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Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development

Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in Drosophila called gonadal dysgenesis (GD). Harwich (Har) strains with P-elements cause severe GD in F1 daughters when Har fathers mate with mothers lacking P-element-piRNAs (i.e. ISO1 str...

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Autores principales: Srivastav, Satyam P, Rahman, Reazur, Ma, Qicheng, Pierre, Jasmine, Bandyopadhyay, Saptaparni, Lau, Nelson C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917496/
https://www.ncbi.nlm.nih.gov/pubmed/31845649
http://dx.doi.org/10.7554/eLife.49948
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author Srivastav, Satyam P
Rahman, Reazur
Ma, Qicheng
Pierre, Jasmine
Bandyopadhyay, Saptaparni
Lau, Nelson C
author_facet Srivastav, Satyam P
Rahman, Reazur
Ma, Qicheng
Pierre, Jasmine
Bandyopadhyay, Saptaparni
Lau, Nelson C
author_sort Srivastav, Satyam P
collection PubMed
description Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in Drosophila called gonadal dysgenesis (GD). Harwich (Har) strains with P-elements cause severe GD in F1 daughters when Har fathers mate with mothers lacking P-element-piRNAs (i.e. ISO1 strain). To address the mystery of why Har induces severe GD, we bred hybrid Drosophila with Har genomic fragments into the ISO1 background to create HISR-D or HISR-N lines that still cause Dysgenesis or are Non-dysgenic, respectively. In these lines, we discovered a highly truncated P-element variant we named ‘Har-P’ as the most frequent de novo insertion. Although HISR-D lines still contain full-length P-elements, HISR-N lines lost functional P-transposase but retained Har-P’s that when crossed back to P-transposase restores GD induction. Finally, we uncovered P-element-piRNA-directed repression on Har-P’s transmitted paternally to suppress somatic transposition. The Drosophila short Har-P’s and full-length P-elements relationship parallels the MITEs/DNA-transposase in plants and SINEs/LINEs in mammals.
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spelling pubmed-69174962019-12-18 Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development Srivastav, Satyam P Rahman, Reazur Ma, Qicheng Pierre, Jasmine Bandyopadhyay, Saptaparni Lau, Nelson C eLife Chromosomes and Gene Expression Without transposon-silencing Piwi-interacting RNAs (piRNAs), transposition causes an ovarian atrophy syndrome in Drosophila called gonadal dysgenesis (GD). Harwich (Har) strains with P-elements cause severe GD in F1 daughters when Har fathers mate with mothers lacking P-element-piRNAs (i.e. ISO1 strain). To address the mystery of why Har induces severe GD, we bred hybrid Drosophila with Har genomic fragments into the ISO1 background to create HISR-D or HISR-N lines that still cause Dysgenesis or are Non-dysgenic, respectively. In these lines, we discovered a highly truncated P-element variant we named ‘Har-P’ as the most frequent de novo insertion. Although HISR-D lines still contain full-length P-elements, HISR-N lines lost functional P-transposase but retained Har-P’s that when crossed back to P-transposase restores GD induction. Finally, we uncovered P-element-piRNA-directed repression on Har-P’s transmitted paternally to suppress somatic transposition. The Drosophila short Har-P’s and full-length P-elements relationship parallels the MITEs/DNA-transposase in plants and SINEs/LINEs in mammals. eLife Sciences Publications, Ltd 2019-12-17 /pmc/articles/PMC6917496/ /pubmed/31845649 http://dx.doi.org/10.7554/eLife.49948 Text en © 2019, Srivastav et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Chromosomes and Gene Expression
Srivastav, Satyam P
Rahman, Reazur
Ma, Qicheng
Pierre, Jasmine
Bandyopadhyay, Saptaparni
Lau, Nelson C
Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title_full Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title_fullStr Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title_full_unstemmed Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title_short Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila development
title_sort har-p, a short p-element variant, weaponizes p-transposase to severely impair drosophila development
topic Chromosomes and Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917496/
https://www.ncbi.nlm.nih.gov/pubmed/31845649
http://dx.doi.org/10.7554/eLife.49948
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