, Xiao‐Feng
author_facet Li, Jun‐Jiao
Zhou, Lei
Yin, Chun‐Mei
Zhang, Dan‐Dan
Klosterman, Steven J.
Wang, Bao‐Li
Song, Jian
Wang, Dan
Hu, Xiao‐Ping
Subbarao, Krishna V.
Chen, Jie‐Yin
Dai, Xiao‐Feng
author_sort Li, Jun‐Jiao
collection PubMed
description Verticillium dahliae is a soil‐borne fungus that causes vascular wilt on numerous plants worldwide. The fungus survives in the soil for up to 14 years by producing melanized microsclerotia. The protective function of melanin in abiotic stresses is well documented. Here, we found that the V. dahliae tetraspan transmembrane protein VdSho1, a homolog of the Saccharomyces cerevisiae Sho1, acts as an osmosensor, and is required for plant penetration and melanin biosynthesis. The deletion mutant ΔSho1 was incubated on a cellophane membrane substrate that mimics the plant epidermis, revealing that the penetration of ΔSho1 strain was reduced compared to the wild‐type strain. Furthermore, VdSho1 regulates melanin biosynthesis by a signalling mechanism requiring a kinase‐kinase signalling module of Vst50‐Vst11‐Vst7. Strains, ΔVst50, ΔVst7 and ΔVst11 also displayed defective penetration and melanin production like the ΔSho1 strain. Defects in penetration and melanin production in ΔSho1 were restored by overexpression of Vst50, suggesting that Vst50 lies downstream of VdSho1 in the regulatory pathway governing penetration and melanin biosynthesis. Data analyses revealed that the transmembrane portion of VdSho1 was essential for both membrane penetration and melanin production. This study demonstrates that Vst50‐Vst11‐Vst7 module regulates VdSho1‐mediated plant penetration and melanin production in V. dahliae, contributing to virulence.
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spelling pubmed-69163412019-12-17 The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection Li, Jun‐Jiao Zhou, Lei Yin, Chun‐Mei Zhang, Dan‐Dan Klosterman, Steven J. Wang, Bao‐Li Song, Jian Wang, Dan Hu, Xiao‐Ping Subbarao, Krishna V. Chen, Jie‐Yin Dai, Xiao‐Feng Environ Microbiol Research Articles Verticillium dahliae is a soil‐borne fungus that causes vascular wilt on numerous plants worldwide. The fungus survives in the soil for up to 14 years by producing melanized microsclerotia. The protective function of melanin in abiotic stresses is well documented. Here, we found that the V. dahliae tetraspan transmembrane protein VdSho1, a homolog of the Saccharomyces cerevisiae Sho1, acts as an osmosensor, and is required for plant penetration and melanin biosynthesis. The deletion mutant ΔSho1 was incubated on a cellophane membrane substrate that mimics the plant epidermis, revealing that the penetration of ΔSho1 strain was reduced compared to the wild‐type strain. Furthermore, VdSho1 regulates melanin biosynthesis by a signalling mechanism requiring a kinase‐kinase signalling module of Vst50‐Vst11‐Vst7. Strains, ΔVst50, ΔVst7 and ΔVst11 also displayed defective penetration and melanin production like the ΔSho1 strain. Defects in penetration and melanin production in ΔSho1 were restored by overexpression of Vst50, suggesting that Vst50 lies downstream of VdSho1 in the regulatory pathway governing penetration and melanin biosynthesis. Data analyses revealed that the transmembrane portion of VdSho1 was essential for both membrane penetration and melanin production. This study demonstrates that Vst50‐Vst11‐Vst7 module regulates VdSho1‐mediated plant penetration and melanin production in V. dahliae, contributing to virulence. John Wiley & Sons, Inc. 2019-11-24 2019-12 /pmc/articles/PMC6916341/ /pubmed/31667948 http://dx.doi.org/10.1111/1462-2920.14846 Text en © 2019 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Li, Jun‐Jiao
Zhou, Lei
Yin, Chun‐Mei
Zhang, Dan‐Dan
Klosterman, Steven J.
Wang, Bao‐Li
Song, Jian
Wang, Dan
Hu, Xiao‐Ping
Subbarao, Krishna V.
Chen, Jie‐Yin
Dai, Xiao‐Feng
The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title_full The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title_fullStr The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title_full_unstemmed The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title_short The Verticillium dahliae Sho1‐MAPK pathway regulates melanin biosynthesis and is required for cotton infection
title_sort verticillium dahliae sho1‐mapk pathway regulates melanin biosynthesis and is required for cotton infection
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6916341/
https://www.ncbi.nlm.nih.gov/pubmed/31667948
http://dx.doi.org/10.1111/1462-2920.14846
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